LOCUS       sequence-382414-       19950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deletion of DHPH domain of VPS13D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19950)
  AUTHORS   Guillen-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli
            P
  TITLE     VPS13D bridges the ER to mitochondria and peroxisomes via Miro.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212021. doi: 
            10.1083/jcb.202010004.
  PUBMED    33891013
REFERENCE   2  (bases 1 to 19950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212021. doi"; 
            pages: " 10.1083/jcb.202010004"
COMMENT     Sequence Label: VPS13D(∆ATG2-C/PH)^EGFP
FEATURES             Location/Qualifiers
     source          1..19950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        538..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     692..712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     738..762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5586..5595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5592..5594,5595..5597,5598..6308)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5592..5594,5595..5597,5598..6308)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5637..5658)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5898..5917)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6245..6266
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    14834..15422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14887..14907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15305..15316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(15444..15463)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15488..15536
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      15738..16166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15825..15844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16035..16056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(16175..16195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        16180..16509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      16360..16495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     16422..16441
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             16576..17370
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(16630..16649)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     17240..17259
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    17546..17667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(17583..17602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     17637..17656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(17716..17732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17716..17732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(17729..17751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    17740..17756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(17764..17770,17771..17788,17789..17794))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17809..17830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(17947..17964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18118..18706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18198..18217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18877..19668,19669..19737))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19500..19519
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19738..19842)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(19917..19936)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
       61 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      121 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      541 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      601 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      661 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      721 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
