LOCUS       sequence-382586-        3467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SuperNova Green in the cytosol of mammalian cells, joined 
            by a T2A cleavage site with dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Sessler CD, Zhou Y, Wang W, Hartley ND, Fu Z, Graykowski D, Sheng M,
            Wang X, Liu J
  TITLE     Optogenetic polymerization and assembly of electrically functional 
            polymers for modulation of single-neuron excitability.
  JOURNAL   Sci Adv. 2022 Dec 9;8(49):eade1136. doi: 10.1126/sciadv.ade1136. 
            Epub 2022 Dec 7.
  PUBMED    36475786
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-12-9"; volume: "8(49):eade1136. doi"; pages: " 
            10.1126/sciadv.ade1136. Epub 2022 Dec 7"
COMMENT     Sequence Label: pAAV-CAG-SuperNovaGreen-T2A-dTomato
FEATURES             Location/Qualifiers
     source          1..3467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   131..260
                     /label=AAV2 ITR
     polyA_signal    422..543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(459..478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     513..532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    586..1174
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             692..703
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1101..1121
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1905..1958)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2784..2807)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2807..2816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2849..2868)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2930..2952)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gkmcwcscgc rssgammcca ccacmcccgc cgcgcttaak gcgccgctac agggcgcgtc
       61 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      121 attacgccag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
      181 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
      241 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta
      301 gccatgctct aggaagatcc ttatcgattt taccacattt gtagaggttt tacttgcttt
      361 aaaaaacctc ccacatctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      421 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      541 ttatcatgtc tgctcgaagc ggccgcccgg gtcgacggta tcgatgcggg gaggcggccc
      601 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
      661 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
      721 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag gacgatgatt
      781 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
      841 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
      901 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
      961 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     1021 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     1081 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     1141 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttactt atacagctca
     1201 tccatgccat acaagaacag gtgatgccga ccttctgagc gttcgtactg ctcgacgatt
     1261 gtgtagtcct cgttgtgact cgtgatgtcc aacttggtgt ccacgtagta gtagccaggc
     1321 agctgaaccg gctttttggc catatatata gttttgaact caaccaggta gtgcccgcca
     1381 tctttgagtt taagcgcctg atggatttcg cctttaagaa cgccatcgcg ggggtacagt
     1441 cgttctgtag aggcctccca ccccatggtc ttcttctgca tcacggggcc atcgggggga
     1501 aaatttgttc cgcgcatctt caccttgtaa atcagggtgc cgtcctgcag agaggagtct
     1561 tgcgtgacag tgaccagccc accatcctca aaattcatta ctcgttccca cttaaatcct
     1621 tcaggaaatg acagtttttt gtaatcaggt atatcagcag ggtgtttgac gtaagccttg
     1681 ctaccgtaca tgaattgagg gctgaggatg tcccaggcga agggcagagg tccgcccttg
     1741 gtcactttaa gcttggcggt ctgggtcccc tcataaggtc tgccttcgcc ctcgccttcg
     1801 atctcgaact catgtccgtt catagagcct tccatccgca ccttaaatct catgaactcc
     1861 ttgatcactt cctccccctt ggacaccatg gtgctgccag gagcgggtcc gggattctct
     1921 tccacgtcgc cacatgtcag cagagagcct ctgccttcgg acctgctcag cacgcagcag
     1981 ctcatacaac cagggccgct ctcatcagga gggttcagct tgcctccgcc gctgccctta
     2041 aggccgccag agtcctcgtc actgccgatg gcgctggtga ttcttggcac gctgtgggcg
     2101 taggcgactt cccgctggca cacgtgatct cttttatcgg atgtatctct tgtctgcttt
     2161 gtgataatgg tcacgaagtg aggtccaggg atctcgatgg ctctgctgcc gttaaaggtc
     2221 atcttgctgt cgaagtggcc catcattttt ccgccgtcgg ctgtggtgaa gccgatggtg
     2281 gcggtctggc gcacggcgtt tgggccatga gggaacatgt gggtctcgct aggcaggatg
     2341 tccaccagct ggtcccgcat aatggggccg tcaggttgaa agccgtcgca attcacggtg
     2401 atccggctga ccacgcaggt gtcgtccagc tcgtaggtgt ggtgagatgt catggtgccg
     2461 tcgttttcaa accgcacggt tctatcgatg ctcaggccct cggggaagca ctcctgggcg
     2521 aagtggctga ttccatcggg gtatctagcg aagaaaggct cgccccactg gatcaggtgg
     2581 cagataggct tccagctcat tggcagcttg cctgtttcac aaacggcgtg agcgttgaag
     2641 tcgccgtggg ggaacttgct ggagccatcg gccacgatgg taaacttctg gccgttcacc
     2701 tcgccgtcga tgaagatttt gaatgtcatg tcgctttgga acagggcagg gcccacctcg
     2761 ctgctgcctc cgctagcgcc gcccttatcg tcatcgtcct tgtaatccat ggtggcggat
     2821 ccaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta
     2881 ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc
     2941 agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc
     3001 cccgagccgc tccccccgca caaagggccc tcccggagcc cctcaaggct ttcacgcagc
     3061 cacagaaaag aaacaagccg tcattaaacc aagcgctaat tacagcccgg aggagaaggg
     3121 ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc agagccgggg cgggcggcgc
     3181 gaggcggcgg cggagcgggg cacggggcga aggcagcgcg cagcgactcc cgcccgccgc
     3241 gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg ccataaaagg aaactttcgg
     3301 agcgcgccgc tctgattggc tgccgccgca cctctccgcc tcgccccgcc ccgcccctcg
     3361 ccccsccccc ccccccccng gcgcgcgccc cccccccccc cccccccccc cccccccccc
     3421 aaacaaatta aaaaaaaaaa aaanaaaaaw targnngggg ggggggg
//
