LOCUS       sequence-382632-        5151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control plasmid that expresses miniSOG targeted to the cell membrane
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Sessler CD, Zhou Y, Wang W, Hartley ND, Fu Z, Graykowski D, Sheng M,
            Wang X, Liu J
  TITLE     Optogenetic polymerization and assembly of electrically functional 
            polymers for modulation of single-neuron excitability.
  JOURNAL   Sci Adv. 2022 Dec 9;8(49):eade1136. doi: 10.1126/sciadv.ade1136. 
            Epub 2022 Dec 7.
  PUBMED    36475786
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-12-9"; volume: "8(49):eade1136. doi"; pages: " 
            10.1126/sciadv.ade1136. Epub 2022 Dec 7"
COMMENT     Sequence Label: pAAV-CAG-miniSOG-C-Far
FEATURES             Location/Qualifiers
     source          1..5151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   56..185
                     /label=AAV2 ITR
     primer_bind     1054..1076
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1138..1157
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1199..1222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1238..1555
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     misc_feature    1650..2238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1703..1723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2121..2132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2281..2402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2292..2311)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2346..2365
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2564..2693
                     /label=AAV2 ITR
     rep_origin      2765..3220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2852..2871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3062..3083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3246..3350
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3351..3419,3420..4211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3569..3588)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4382..4970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4871..4890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatcgtacca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      481 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      541 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      601 ggcggggggg gggggggggc gcgcgccrgg sggggsgggg sggggsgrgg ggsggggsgg
      661 ggsgrggsgg rgaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      721 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      781 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
      841 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      901 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
      961 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggctgt ccgcgggggg
     1021 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1081 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1141 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attggatccg ccaccatgga
     1201 ttacaaggat gacgacgata agggcggagg tggttctatg gagaaaagtt tcgtgataac
     1261 tgatccacgg ctgccagaca atcccatcat cttcgcatcc gatggcttcc tggagctgac
     1321 cgagtattcc agagaggaga tcctgggccg caatggccgc tttctgcagg gaccagagac
     1381 agaccaggcc acagtgcaga agattcgcga tgccattaga gatcagcgcg agattaccgt
     1441 gcagctgata aactacacaa aaagcgggaa gaaattctgg aacctcctgc acctccagcc
     1501 catgagggac cagaagggtg agctccagta tttcatcgga gtgcagctgg atggatcagg
     1561 aggaggctca ggaggaggaa agctgaaccc tcctgatgag agtggccccg gctgcatgag
     1621 ctgctgtgtg ctctcctaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     1681 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1741 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1801 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1861 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1921 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     1981 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2041 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     2101 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2161 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2221 tttgggccgc ctccccgcat cgataccgtc gacccgggcg gccgcttcga gcagacatga
     2281 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2341 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2401 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     2461 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt cctagagcat
     2521 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     2581 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     2641 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctggcgt
     2701 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2761 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2821 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2881 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2941 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3001 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3061 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3121 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3181 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     3241 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3301 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3361 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3421 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3481 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3541 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3601 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3661 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3721 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3781 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3841 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3901 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3961 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4021 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4081 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4141 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4201 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4501 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5041 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