      781 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagaatgc
      841 tggagggcct ggtcgcttgg gtcctgaaca cctacctggg gaagtatgtg aacaacctga
      901 acaccgatca gctgtccgtc gctctgctga agggcgccgt ggagctggag aacctgccac
      961 tgaagaagga tgccctgaag gagctggagc tgcccttcga ggtgaaggcc ggctttatcg
     1021 gcaaggtgac cctgcagatc cctttttaca ggccacacgt ggacccctgg gtcatcagca
     1081 tcagctccct gcacctgatc ggcgcccctg agaagatcca ggacttcaat gatgagaagg
     1141 agaagctgct ggagagagag aggaagaagg ccctgctgca ggccctggag gagaagtgga
     1201 agaacgaccg ccagcagaag ggcgagtcct actggtattc tgtgaccgcc agcgtggtga
     1261 caagaatcgt ggagaatatc gagctgaaga tccaggacgt gcacctgagg ttcgaggatg
     1321 gcgtgaccaa cccttctcac ccattcgcct ttggcatctg catcaagaat gtgagcatgc
     1381 agaatgccgt gaacgagccc gtgcagaagc tgatgcggaa gaagcagctg gatgtggccg
     1441 agttttccat ctactgggac gtggattgta cactgctggg cgacctgcct cagatggagc
     1501 tgcaggaggc aatggcacgc agcatggaga gccggtccca ccactatgtg ctggagccag
     1561 tgttcgccag cgccctgctg aagcgcaact gctccaagaa gcctctgcgc tctcggcaca
     1621 gcccacggat cgactgtgat atccagctgg agacaatccc cctgaagctg tcccagctgc
     1681 agtacagaca gatcatggag tttctgaagg agctggagcg caaggagcgg caggtgaagt
     1741 tccggagatg gaagcctaag gtggccatca gcaagaactg ccgcgagtgg tggtatttcg
     1801 ccctgaacgc caatctgtat gagatccggg agcagagaaa gaggtgtacc tgggacttta
     1861 tgctgcacag ggcaagggac gccgtgtctt acaccgataa gtatttcaat aagctgaagg
     1921 gcggcctgct gagcacagac gataaggagg agatgtgccg gatcgaggag gagcagtcct
     1981 ttgaggagct gaagatcctg cgcgagctgg tgcacgatcg gttccacaag caggaggagc
     2041 tggccgagtc tctgagagag ccccagtttg acagccctgg cgcctgtcca ggagcaccag
     2101 agcctggagg aggctccgga atgctgcagt acctgcagtc ttggttccct ggatggggag
     2161 gatggtatgg acagcagacc ccagagggaa acgtggtgga gggcctgtcc gccgagcagc
     2221 aggagcagtg gattcccgag gagatcctgg gcaccgagga gttctttgac ccaacagccg
     2281 atgccagctg catgaacaca tacaccaagc gcgaccacgt gttcgccaag ctgaatctgc
     2341 agctgcagcg gggcacagtg accctgctgc acaaggagca gggaacccca cagatgaacg
     2401 agagcgcctt catgcagctg gagttttccg atgtgaagct gctggccgag tctctgccta
     2461 ggcgcaattc tagcctgctg agcgtgcgcc tgggaggcct gtttctgcgg gacctggcaa
     2521 cagagggcac catgttcccc ctgctggtgt ttccaaaccc ccagaaggaa gtgggcagag
     2581 tgtctcagag cttcggcctg cagaccacat ccgccgacag gtctgatcac tacccagcag
     2641 ccgaccccga tggccccgtg ttcgagatgc tgtatgagag aaatcccgcc cacagccact
     2701 tcgagcggag actgaacgtg tccaccaggc ccctgaatat catctacaac cctcaggcca
     2761 tcaagaaggt ggccgacttc ttttacaagg gcaaggtgca cacatctggc ttcggctatc
     2821 agagcgagct ggagctgaga gtggcagagg cagcaaggcg ccagtataac aagctgaaga
     2881 tgcagaccaa ggccgagatc agacagacac tggacaggct gctggtgggc gacttcatcg
     2941 aggagtctaa gcggtggacc gtgcggctgg atatcagcgc cccacaggtc atcttccccg
     3001 acgatttcaa gtttaagaat cctgtgctgg tggtggtgga cctgggcaga atgctgctga
     3061 caaataccca ggataacagc cggagaaagt ccagggacgg ctccgcctct gaggagacac
     3121 agttctccga cgatgagtac aagacaccac tggccacccc ccctaataca ccaccccctg
     3181 agtcctctag ctccaacggc gagaagaccc cacccttctc cggcgtggag ttttctgagg
     3241 agcagctgca ggcccacctg atgagcacaa agatgtacga gcggtacagc ctgtccttta
     3301 tggatctgca gatcatggtg ggccgggtga aggacaactg gaagcacgtg caggacatcg
     3361 atgtgggccc cacccacgtg gtggagaagt tcaatgtgca cctgcagctg gagaggcgcc
     3421 tgatctacac atccgaccca aagtatccag gagccgtgct gtctggaaac ctgcctgatc
     3481 tgaagatcca catcaatgag gacaagatca gcgccctgaa gaactgcttt gccctgctga
     3541 ccacaccaga gatgaagaca tccgataccc agatcaagga gaagatcttc ccacaggagg
     3601 agcagagggg ctctctgcag gacagcgtga tgaacctgac ccagtctatc gtgctgctgg
     3661 agcagcacac aagagaggtg ctggtggaga gccagctgct gctggccgag ttcaaggtca
     3721 attgtatgca gctgggcgtg gagtccaacg gcagatacat ctctgtgctg aaggtgttcg
