LOCUS       sequence-382812-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged human Nix with an alanine 
            substitution of serine-35. Based on Myc-Nix (#100795).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Field JT, Chapman D, Hai Y, Ghavami S, West AR, Ozerklig B, Saleem 
            A, Kline J, Mendelson AA, Kindrachuk J, Triggs-Raine B, Gordon JW
  TITLE     The mitophagy receptor BNIP3L/Nix coordinates nuclear calcium 
            signaling to modulate the muscle phenotype.
  JOURNAL   Autophagy. 2025 Jul;21(7):1544-1555. doi: 
            10.1080/15548627.2025.2476872. Epub 2025 Mar 24.
  PUBMED    40063005
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2025.2476872"; journalName: "Autophagy"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1544-1555"
COMMENT     Sequence Label: Myc-Nix-S35A
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(735..753)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(736..753)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(773..790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    779..988
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctgatctc cgaggaggac ctgggatcca tcgatgtcga cgaattcatg tcgtcccacc
       61 tagtcgagcc gccgccgccc ctgcacaaca acaacaacaa ctgcgaggaa aatgagcagt
      121 ctctgccccc gccggccggc ctcaacagtg cctgggtgga gctacccatg aacagcagca
      181 atggcaatga taatggcaat gggaaaaatg gggggctgga acacgtacca tcctcatcct
      241 ccatccacaa tggagacatg gagaagattc ttttggatgc acaacatgaa tcaggacaga
      301 gtagttccag aggcagttct cactgtgaca gcccttcgcc acaagaagat gggcagatca
      361 tgtttgatgt ggaaatgcac accagcaggg accatagctc tcagtcagaa gaagaagttg
      421 tagaaggaga gaaggaagtc gaggctttga agaaaagtgc ggactgggta tcagactggt
      481 ccagtagacc cgaaaacatt ccacccaagg agttccactt cagacaccct aaacgttctg
      541 tgtctttaag catgaggaaa agtggagcca tgaagaaagg gggtattttc tccgcagaat
      601 ttctgaaggt gttcattcca tctctcttcc tttctcatgt tttggctttg gggctaggca
      661 tctatattgg aaagcgactg agcacaccct ctgccagcac ctactgactc gagcatgcat
      721 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
      781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      901 agtaggtgtc attctattct gggggggtgg gggtggggca ggacagcaag ggggaggatt
      961 gggaagacaa tagcaggcat gctggggatg cg
//
