LOCUS       sequence-382012-        7391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MPP8-6xHis tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7391)
  TITLE     Leisegang-Brandes Plasmids
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV6-MPP8-6xHis
FEATURES             Location/Qualifiers
     source          1..7391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3614..3631
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3648..3664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3648..3664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3675..4297
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3874..3893)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(4446..5034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4526..4545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5363..5410
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5435..5454
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5642..6436)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5642..6436)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5753..5772)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6363..6382
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6471..6828)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6485..6620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6637..6828
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6785..6805
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(6830..6934)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6961..7389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7048..7067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7258..7279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccagt accgaggaga tctgcgccgc
     1021 gatcgccatg gagcaggttg cggagggagc aagggtgacc gcagtccctg tgtcagctgc
     1081 cgacagcact gaggagttgg ccgaagtcga agaaggagtt ggagtagtgg gcgaagataa
     1141 tgacgcagcc gcgagaggag cggaggcctt tggcgacagt gaggaggacg gagaggatgt
     1201 gttcgaggtg gagaagatcc tggacatgaa gaccgagggg ggtaaagttc tttacaaagt
     1261 tcgctggaaa ggctatacat cggatgatga tacctgggag cccgagattc acctggagga
     1321 ctgtaaagaa gtgcttcttg aatttaggaa gaaaattgca gagaacaaag ccaaagcagt
     1381 caggaaggat attcagagac tatccttaaa taacgacata tttgaggcga actctgatag
     1441 cgatcagcaa agtgagacaa aagaagatac ttccccaaag aagaaaaaga aaaaattgag
     1501 gcagagagaa gagaaaagcc cagatgatct gaaaaagaaa aaagcaaagg ccgggaagct
     1561 aaaagacaag tccaaaccag acctggagag ctccttggaa agtttagttt ttgatttaag
     1621 gacaaagaaa agaatttctg aagccaaaga agaactaaag gagtccaaaa agcccaaaaa
     1681 agatgaagta aaagaaacaa aagaattaaa gaaagttaaa aagggtgaaa taagagattt
     1741 aaagacgaaa acaagagaag atcccaaaga aaatagaaaa acaaaaaaag aaaaatttgt
     1801 cgaatcccag gtggaatctg aatcaagtgt acttaatgat tctccctttc cagaggatga
     1861 cagtgaaggg ctacattccg acagcagaga agagaaacaa aacactaaaa gtgcaagaga
     1921 gagagcaggg caggacatgg ggctggagca tggctttgag aagcccctag acagtgccat
     1981 gagtgctgag gaggataccg atgtcagagg caggaggaaa aagaagaccc cgagaaaggc
     2041 tgaggacact agagagaaca ggaagctaga gaacaagaac gctttcttag agaagaaaac
     2101 tgtgcctaaa aagcagagga atcaagacag aagcaaaagt gctgcagagt tagagaagct
     2161 gatgcctgta tctgcccaaa cgccaaaggg ccggaggttg agcggggaag agagaggcct
     2221 ctggtccacg gactcagccg aggaggacaa agaaaccaaa agaaatgaat ccaaagaaaa
     2281 atatcagaaa aggcatgatt ctgacaagga agaaaaaggc agaaaagagc caaaaggatt
     2341 aaagacactt aaggaaatca gaaatgcatt tgatttattt aaattaactc cagaagaaaa
     2401 aaatgatgtt tctgagaata atcggaaaag ggaagaaata ccactggatt ttaaaaccat
     2461 agacgatcac aaaaccaagg aaaacaaaca gtcacttaaa gaaaggagaa acaccagaga
     2521 cgaaacggat acttgggcat acattgctgc agaaggtgat caggaggttt tagacagcgt
     2581 gtgccaagca gatgagaatt cagatggcag gcagcagatt ctgagtttgg gcatggacct
     2641 gcagttggaa tggatgaagt tggaagattt ccaaaagcac cttgatggga aagatgagaa
     2701 ttttgctgca acagatgcaa ttccaagtaa tgtgttaagg gatgctgtga aaaatgggga
     2761 ttatattact gtaaaagttg cacttaattc aaatgaagaa tataacctgg accaagagga
     2821 ttccagtgga atgacactgg tgatgcttgc cgccgccgga gggcaggacg acctcctgcg
     2881 actcctcatc acaaaaggcg cgaaagtgaa cggtcggcag aagaacggga ccaccgccct
     2941 cattcatgct gcagagaaga actttttaac aacagtggct attcttttgg aagcaggagc
     3001 ttttgtaaat gtccagcaaa gcaatggtga gactgcactg atgaaggcct gtaaaagagg
     3061 aaattcagac atcgtacgac tcgtaattga atgtggagct gactgcaata ttttgtcaaa
     3121 gcaccagaat agtgccctgc actttgcgaa gcagtctaac aatgtgcttg tgtacgactt
     3181 gctgaagaac catttagaga cactttcaag agtagcagaa gagacaataa aggattactt
     3241 tgaagctcgc cttgctctgc tagaaccagt ttttccaatc gcatgtcatc gactctgtga
     3301 gggtccagat ttttcaacag atttcaatta caaaccccca cagaacatac cagaaggctc
     3361 tggcatcctg ctgtttatct tccatgcaaa ctttttgggt aaagaagtta ttgctcggct
     3421 ctgtggaccg tgtagtgtac aagctgtagt tctgaatgat aaatttcagc ttcctgtttt
     3481 tctggacagt cattttgttt actcattcag ccctgttgca ggtcccaata aactcttcat
     3541 aaggttgaca gaagcaccct ctgccaaggt taagttgcta ataggtgcat acagagtgca
     3601 gctgcagacg cgtcatcatc atcaccacca ctaaacggcc ggccgcggtc atagctgttt
     3661 cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     3721 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     3781 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     3841 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     3901 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     3961 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     4021 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     4081 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     4141 tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag acacagcata
     4201 ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc actgtcctct
     4261 catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca gcggcgagcg
     4321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4861 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5161 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg
     5221 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa
     5281 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc
     5341 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt
     5401 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     5461 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     5521 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     5581 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
     5641 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     5701 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     5761 gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca cagtcgatga
     5821 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     5881 cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg
     5941 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     6001 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     6061 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     6121 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     6181 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     6241 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     6301 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     6361 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     6421 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag
     6481 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     6541 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     6601 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     6661 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     6721 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     6781 ccacacccta actgacacac attccacagc tggttctttc cgcctcagga ctcttccttt
     6841 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6961 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7021 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7081 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7141 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7201 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7261 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7321 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7381 acgcgaattt t
//
