LOCUS       sequence-382712-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian
            cells with localization in the nucleus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Martin A, Rivera-Fuentes P
  TITLE     A general strategy to develop fluorogenic polymethine dyes for 
            bioimaging.
  JOURNAL   Nat Chem. 2024 Jan;16(1):28-35. doi: 10.1038/s41557-023-01367-y. 
            Epub 2023 Nov 27.
  PUBMED    38012391
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-023-01367-y"; journalName: "Nat Chem"; date: 
            "2024-01"; volume: "16"; issue: "1"; pages: "28-35"
COMMENT     Sequence Label: H2B-SNAPf-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     CDS             430..975
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
       61 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
      121 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
      181 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
      241 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
      301 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag
      361 tacaccagcg ctaaggctag cgatatcggc gcgccagcat ttaaatctgt acagaccggt
      421 gaattcacca tggacaaaga ctgcgaaatg aagcgcacca ccctggatag ccctctgggc
      481 aagctggaac tgtctgggtg cgaacagggc ctgcaccgta tcatcttcct gggcaaagga
      541 acatctgccg ccgacgccgt ggaagtgcct gccccagccg ccgtgctggg cggaccagag
      601 ccactgatgc aggccaccgc ctggctcaac gcctactttc accagcctga ggccatcgag
      661 gagttccctg tgccagccct gcaccaccca gtgttccagc aggagagctt tacccgccag
      721 gtgctgtgga aactgctgaa agtggtgaag ttcggagagg tcatcagcta cagccacctg
      781 gccgccctgg ccggcaatcc cgccgccacc gccgccgtga aaaccgccct gagcggaaat
      841 cccgtgccca ttctgatccc ctgccaccgg gtggtgcagg gcgacctgga cgtggggggc
      901 tacgagggcg ggctcgccgt gaaagagtgg ctgctggccc acgagggcca cagactgggc
      961 aagcctgggc tgggt
//
