LOCUS       sequence-382815-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged human Nix with an aspartic acid 
            substitution of serine-35. Based on Myc-Nix (#100795).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Field JT, Chapman D, Hai Y, Ghavami S, West AR, Ozerklig B, Saleem 
            A, Kline J, Mendelson AA, Kindrachuk J, Triggs-Raine B, Gordon JW
  TITLE     The mitophagy receptor BNIP3L/Nix coordinates nuclear calcium 
            signaling to modulate the muscle phenotype.
  JOURNAL   Autophagy. 2025 Jul;21(7):1544-1555. doi: 
            10.1080/15548627.2025.2476872. Epub 2025 Mar 24.
  PUBMED    40063005
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2025.2476872"; journalName: "Autophagy"; date: 
            "2025-07"; volume: "21"; issue: "7"; pages: "1544-1555"
COMMENT     Sequence Label: Myc-Nix-S35D
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(737..755)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(738..755)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(775..792)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    781..1006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agagctgatc tccgaggagg acctgggatc catcgatgtc gacgaattca tgtcgtccca
       61 cctagtcgag ccgccgccgc ccctgcacaa caacaacaac aactgcgagg aaaatgagca
      121 gtctctgccc ccgccggccg gcctcaacag tgactgggtg gagctaccca tgaacagcag
      181 caatggcaat gataatggca atgggaaaaa tggggggctg gaacacgtac catcctcatc
      241 ctccatccac aatggagaca tggagaagat tcttttggat gcacaacatg aatcaggaca
      301 gagtagttcc agaggcagtt ctcactgtga cagcccttcg ccacaagaag atgggcagat
      361 catgtttgat gtggaaatgc acaccagcag ggaccatagc tctcagtcag aagaagaagt
      421 tgtagaagga gagaaggaag tcgaggcttt gaagaaaagt gcggactggg tatcagactg
      481 gtccagtaga cccgaaaaca ttccacccaa ggagttccac ttcagacacc ctaaacgttc
      541 tgtgtcttta agcatgagga aaagtggagc catgaagaaa gggggtattt tctccgcaga
      601 atttctgaag gtgttcattc catctctctt cctttctcat gttttggctt tggggctagg
      661 catctatatt ggaaagcgac tgagcacacc ctctgccagc acctactgac tcgagcatgc
      721 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
      781 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      841 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      901 tgagtaggtg tcattctatt ctgggggggt ggggtggggc aggacagcaa gggggaggan
      961 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
//
