LOCUS       sequence-38304-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, 
            Gumper I, Sabatini DD, De Robertis EM
  TITLE     Wnt signaling requires sequestration of glycogen synthase kinase 3 
            inside multivesicular endosomes.
  JOURNAL   Cell. 2010 Dec 23. 143(7):1136-48.
  PUBMED    21183076
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Dec 23. 143(7):1136-48."
COMMENT     Sequence Label: pCS2 stabilized mutant Beta Catenin-GFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..763)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(90..110)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     705..733
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcactatag ttctagaggc tcgagaggcc ttgaattccg gaattcttat ttgtatagtt
       61 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      121 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      181 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      241 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
      301 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt
      361 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      421 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      481 cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatctg
      541 ggtatcttga aaagcattga acaccataag tgagagtagt gacaagtgtt ggccatggaa
      601 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      661 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
      721 gaattgggac aactccagtg aaaagttctt ctcctttact catcaatcga tacaagtcag
      781 tgtcaaacca ggccagttgg ttgctatcac ctggaggaag tccatccata agatcttggg
      841 catgactcaa atcaggaagt ccatcanctg gatagtctgc
//
