LOCUS       sequence-382173-       12517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human full-length LRRK2 with a lysosomal targeting 
            sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Kluss JH, Beilina A, Williamson CD, Lewis PA, Cookson MR, 
            Bonet-Ponce L
  TITLE     Lysosomal positioning regulates Rab10 phosphorylation at LRRK2(+) 
            lysosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2205492119. doi: 
            10.1073/pnas.2205492119. Epub 2022 Oct 18.
  PUBMED    36256825
REFERENCE   2  (bases 1 to 12517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2205492119. 
            doi"; pages: " 10.1073/pnas.2205492119. Epub 2022 Oct 18"
COMMENT     Sequence Label: LYSO-LRRK2
FEATURES             Location/Qualifiers
     source          1..12517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1057..1122
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    1159..1183
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8800..8824)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8858..9480
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9057..9076)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(9504..9524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9509..9838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9689..9824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9876..9895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9877..9895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9909..9925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9909..9925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9922..9944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9933..9949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9957..9963,9964..9981,9982..9987))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10002..10023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10140..10157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10311..10899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10391..10410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11070..11861,11862..11930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11693..11712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11931..12035)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12062..12490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12149..12168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12359..12380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gggtgctgct acagcagcga gaacgaggac
      961 tcggaccagg accgagagga gcggaagctg ctgctggacc ctagcagccc ccctaccaaa
     1021 gctctcaatg gagccgagcc caacggaggc agcggtgact acaaagacca tgacggtgat
     1081 tataaagatc atgacatcga ttacaaggat gacgatgaca agcttgcggc cgcgaattca
     1141 tcgatagatc tgatatcaac aagtttgtac aaaaaagcag gctccgaatt cgcccttatg
     1201 gctagtggca gctgtcaggg gtgcgaagag gacgaggaaa ctctgaagaa gttgatagtc
     1261 aggctgaaca atgtccagga aggaaaacag atagaaacgc tggtccaaat cctggaggat
     1321 ctgctggtgt tcacgtactc cgagcacgcc tccaagttat ttcaaggcaa aaatatccat
     1381 gtgcctctgt tgatcgtctt ggactcctat atgagagtcg cgagtgtgca gcaggtgggt
     1441 tggtcacttc tgtgcaaatt aatagaagtc tgtccaggta caatgcaaag cttaatggga
     1501 ccccaggatg ttggaaatga ttgggaagtc cttggtgttc accaattgat tcttaaaatg
     1561 ctaacagttc ataatgccag tgtaaacttg tcagtgattg gactgaagac cttagatctc
     1621 ctcctaactt caggtaaaat caccttgctg atattggatg aagaaagtga tattttcatg
     1681 ttaatttttg atgccatgca ctcatttcca gccaatgatg aagtccagaa acttggatgc
     1741 aaagctttac atgtgctgtt tgagagagtc tcagaggagc aactgactga atttgttgag
     1801 aacaaagatt atatgatatt gttaagtgcg ttaacaaatt ttaaagatga agaggaaatt
     1861 gtgcttcatg tgctgcattg tttacattcc ctagcgattc cttgcaataa tgtggaagtc
     1921 ctcatgagtg gcaatgtcag gtgttataat attgtggtgg aagctatgaa agcattccct
     1981 atgagtgaaa gaattcaaga agtgagttgc tgtttgctcc ataggcttac attaggtaat
     2041 tttttcaata tcctggtatt aaacgaagtc catgagtttg tggtgaaagc tgtgcagcag
