LOCUS       sequence-382426-       10364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Gata2 without the nuclear 
            localisation signal (amino acids 380-440), in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pFUW-tetO-mCh-Gata2-del-NLS(380-440)
FEATURES             Location/Qualifiers
     source          1..10364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..594
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             612..792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    839..964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1457..1690
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1875..1919
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2217..2334
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2391..2409,2410..2432,2433..2451,2452..2474,2475..2493,
                     2494..2516,2517..2535,2536..2558,2559..2577,2578..2600,
                     2601..2619,2620..2642,2643..2661)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2694..2732
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2729..2753
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2844..3545
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2977..2995)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3245..3264
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3439..3462
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4833..5421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4886..4906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5304..5315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5424..5440)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5425..5441)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5946..6126
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6152..6169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6158..6382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6428..6856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6515..6534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6725..6746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6865..6885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6870..7199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7050..7185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7112..7131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7247..7294
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7313..7687
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7817..7938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7854..7873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7908..7927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7987..8003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7987..8003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8000..8022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8011..8027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8035..8041,8042..8059,8060..8065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8080..8101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8218..8235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8389..8977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8469..8488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9148..9939,9940..10008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9771..9790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10009..10113)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10188..10207)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
      601 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      781 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      841 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      901 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      961 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     1021 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     1081 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     1141 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     1201 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     1261 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     1321 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1381 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1441 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1501 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1561 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1621 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1681 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1741 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1801 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1861 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1921 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1981 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     2041 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     2101 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     2161 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     2221 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2281 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     2341 tattacaggg acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag
     2401 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2461 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2521 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2581 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2641 actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc
     2701 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     2761 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg
     2821 gccccgaatt caccggttct agaatggtga gcaagggcga ggaggtcatc aaagagttca
     2881 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
     2941 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     3001 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
     3061 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
     3121 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
     3181 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
     3241 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     3301 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
     3361 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
     3421 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     3481 aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt
     3541 acaaggaggt ggcgcctgag cagcctcgct ggatggcgca ccccgccgta ttgaatgcgc
     3601 agcaccccga ctcgcaccat ccgggcctgg cgcataacta catggagcca gcacagctgc
     3661 tgcctcccga cgaggtggat gtcttcttca accatctcga ctcgcagggc aacccttact
     3721 acgccaaccc ggcccacgcg cgcgcgcgcg tttcctacag cccggcgcat gcccgtctca
     3781 ccggaggcca gatgtgccga ccacacttgt tgcacagccc aggcttgccg tggctggacg
     3841 ggggcaaagc agctctctct gccgccgctg cccatcacca cagtccctgg accgtcagcc
     3901 cgttctccaa gaccccgctg cacccctcag ctgctggagc acccggaggg cctctgtctg
     3961 tttacccagg ggctgcgggt gggagcgggg gaggcagtgg gagctccgtg gcctccctca
     4021 cccccactgc agcccactcg ggctcccatc tcttcggctt cccacccacg ccacccaaag
     4081 aagtgtctcc agaccccagc acaacaggag ctgcttcccc ggcctcttct tctgcagggg
     4141 gtagtgtagc ccggggtgag gacaaggatg gcgtcaagta ccaagtgtca ctctccgaga
     4201 gcatgaagat ggaaggcggc agtcccctgc gcccgggcct agctaccatg ggcacccagc
     4261 ctgcaacaca ccacccgata cccacctatc cctcctatgt gcccgccgca gctcatgact
     4321 atggcagcag tctcttccat ccaggaggct tcctgggtgg ccccgcctcc agcttcaccc
     4381 ctaagcagag aagcaaggct cgctcctgct cagaaggccg ggagtgtgtc aactgtggtg
     4441 ccacagccac ccctctctgg cgacgagatg gcacgggcca ctacctgtgc aatgcctgtg
     4501 ggctctacca caagatgaat ggacagaacc ggccgctcat caagcccaag cggaggctgt
     4561 ctgctgccag aagagcgggc acctgttgtg caaattgtca gacgacaacc accaccttat
     4621 ggcgccggaa cgccaacggg gaccctgtgt gcaacgcctg tggcctctac tacaagctgg
     4681 gacacctccc accttttagt cactctggac acatcctacc cacgcccacg cctatccacc
     4741 cttcctccag tctctctttt ggccaccccc acccgtccag catggtgact gccatgggct
     4801 aggctagctt cgaagatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     4861 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4921 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4981 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5041 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5101 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5161 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5221 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5281 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5341 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5401 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     5461 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5521 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     5581 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5641 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5701 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     5761 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     5821 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     5881 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     5941 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6001 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6061 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6121 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     6181 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6241 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6301 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6361 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     6421 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6841 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6901 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7021 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     7141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7201 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     7261 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     7321 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     7381 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     7441 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     7501 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     7561 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     7621 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     7681 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7741 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7801 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7921 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10141 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
    10201 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
    10261 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
    10321 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggc
//