     3781 gcaccaacgc ccactttgtg aagaggccat atgatgccga ggtgagcctg acagtgcacg
     3841 gcctgctgct ggtggacaca atgcagacct acggcgccga ctttgatctg ctgatggcca
     3901 gccacaagaa tctgtccttc gatatcccca ccggctccct gagagactct agggcacagt
     3961 cccccgtgtc tggacctaat gtggcacacc tgaccgacgg agccacactg aacgataggt
     4021 ctgccacctc tgtgagcctg gacaagatcc tgacaaagga gcaggagagc ctgatcaagc
     4081 tggagtatca gttcgtgtct agcgagtgcc cctctatgaa cctggatagc accctgcagg
     4141 tcatcagcct gcaggtgaac aatctggaca tcatcctgaa tcctgagaca atcgtggagc
     4201 tgatcggctt cctgcagaag tcttttccaa aggagaagga cgatctgagc cctcagccac
     4261 tgatgaccga tttcgagcgg agcttccggg agcagggcac ctaccagtct acatatgagc
     4321 agaataccga ggtggccgtg gagatccaca gactgaacct gctgctgctg aggacagtgg
     4381 gcatggccaa tcgcgagaag tacggaagga agatcgcaac cgcaagcatc ggcggcacaa
     4441 aagtgaatgt gagcatgggc tccacctttg acatgaacgg ctccctgggc tgcctgcagc
     4501 tgatggacct gacacaggat aatgtgaaga accagtatgt ggtgagcatc ggcaattccg
     4561 tgggctacga gaacatcatc tctgatatcg gctatttcga gagcgtgttt gtgcgcatgg
     4621 aggacgccgc cctgaccgag gccctgtcct tcacatttgt ggagcggtct aagcaggagt
     4681 gtttcctgaa tctgaagatg gccagcctgc actacaacca ctccgccaag tttctgaagg
     4741 agctgaccct gtctatggac gagctggagg agaacttcag gggcatgctg aagagcgccg
     4801 ccacaaaggt gaccacagtg ctggccacaa agaccgccga gtactctgag atggtgagcc
     4861 tgttcgagac acctagaaag acaagggagc ccttcatcct ggaggagaat gagatctatg
     4921 gcttcgacct ggcctcctct cacctggata ccgtgaagct gatcctgaac atcaatatcg
     4981 agtcccccgt ggtgtctatc cctaggaagc caggctcccc cgagctgctg gtgggacacc
     5041 tgggacagat cttcatccag aactttgtgg ccggcgacga tgagtcccgc tctgatcggc
     5101 tgcaggtgga gatcaaggac atcaagctgt acagcctgaa ttgcacccag ctggcaggaa
     5161 gagaggcagt gggctctgag ggcagcagga tgttttgtcc tccaagcggc tccggctctg
     5221 ccaactccca ggaggaggca cacttcacac gccacgactt ctttgagtct ctgcaccggg
     5281 gccaggcctt tcacatcctg aacaatacca caatccagtt caagctggag aagatcccaa
     5341 tcgagcggga gagcgagctg acctttagcc tgtcccccga cgatctgggc acaagctcca
     5401 tcatgaagat cgagggcaag ttcgtgaatc cagtgcaggt ggtgctggcc aagcacgtgt
     5461 acgagcaggt gctgcagacc ctggacaacc tggtgtacag cgaggatctg aataagtatc
     5521 cagcatccgc cacatctagc ccatgccctg attccccact gccaggctct ggctcctctg
     5581 gatccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5641 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5701 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5761 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     5821 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5881 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5941 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6001 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     6061 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     6121 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6181 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6241 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6301 tgtacaagtc cggactcaga tctggatccg gaggacccct gtctacatgc ggcgagtcta
     6361 gcgtggagag aaaggagaat ggcctgtttt ctcactcctc tctgtccaac acctctcaga
     6421 agtctctgag cgtgaaggag gtgaagagct tcacccagat ccaggccaca ttttgtatca
     6481 gcgagctgca ggtgcagctg tccggcgatc tgaccctggg agcacagggc ctggtgtctc
     6541 tgaagttcca ggattttgag gtggagttca gcaaggacca cccccagaca ctgtctatcc
     6601 agatcgccct gcacagcctg ctgatggagg acctgctgga gaagaatcct gatagcaagt
     6661 acaagaacct gatggtgtcc aggggagcac caaagcctag ctccctggca cagaaggagt
     6721 acctgtccca gtcttgccct agcgtgtcca acgtggaata tcctgacatg ccacgcagcc
     6781 tgccatccca catggaggag gcccccaacg tgttccagct gtaccagcgc cctacctctg
     6841 ccagccggaa gaagcagaag gaggtgcagg acaaggatta tccactgacc ccacccccta
     6901 gccctacagt ggatgagcca aagatcctgg tgggcaagag caagtttgac gattccctgg