     2101 tacccagaga atgcagcatt gcagatctca gcgctcagct gtttggccct cctcactgag
     2161 actattttct taaatcaaga tttagaggaa aagaatgaga atcaagagaa tgatgatgag
     2221 ggggaagaag ataaattgtt ttggctggaa gcctgttaca aagcattaac gtggcataga
     2281 aagaacaagc acgtgcagga ggccgcatgc tgggcactaa ataatctcct tatgtaccaa
     2341 aacagtttac atgagaagat tggagatgaa gatggccatt tcccagctca tagggaagtg
     2401 atgctctcca tgctgatgca ttcttcatca aaggaagttt tccaggcatc tgcgaatgca
     2461 ttgtcaactc tcttagaaca aaatgttaat ttcagaaaaa tactgttatc aaaaggaata
     2521 cacctgaatg ttttggagtt aatgcagaag catatacatt ctcccgaagt ggctgaaagt
     2581 ggctgtaaaa tgctaaatca tctttttgaa ggaagcaaca cttccctgga tataatggca
     2641 gcagtggtcc ccaaaatact aacagttatg aaacgtcatg agacatcatt accagtgcag
     2701 ctggaggcgc ttcgagctat tttacatttt atagtgcctg gcatgccaga agaatccagg
     2761 gaggatacag aatttcatca taagctaaat atggttaaaa aacagtgttt caagaatgat
     2821 attcacaaac tggtcctagc agctttgaac aggttcattg gaaatcctgg gattcagaaa
     2881 tgtggattaa aagtaatttc ttctattgta cattttcctg atgcattaga gatgttatcc
     2941 ctggaaggtg ctatggattc agtgcttcac acactgcaga tgtatccaga tgaccaagaa
     3001 attcagtgtc tgggtttaag tcttatagga tacttgatta caaagaagaa tgtgttcata
     3061 ggaactggac atctgctggc aaaaattctg gtttccagct tataccgatt taaggatgtt
     3121 gctgaaatac agactaaagg atttcagaca atcttagcaa tcctcaaatt gtcagcatct
     3181 ttttctaagc tgctggtgca tcattcattt gacttagtaa tattccatca aatgtcttcc
     3241 aatatcatgg aacaaaagga tcaacagttt ctaaacctct gttgcaagtg ttttgcaaaa
     3301 gtagctatgg atgattactt aaaaaatgtg atgctagaga gagcgtgtga tcagaataac
     3361 agcatcatgg ttgaatgctt gcttctattg ggagcagatg ccaatcaagc aaaggaggga
     3421 tcttctttaa tttgtcaggt atgtgagaaa gagagcagtc ccaaattggt ggaactctta
     3481 ctgaatagtg gatctcgtga acaagatgta cgaaaagcgt tgacgataag cattgggaaa
     3541 ggtgacagcc agatcatcag cttgctctta aggaggctgg ccctggatgt tgccaacaat
     3601 agcatttgcc ttggaggatt ttgtatagga aaagttgaac cttcttggct tggtccttta
     3661 tttccagata agacttctaa tttaaggaaa caaacaaata tagcatctac actagcaaga
     3721 atggtgatca gatatcagat gaaaagtgct gtggaagaag gaacagcctc aggcagcgat
     3781 ggaaattttt ctgaagatgt gctgtctaaa tttgatgaat ggacctttat tcctgactct
     3841 tctatggaca gtgtgtttgc tcaaagtgat gacctggata gtgaaggaag tgaaggctca
     3901 tttcttgtga aaaagaaatc taattcaatt agtgtaggag aattttaccg agatgccgta
     3961 ttacagcgtt gctcaccaaa tttgcaaaga cattccaatt ccttggggcc catttttgat
     4021 catgaagatt tactgaagcg aaaaagaaaa atattatctt cagatgattc actcaggtca
     4081 tcaaaacttc aatcccatat gaggcattca gacagcattt cttctctggc ttctgagaga
     4141 gaatatatta catcactaga cctttcagca aatgaactaa gagatattga tgccctaagc
     4201 cagaaatgct gtataagtgt tcatttggag catcttgaaa agctggagct tcaccagaat
     4261 gcactcacga gctttccaca acagctatgt gaaactctga agagtttgac acatttggac
     4321 ttgcacagta ataaatttac atcatttcct tcttatttgt tgaaaatgag ttgtattgct
     4381 aatcttgatg tctctcgaaa tgacattgga ccctcagtgg ttttagatcc tacagtgaaa
     4441 tgtccaactc tgaaacagtt taacctgtca tataaccagc tgtcttttgt acctgagaac
     4501 ctcactgatg tggtagagaa actggagcag ctcattttag aaggaaataa aatatcaggg
     4561 atatgctccc ccttgagact gaaggaactg aagattttaa accttagtaa gaaccacatt
     4621 tcatccctat cagagaactt tcttgaggct tgtcctaaag tggagagttt cagtgccaga
     4681 atgaattttc ttgctgctat gcctttcttg cctccttcta tgacaatcct aaaattatct
     4741 cagaacaaat tttcctgtat tccagaagca attttaaatc ttccacactt gcggtcttta
     4801 gatatgagca gcaatgatat tcagtaccta ccaggtcccg cacactggaa atctttgaac
     4861 ttaagggaac tcttatttag ccataatcag atcagcatct tggacttgag tgaaaaagca
     4921 tatttatggt ctagagtaga gaaactgcat ctttctcaca ataaactgaa agagattcct
     4981 cctgagattg gctgtcttga aaatctgaca tctctggatg tcagttacaa cttggaacta
     5041 agatcctttc ccaatgaaat ggggaaatta agcaaaatat gggatcttcc tttggatgaa
     5101 ctgcatctta actttgattt taaacatata ggatgtaaag ccaaagacat cataaggttt
     5161 cttcaacagc gattaaaaaa ggctgtgcct tataaccgaa tgaaacttat gattgtggga
