LOCUS       sequence-382103-        9942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-selection cassette plasmid for knocking-in at the 
            ChrII_ttTi5605 of the C. elegans genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Kurashina M, Mizumoto K
  TITLE     Targeting endogenous proteins for spatial and temporal knockdown 
            using auxin-inducible degron in Caenorhabditis elegans.
  JOURNAL   STAR Protoc. 2023 Jan 13;4(1):102028. doi: 
            10.1016/j.xpro.2022.102028.
  PUBMED    36640369
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2023-01-13"; volume: "4(1):102028. doi"; pages: " 
            10.1016/j.xpro.2022.102028"
COMMENT     Sequence Label: ChrII_ttTi5605_loxP_myo2_neoR
FEATURES             Location/Qualifiers
     source          1..9942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..47)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     360..382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     375..391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1344..1377)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2398..2422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2455..2483)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3231..3251
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4313..4329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5151..5945
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5205..5224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5815..5834
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(6675..6708)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7717..7733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7717..7733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7730..7752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7741..7757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7765..7771,7772..7789,7790..7795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7810..7831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7948..7965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8119..8707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8199..8218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8878..9669,9670..9738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9501..9520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9739..9843)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9911..9929
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      241 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      301 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      361 ccagtcacga cgttgtaaaa cgacggccag tgaattcccg cggtcgacgc aagcttctcc
      421 ttctcttcac atttctcaat aatttagctc taattgcatt cgtattcttc gcaaaaatac
      481 gtattctgat tgacccgatg aaatacggat gcctagttgc attgatcatc tacggttata
      541 cttatgggtg aggtttttca aatatatttt gatttaatcg aataatgaaa tttcagaact
      601 ggagttggtc caatttcgtg gtttatatcc tctgaactgg tacctcaaaa acatcgtagt
      661 attgctcaat ctgtggcata ttcaatgaat actgtaatgg tggttatcag tacatttaca
      721 gtattacctc tatattccgt tattggtagt tatgctttcc ttattctata ttcaattcct
      781 tcatttataa gtatgcttat attatttcgc tacttaccgg aaaccaaagg acgagagatt
      841 catgatattg tgaatgaact taaaaaaaaa tgaaaacctt tgaaaacgtt catacggccg
      901 aaattttact tgatcgagcc aattgaactt ttgttaagac ttacagaaaa gtgagagatt
      961 ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga aaaagaagaa aaatacaccc
     1021 gaagtatcat ataaaaatta aaaagattgt ttgacctggc ggaacttttt tgcaaaaact
     1081 ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc aaatgaatat caacaagaga
     1141 ttcttgaaga cgacgagcca cttgctcaga gcattgctta tctcgaatga gacccttgtt
     1201 aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt cctcctgatt ccatgatggt
     1261 agcaaactca cttcgtgtaa gtgcaagtaa gatcagtgtt tgtttcgata tcagtctgtt
     1321 tcggcccggg catcaggcgg cctataactt cgtatagcat acattatacg aagttattac
     1381 gtagagctcg gtacctcgta cgtaatacga ctcactagtc attttatatc tgagtagtac
     1441 ctttgcttta aatgtccata aaactaattt tataatcaat aaaacaacgt ttgtaaatca
     1501 actgagttta caagtagaga cattgaggga tactttcact atgctaaagt gataatcgac
     1561 caaataataa cttcactttg gtatttattc ctgtcttata aatgttatgt atgaattaaa
     1621 ttcatatgca tatggctcac tctgacaatt tttaataatc ttccagatca atattgacta
     1681 ccgatgcggg tggtcttttg ctttgaattc tgctgaactt tacaccccga acagcaatgt
     1741 gtgcttcagc ctaaaaaaaa gtaagtgtgt taatcagtgc ccccgattct tcattttttg
     1801 cccctctctc ccgtttcgtc ggcaaaagaa gagaaaataa agataagtct caagataggt
     1861 tggtaatcgc taaagtggtt gtgtggataa gagtagcaaa atggcaggaa gagcactttg
     1921 cgcgcacaca ctgtactcat tgttctggat aaaattctct cgttgtttgc cgtcggatgt
     1981 ctgcctctct gcattgagcc ggcttcttca ctatctttag ttaacctaaa atgccgtttc
     2041 ttttctcgta tccccactat cccgttgagg ttctctgctc tcttcgctcc ctaccgccag
     2101 cgagcaacta tccgtggggg cgccttgctc ggaagatggg ggggaagaaa gaagattttt
     2161 gctatttgca cttgagaaag agacttttcc tgcgtcgatg gtcagagaac agtgtgcaga
     2221 cacttttcag ctacctagaa ttacaattgg atatccccgc ctcccaatcc acccacccag
     2281 ggaaaaagaa gggctcgccg aaaatcaaag ttatctccag gctcgcgcat cccaccgagc
     2341 ggttgacttc tctccaccac ttttcatttt aaccctcgat cgtcagacac agaaatgaca
     2401 agtttgtaca aaaaagcagg cttaatgagt aaaggagaag aacttttcac tggagttgtc
     2461 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     2521 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     2581 ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     2641 tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca
     2701 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     2761 agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt
     2821 actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc
     2881 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     2941 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     3001 aatggaatca aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     3061 tttcagaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     3121 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     3181 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     3241 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa atagcattcg
     3301 tagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt caaaaataat
     3361 aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg agtaatattt
     3421 aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc
     3481 cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc
     3541 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     3601 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     3661 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta
     3721 cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     3781 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     3841 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     3901 gaaagtttat gaaattaatt ttcccgcttt tgctttttgg ggtttcccct attgtttgtc
     3961 aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     4021 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     4081 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     4141 taccaaacat aactgtttag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggaat
     4201 tcctgcagga tatctggatc cacgaagctt cccatggtga cgtcaccggt tctagatacc
     4261 taggtgagct ctggtaccct ctagtcaagg ccttaagtga gtcgtattac ggactggccg
     4321 tcgttttaca ggccggatgg gccatggcac cttaacattt ctaaatttta gtttaataaa
     4381 aaaattttaa aataatacga gttttgagac taactttcaa taaaaaccaa aaaattgctt
     4441 caaaaaagag ctcgcgccga tttacgataa acacagcgcg ctacgacact ccgaaaaccg
     4501 cgccgcacca ttacattctc cgccccaccg cacggtttgc ggaaggcgat gaaattgctg
     4561 ggcctaaaat tttaaccctt tttttcccgt ttttcaactg ttttcccgat tttttcgata
     4621 tttttttcag gtttttagtt agaaaagtat ataaaaattg cattatttgt agttttcgct
     4681 cgtaatttgt gatagaaaac ggccaatttg cctagatttg tgattctacc aagtggaatt
     4741 tttgggaaaa tatcaatatt ccacgatttt tattcaaatc ataacgtttc gaaattatca
     4801 gcttaaataa aactaaaata aataataaaa tactaaaaac cctgaatttc tcggttctcg
     4861 gttcagcttg agttcgttct atacacgtta tcgatttttg tcatttcgtt taatcatggg
     4921 ttttgattca ttttaaatat gaaatttgat ttattcctcg tttcgaagtt ggtttgtctt
     4981 gtccggccgg taaacaaatg ttattaaaaa aaataataaa tttcgaaatt tttcgtaatg
     5041 ttccgcaatc gtgtttttcc gtttatctcg tcttcccttc cttcaaatat actatttatt
     5101 ttctcaattc acatattttc agccagccct gctcaacaag tggaataaaa atgattgaac
     5161 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5221 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5281 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5341 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5401 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5461 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5521 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5581 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5641 ggctcgcacc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5701 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5761 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5821 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5881 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5941 tctgagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
     6001 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagc
     6061 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     6121 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     6181 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
     6241 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     6301 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     6361 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     6421 ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat
     6481 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     6541 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     6601 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttctcgag gtcgacaggc
     6661 ctgcatgcgt cgacataact tcgtatagca tacattatac gaagttatag ttgcaggacc
     6721 acttaacggt cttctgtata actacaaaaa agaataaaaa accgtatcac aactgagaaa
     6781 aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt
     6841 tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct cctgggcgtg
     6901 gaactcctta tcaagattac cattattgtc tgtcagaatt gatatttcaa ttatttcata
     6961 tctagacaaa cccgggtgtc ttgcatttat tagtactgtg agtttacata caaacgaggc
     7021 ttactcagat aacatagagt cacctctaat tagcaaaaac ccatttctaa ttagctattt
     7081 cgaccagttc taaagtcacc gtaattagaa atggtttttg tagcttttct aattagaaat
     7141 gaccccgaga taagtgaaat atggcatttt ctgggttttc tagtaagaaa tgatcccgca
     7201 cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa agtatattac acaattcaag
     7261 ttacattatt ttcaggaaac aatggcgaag agcaaagcag agattcaaaa ggaggaaaga
     7321 gacaacagat atgaacgaag acttcttgaa aagagaaaga gacatgaggt gagtaaccat
     7381 gataataaat aatcaagtaa gggctataga tttataaatt ctggagttaa cagtttcgaa
     7441 aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata ggggaatagc ctaaacgagt
     7501 gagaaaatta aatgtttgtt attttttcaa aaatgtctgc agggccgtta tcctttcaaa
     7561 tgcactctat caaacataga tcatatccat catatcttat ccagaattta tatcatatcc
     7621 aagattgaga gcgtttcaag gaaaatctca aacaaccata caattttgta gatcgacaac
     7681 aaggcctcca gcggccgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7921 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8101 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8521 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8641 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8701 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8761 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8821 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8881 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8941 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9001 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9061 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9121 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9181 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9241 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9301 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9361 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9421 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9481 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9541 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9601 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9661 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9721 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9781 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9841 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9901 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gc
//