     6961 tgcacatcaa catcttcctg gtggacaaga agcacccaga gttttctagc tcctacaata
     7021 gagtgaacag gtccatcgac gtggatttca attgtctgga cgtgctgatc acactgcaga
     7081 cctgggtggt catcctggac ttcttcggaa tcggcagcac cgcagacaac cacgccatga
     7141 gactgccacc cgagggcatc ctgcacaatg tgaagctgga gccccacgcc tctatggaga
     7201 gcggcctgca ggaccctgtg aacacaaagc tggatctgaa ggtgcactcc ctgtctctgg
     7261 tgctgaataa gaccacatct gagctggcca aggccaacgt gagcaagctg gtggcccacc
     7321 tggagatgat cgagggcgat ctggccctgc agggctccat cggcagcctg tccctgtctg
     7381 acctgacctg ccacggcgag ttctacagag agaggtttac cacaagcggc gaggaggccc
     7441 tgatcttcca gaccttcaag tatggccggc cagatcccct gctgaggaga gagcacgaca
     7501 tccgcgtgtc tctgcggatg gccagcgtgc agtacgtgca cacccagaga ttccaggccg
     7561 aggtggtggc cttcatccag cactttacac agctgcagga tgtgctgggc cgccagaggg
     7621 cagcaatcga gggacagacc gtgagggatc aggcacagcg gtgctcccgg gtgctgctgg
     7681 acatcgaggc aggagccccc gtgctgctga tccctgagtc tagccgcagc aacaatctga
     7741 tcgtggccaa cctgggcaag ctgaaggtga agaataagtt cctgtttgcc ggcttccccg
     7801 gcaccttttc tctgcaggac aaggagagcg tgccaagcgc ctcccctaca ggcatcccaa
     7861 agcactccct gagaaagacc acatctaccg aggagccaag gggaacacac tcccagggcc
     7921 agtttaccat gcctctggcc ggcatgtccc tgggctctct gaagagcgag ttcgtgccat
     7981 ctacaagcac caagcagcag ggccctcagc caacactgag cgtgggacag gagtcctcta
     8041 gcccagagga tcacgtgtgc ctgctggact gcgtggtggt ggatctgcag gacatggata
     8101 tcttcgccgc cgagagacac cctagggagt acagcaaggc cccagaggac tcctctggcg
     8161 atctgatctt cccctcttat tttgtgaggc agaccggcgg cagcctgctg acagagcctt
     8221 gccgcctgaa gctgcaggtg gagcggaacc tggataagga gatctcccac accgtgcctg
     8281 acatctctat ccacggcaat ctgagctccg tgcactgttc cctggacctg tacaagtata
     8341 agctgatccg cggcctgctg gagaacaatc tgggcgagcc aatcgaggag ttcatgcggc
     8401 cctacgacct gcaggacccc agaatccaca ccgtgctgag cggcgaggtg tatacatgca
     8461 tgtgcttcct gatcgatatg gtgaacgtga gcctggagct gaaggacccc aagaggaagg
     8521 agggagcagg ctccctggca cggttcgact tcaagaagtg caagctgctg tacgagtcct
     8581 tttctaatca gaccaagtcc atcaacctgg tgagccactc catgatggcc ttcgacacac
     8641 ggtatgccgg ccagaagacc tccccaggca tgacaaacgt gttctcttgc atctttcagc
     8701 ccgccaagaa ttctagcacc acacagggct ccatccagat cgagctgcac ttccgctcta
     8761 ccaaggactc ctcttgtttt acagtggtgc tgaacaatct gcgggtgttc ctgatctttg
     8821 actggctgct gctggtgcac gatttcctgc acaccccaag cgacatcaag aagcagaatc
     8881 acgtgacacc cagcagacac aggaacagct cctctgagtc cgccatcgtg ccaaagaccg
     8941 tgaagtctgg cgtggtgaca aagagaagct ccctgcccgt gtccaatgag aggcacctgg
     9001 aggtgaaggt gaacgtgaca ggcaccgagt tcgtggtcat cgaggacgtg agctgctttg
     9061 ataccaacgc catcatcctg aagggcacca cagtgctgac atacaagcca agattcgtgg
     9121 acaggccctt ttctggcagc ctgttcggca tcgaggtgtt tagctgtaga ctgggcaacg
     9181 agcacgatac cgccctgtcc atcgtggacc ctgtgcagat ccagatggag ctggtgggca
     9241 attctagcta ccagaactcc tctggcctga tggacgcctt caattccgag gattttcctc
     9301 cagtgctgga gatccagctg caggccctgg atatcaggct gtcctataac gacgtgcagc
     9361 tgttcctggc catcgccaag tctatccctg agcaggccaa tgcagcagtg ccagatagcg
     9421 tggccctgga gtccgactct gtgggcacct acctgccagg agcatcccgc gtgggagagg
     9481 agatcaggga gggaacaagg cacaccctgg accccgtgct ggagctgcag ctggcaagac
     9541 tgcaggagct gggcttttcc atggacgatt gcaggaaggc cctgctggca tgtcagggac
     9601 agctgaagaa ggccgccagc tggctgttca agaacgcaga gcccctgaag tccctgtccc
     9661 tggcatctac cagcagggac agccctggag cagtggcagc accactgatc agcggcgtgg
     9721 agatcaaggc cgagtccgtg tgcatctgtt tcatcgacga ttgcatggac tgtgatgtgc
     9781 ctctggccga gctgacattc tccaggctga actttctgca gcgcgtgcgg acctctccag
     9841 agggatacgc ccactttaca ctgagcggcg actactataa tcgcgccctg tccggctggg
     9901 agccattcat cgagccctgg ccttgctccg tgtcttggca gcagcaggca gcatccagac
     9961 tgcacccacc taggctgaag ctggaggcaa aggcaaagcc acgcctggat atcaacatca