     5221 aatactggga gtggtaaaac caccttattg cagcaattaa tgaaaaccaa gaaatcagat
     5281 cttggaatgc aaagtgccac agttggcata gatgtgaaag actggcctat ccaaataaga
     5341 gacaaaagaa agagagatct cgtcctaaat gtgtgggatt ttgcaggtcg tgaggaattc
     5401 tatagtactc atccccattt tatgacgcag cgagcattgt accttgctgt ctatgacctc
     5461 agcaagggac aggctgaagt tgatgccatg aagccttggc tcttcaatat aaaggctcgc
     5521 gcttcttctt cccctgtgat tctcgttggc acacatttgg atgtttctga tgagaagcaa
     5581 cgcaaagcct gcatgagtaa aatcaccaag gaactcctga ataagcgagg gttccctgcc
     5641 atacgagatt accactttgt gaatgccacc gaggaatctg atgctttggc aaaacttcgg
     5701 aaaaccatca taaacgagag ccttaatttc aagatccgag atcagcttgt tgttggacag
     5761 ctgattccag actgctatgt agaacttgaa aaaatcattt tatcggagcg taaaaatgtg
     5821 ccaattgaat ttcccgtaat tgaccggaaa cgattattac aactagtgag agaaaatcag
     5881 ctgcagttag atgaaaatga gcttcctcac gcagttcact ttctaaatga atcaggagtc
     5941 cttcttcatt ttcaagaccc agcactgcag ttaagtgact tgtactttgt ggaacccaag
     6001 tggctttgta aaatcatggc acagattttg acagtgaaag tggaaggttg tccaaaacac
     6061 cctaagggaa ttatttcgcg tagagatgtg gaaaaatttc tttcaaaaaa aaggaaattt
     6121 ccaaagaact acatgtcaca gtattttaag ctcctagaaa aattccagat tgctttgcca
     6181 ataggagaag aatatttgct ggttccaagc agtttgtctg accacaggcc tgtgatagag
     6241 cttccccatt gtgagaactc tgaaattatc atccgactat atgaaatgcc ttattttcca
     6301 atgggatttt ggtcaagatt aatcaatcga ttacttgaga tttcacctta catgctttca
     6361 gggagagaac gagcacttcg cccaaacaga atgtattggc gacaaggcat ttacttaaat
     6421 tggtctcctg aagcttattg tctggtagga tctgaagtct tagacaatca tccagagagt
     6481 ttcttaaaaa ttacagttcc ttcttgtaga aaaggctgta ttcttttggg ccaagttgtg
     6541 gaccacattg attctctcat ggaagaatgg tttcctgggt tgctggagat tgatatttgt
     6601 ggtgaaggag aaactctgtt gaagaaatgg gcattatata gttttaatga tggcgaagaa
     6661 catcaaaaaa tcttacttga tgacttgatg aagaaagcag aggaaggaga tctcttagta
     6721 aatccagatc aaccaaggct caccattcca atatctcaga ttgcccctga cttgattttg
     6781 gctgacctgc ctagaaatat tatgttgaat aatgatgagt tggaatttga acaagctcca
     6841 gagtttctcc taggtgatgg cagttttgga tcagtttacc gagcagccta tgaaggagaa
     6901 gaagtggctg tgaagatttt taataaacat acatcactca ggctgttaag acaagagctt
     6961 gtggtgcttt gccacctcca ccaccccagt ttgatatctt tgctggcagc tgggattcgt
     7021 ccccggatgt tggtgatgga gttagcctcc aagggttcct tggatcgcct gcttcagcag
     7081 gacaaagcca gcctcactag aaccctacag cacaggatcg cactccacgt agctgatggt
     7141 ttgagatacc tccactcagc catgattata taccgagacc tgaaacccca caatgtgctg
     7201 cttttcacac tgtatcccaa tgctgccatc atcgcaaaga ttgctgacta cggcattgct
     7261 cagtactgct gtagaatggg gataaaaaca tcagagggca caccagggtt tcgtgcacct
     7321 gaagttgcca gaggaaatgt catttataac caacaggctg atgtttattc atttggttta
     7381 ctactctatg acattttgac aactggaggt agaatagtag agggtttgaa gtttccaaat
     7441 gagtttgatg aattagaaat acaaggaaaa ttacctgatc cagttaaaga atatggttgt
     7501 gccccatggc ctatggttga gaaattaatt aaacagtgtt tgaaagaaaa tcctcaagaa
     7561 aggcctactt ctgcccaggt ctttgacatt ttgaattcag ctgaattagt ctgtctgacg
     7621 agacgcattt tattacctaa aaacgtaatt gttgaatgca tggttgctac acatcacaac
     7681 agcaggaatg caagcatttg gctgggctgt gggcacaccg acagaggaca gctctcattt
     7741 cttgacttaa atactgaagg atacacttct gaggaagttg ctgatagtag aatattgtgc
     7801 ttagccttgg tgcatcttcc tgttgaaaag gaaagctgga ttgtgtctgg gacacagtct
     7861 ggtactctcc tggtcatcaa taccgaagat gggaaaaaga gacataccct agaaaagatg
     7921 actgattctg tcacttgttt gtattgcaat tccttttcca agcaaagcaa acaaaaaaat
     7981 tttcttttgg ttggaaccgc tgatggcaag ttagcaattt ttgaagataa gactgttaag
     8041 cttaaaggag ctgctccttt gaagatacta aatataggaa atgtcagtac tccattgatg
     8101 tgtttgagtg aatccacaaa ttcaacggaa agaaatgtaa tgtggggagg atgtggcaca
     8161 aagattttct ccttttctaa tgatttcacc attcagaaac tcattgagac aagaacaagc
     8221 caactgtttt cttatgcagc tttcagtgat tccaacatca taacagtggt ggtagacact
     8281 gctctctata ttgctaagca aaatagccct gttgtggaag tgtgggataa gaaaactgaa
