LOCUS       sequence-38173-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Walsh Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV-HA (New MCS)
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          128..224
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             288..314
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    359..493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(383..402)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     437..456
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(604..620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(617..639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    628..644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(652..658,659..676,677..682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    697..718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccggact ctagaggatc cggtactaga ggaactgaaa aaccagaaag
      121 ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggatccg gtggtggtgc
      181 aaatcaaaga actgctcctc agtggatgtt gcctttactt ctaggcctgt acggaagtgt
      241 tacttctgct ctaaaagctg cggaattgta cccgcgggcc caccatgtac ccatacgatg
      301 ttccagatta cgctcttatg gccatggagg aattcctcga ggatatcgcg gccgcgggga
      361 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      421 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      481 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      541 gtgggaggtt ttttcggatc ctctagagtc gatctgcagg catgctagct tggcgtaatc
      601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttc
//