    10021 ccagcgtgct gatcgaccag tacgtgagca caaaggagtc ctggatggcc gattattgca
    10081 aggacgataa ggacatcgag tccgccaagt ctgaggattg gatgggcagc tccgtggacc
    10141 caccctgttt cggccagagc ctgcccctgg tgtacctgag aacaaggagc accgcctccc
    10201 tgaccaacct ggagcaccag atctatgccc gcgccgaggt gaagacacct aagaggcgcc
    10261 agcccttcgt gccttttgcc ctgcggaatc acacaggctg taccctgtgg tttgccaccc
    10321 tgaccacaac cccaacaaga gccgccctga gccactccgg ctctcccgga gtggtgcctg
    10381 agggaaatgg caccttcctg gacgatacac acaacgtgtc cgagtggagg gaggtgctga
    10441 ccggcgagga gatccccttc gagtttgagg caaggggcaa gctgaggcac aggcacacac
    10501 acgacctgag aatccaccag ctgcaggtga gggtgaacgg ctgggagcag gtgtctcctg
    10561 tgagcgtgga taaagtgggc accttctttc ggtacgccgc cccagacaag aactctagct
    10621 cctctaccat cggcagcccc agctccagaa caaatatcat ccaccctcag gtgtattttt
    10681 ctagcctgcc tccagtgagg gtggtgttcg ccgtgaccat ggagggctct gccagaaaag
    10741 tgatcacagt gaggagcgcc ctgatcgtga gaaaccggct ggagacaccc atggagctga
    10801 gactggacag cccatccgcc cccgataagc ctgtggtgct gccagccatc atgcccggcg
    10861 attcctttgc cgtgcctctg cacctgacat cttggagact gcaggccagg cctaagggcc
    10921 tgggcgtgtt cttttgcaag gccccaatcc actggaccaa tgtggtgaag acagccgaga
    10981 tctcctctag caagagggag tgccacagca tggacactga gaagtcccgc ttctttcggt
    11041 tctgcgtggc catcaagaag gagaactacc ctgattatat gccaagcaat atcttctctg
    11101 acagcgccaa gcagatcttt cgccagcccg gccacaccat ctacctgctg cctacagtgg
    11161 tcatctgcaa cctgctgccc tgtgagctgg acttctacgt gaagggcatg cccatcaatg
    11221 gcaccctgaa gcctggcaag gaggccgccc tgcacacagc agacacctct cagaatatcg
    11281 agctgggcgt gagcctggag aacttccctc tgtgcaagga gctgctgatc ccccctggca
    11341 cccagaacta catggtgcgc atgcggctgt atgacgtgaa tcggagacag ctgaacctga
    11401 caatcaggat cgtgtgcagg gcagagggct ctctgaagat cttcatcagc gccccttact
    11461 ggctgatcaa caagaccggc ctgccactga tctttaggca ggacaatgcc aagacagatg
    11521 cagcaggaca gttcgaggag cacgagctgg cacgctccct gtctcccctg ctgttttgct
    11581 atgccgataa ggagcagcct aacctgtgca ccatgagaat cggaaggggc atccacccag
    11641 agggaatgcc tggatggtgc cagggcttct ccctggacgg aggctctggc gtgcgggccc
    11701 tgaaagtgat ccagcagggc aataggcctg gcctgatcta caacatcggc atcgatgtga
    11761 agaagggcag aggcaggtac atcgacacct gtatggtcat ctttgcccca cggtatctgc
    11821 tggacaacaa gtcctctcac aagctggcct tcgcccagag agagtttgca aggggacagg
    11881 gaaccgcaaa tccagagggc tacatcagca cactgccagg cagctccgtg gtgttccact
    11941 ggccccgcaa cgactatgat cagctgctgt gcgtgcggct gatggatgtg cctaattgta
    12001 tctggagcgg cggcttcgag gtgaacaaga acaattcctt tcacatcaat atgagagaca
    12061 ccctgggcaa gtgcttcttt ctgagggtgg agatcaccct gaggggagca acataccgga
    12121 tcagcttttc cgacaccgat cagctgccac cccctttcag gatcgacaac ttttccaagg
    12181 tgcctgtggt gttcacacag cacggagtgg cagagcctag gctgaggacc gaggtgaagc
    12241 caatgacaag cctggactat gcctgggatg agccaaccct gccacccttt atcacactga
    12301 ccgtgaaggg cgccggctct agcgagatca actgcaatat gaacgacttc caggataatc
    12361 gccagctgta ctatgagaac ttcatctaca tcgccgccac atataccttt tccggcctgc
    12421 aggagggaac cggccggccc gtggcctcta acaaggccat cacatgtgcc gagctggtgc
    12481 tggacgtgag ccctaagacc cagagagtga tcctgaagaa gaaggagcca ggcaagagat
    12541 ctcagctgtg gaggatgaca ggcaccggca tgctggcaca cgagggctcc tctgtgccac
    12601 acaatcccaa caagccttct gccgcacgga gcaccgaggg ctccgccatc ctggatatcg
    12661 caggcctggc agcagtgaca gacaatcgct acgagcccct gatgctgagg aagcctgaca
    12721 ggcgccggtc cacaacccag acctggtctt ttagagaggg caagctgaca tgcggcctgc
    12781 acggcctggt ggtgcaggca aagggaggcc tgtccggcct gttcgatgga gcagaggtgg
    12841 tgctgggacc agacacctct atggagctgc tgggccccgt gcctccagag cagcagttta
    12901 tcaaccagaa gatgcggcca ggctctggca tgctgagcat ccgcgtgatc ccagatggac
    12961 caacccgggc cctgcagatc acagacttct gtcaccgcaa gagctcccgg tcctaatgag