     8341 aaactctgtg gactaataga ctgcgtgcac tttttaaggg aggtaatggt aaaagaaaac
     8401 aaggaatcaa aacacaaaat gtcttattct gggagagtga aaaccctctg ccttcagaag
     8461 aacactgctc tttggatagg aactggagga ggccatattt tactcctgga tctttcaact
     8521 cgtcgactta tacgtgtaat ttacaacttt tgtaattcgg tcagagtcat gatgacagca
     8581 cagctaggaa gccttaaaaa tgtcatgctg gtattgggct acaaccggaa aaatactgaa
     8641 ggtacacaaa agcagaaaga gatacaatct tgcttgaccg tttgggacat caatcttcca
     8701 catgaagtgc aaaatttaga aaaacacatt gaagtgagaa aagaattagc tgaaaaaatg
     8761 agacgaacat ctgttgagta agagagaagg gcgaattcga cccagctttc ttgtacaaag
     8821 tggttgatat cggtaccagt cgactctaga ggatcccggg tggcatccct gtgacccctc
     8881 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     8941 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     9001 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     9061 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     9121 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     9181 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     9241 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     9301 acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca
     9361 ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag
     9421 ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt
     9481 gggtacgcgg ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     9541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9841 gctcctcgag gaactgaaaa accagaaagt taattcccta tagtgagtcg tattaaattc
     9901 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9961 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10021 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    10081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    10141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    10201 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10261 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10321 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10381 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10441 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10621 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10681 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10741 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10921 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10981 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11041 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11101 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11161 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11221 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11281 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11341 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11401 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11461 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11521 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11581 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11641 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11701 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11761 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11821 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11881 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11941 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12001 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    12061 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    12121 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    12181 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    12241 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    12301 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    12361 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    12421 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    12481 aacgcgaatt ttaacaaaat attaacgctt acaattt
//