    13021 gtggatgaga gctcccggtc ctaatgaggt ggatgagctg cccgtgaccg agcaggagct
    13081 gcagaagctg aagaatcctg acacagagca ggagctggag gtgctggtga gactggaggg
    13141 cggcattggc ctgtccctga tcaacaaggt gcccgaggag ctggtgtttg cctctctgac
    13201 cggcatcaat gtgcactaca cccagctggc cacaagccac atgctggagc tgtccatcca
    13261 ggacgtgcag gtggataacc agctgatcgg cacaacccag ccattcatgc tgtatgtgac
    13321 ccccctgagc aatgagaacg aagtgatcga gacaggccca gcagtgcagg tgaacgcagt
    13381 gaagtttcct tctaagagcg ccctgaccaa tatctacaag cacctgatga tcaccgccca
    13441 gcggttcaca gtgcagatcg aggagaagct gctgctgaag ctgctgagct tctttggcta
    13501 cgatcaggcc gagtccgagg tggagaagta tgacgagaat ctgcacgaga agaccgcaga
    13561 gcagggagga acacctatca gatactattt tgagaacctg aagatctcca tcccacagat
    13621 caagctgtcc gtgttcacct ctaataagct gcccctggat ctgaaggccc tgaagagcac
    13681 actgggcttc cctctgatca gatttgagga cgccgtgatc aacctggacc ccttcaccag
    13741 ggtgcacccc tacgagacaa aggagttcat catcaatgac atcctgaagc actttcagga
    13801 ggagctgctg tcccaggccg ccagaatcct gggctctgtg gatttcctgg gcaatcctat
    13861 gggcctgctg aacgacgtgt ccgagggcgt gaccggcctg atcaagtatg gcaatgtggg
    13921 cggcctgatc cggaacgtga cacacggcgt gtccaattct gccgcaaagt tcgcaggcac
    13981 cctgagcgat ggcctgggca agacaatgga caacagacac cagagcgaga gagagtacat
    14041 caggtatcac gcagccacct ccggagagca cctggtggcc ggcatccacg gcctggcaca
    14101 cggaatcatc ggaggcctga ccagcgtgat cacatccacc gtggagggag tgaagacaga
    14161 gggaggcgtg tctggcttta tcagcggcct gggcaagggc ctggtgggaa cagtgaccaa
    14221 gcccgtggca ggcgccctgg atttcgcaag cgagacagca caggccgtgc gcgacacagc
    14281 caccctgtcc ggaccaagga cccaggcaca gagagtgagg aagccaaggt gctgtacagg
    14341 cccacagggc ctgctgccaa ggtacagcga gtcccaggca gagggacagg agcagctgtt
    14401 caagctgacc gacaacatcc aggatgagtt ctttatcgcc gtggagaata tcgatagcta
    14461 ttgcgtgctg atctctagca aggccgtgta ctttctgaaa tccggcgact atgtggatcg
    14521 ggaggccatc ttcctggagg tgaagtacga cgatctgtat cactgtctgg tgtccaagga
    14581 ccacggcaag gtgtacgtgc aggtgaccaa gaaggccgtg tctacatcct ctggcgtgag
    14641 catcccagga ccatcccacc agaagcccat ggtgcacgtg aagagcgagg tgctggccgt
    14701 gaagctgtcc caggagatca actatgccaa gtctctgtac tatgaacagc agctgatgct
    14761 gagactgagc gaaaacaggg aacagctgga actggattca taaaccggtt agtaatgagt
    14821 ttgatatctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    14881 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    14941 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    15001 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    15061 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    15121 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    15181 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
    15241 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    15301 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    15361 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    15421 gcctggaaac gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    15481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
    15541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    15601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    15661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg
    15721 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    15781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    15841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc
    15901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    15961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    16021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    16081 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct
    16141 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
    16201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    16261 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    16321 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    16381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    16441 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    16501 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
    16561 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
    16621 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
    16681 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
    16741 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
    16801 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
    16861 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
    16921 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
    16981 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
    17041 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
    17101 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
    17161 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
    17221 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
    17281 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
    17341 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga
    17401 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
    17461 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
    17521 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
    17581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    17641 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    17701 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    17761 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    17821 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    17881 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    17941 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    18001 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    18061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    18121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    18181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    18241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    18301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    18361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    18421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    18481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    18541 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    18601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    18661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    18721 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    18781 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    18841 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    18901 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    18961 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    19021 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    19081 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    19141 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    19201 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    19261 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    19321 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    19381 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    19441 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    19501 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    19561 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    19621 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    19681 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    19741 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    19801 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    19861 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca
    19921 atctgctctg atgccgcata gttaagccag
//
