LOCUS       sequence-383131-       10272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding a human codon-optimized dCas12Max-CBE driven by 
            CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and 
            mCherry driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang 
            W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, 
            Li J, Zhong N, Zhang M, Zhou Y, Yang H
  TITLE     An engineered xCas12i with high activity, high specificity, and 
            broad PAM range.
  JOURNAL   Protein Cell. 2023 Jun 28;14(7):538-543. doi: 
            10.1093/procel/pwac052.
  PUBMED    37378659
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Protein Cell"; date: "2023-06-28- 28"; volume: "14"; issue: "7"; 
            pages: "538-543"
COMMENT     Sequence Label: dCas12Max-CBE
FEATURES             Location/Qualifiers
     source          1..10272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        299..374
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        471..756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        758..1035
                     /label=chicken beta-actin promoter
     intron          1036..1263
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1317..1337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1992..2012
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5283..5303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5316..5564
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5619..5639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5667..5684)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5673..5880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5949..6252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6253..6456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6407..6427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6485..7195
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6630..6648)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     rep_origin      7750..8205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7837..7856)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8047..8068
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8222..8241)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8341..8363
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8401..8419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8487..8591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8592..8660,8661..9452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8810..8829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9623..10211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10112..10131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agaaatgtgt ccccagttga cacagagacc gagaggtctc gtttttttgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      361 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
      421 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttacataa
      481 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      541 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      601 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      661 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
      721 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      781 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      841 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
      901 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
      961 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     1021 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     1081 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     1141 gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg
     1201 ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt
     1261 caggttggac cggtgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa
     1321 agaagaagcg gaaagtcatg gaggcatctc cagcaagcgg accaaggcac ctgatggacc
     1381 cccacatctt cacctctaac tttaacaatg gcatcggcag gcacaagaca tacctgtgct
     1441 atgaggtgga gcgcctggac aatggcacca gcgtgaagat ggatcagcac agaggcttcc
     1501 tgcacaacca ggccaagaat ctgctgtgcg gcttctacgg ccggcacgca gagctgagat
     1561 ttctggacct ggtgcctagc ctgcagctgg atccagccca gatctatagg gtgacctggt
     1621 tcatcagcta ctccccatgc ttttccgcgg gatgtgcagg agaggtgcgc gccttcctgc
     1681 aggagaacac acacgtgcgg ctgagaatct ttgccgcccg gatcttcgac tatgatcctc
     1741 tgtacaagga ggccctgcag atgctgagag acgcaggagc ccaggtgtcc atcatgacct
     1801 atgatgagtt caagcactgc tgggacacat ttgtggatca ccagggctgt ccctttcagc
     1861 cttgggacgg actggatgag cactcccagg ccctgtctgg caggctgagg gccatcctgc
     1921 agaaccaggg caattccgga ggatctagcg gaggctccaa gagaaccgct gatggctcag
     1981 agtttgagag cccaaaaaag aaaagaaaag tctctggcgg aagctccggg ggctctatga
     2041 gttctgatgt ggtgcggcct tataacacaa agctgctccc agataacaga aagcacaata
     2101 tgttcctgca gaccttcaag cggctgaaca gcatctctct gaaccacttc gacctgctga
     2161 tctgcctgta cgctgcaatc accaacaaga aggccgagga atacaagtct gaaaaggaag
     2221 cccacgtgac cgccgatagc ctgtgtgcca tcaattggtt cagacccatg agcaagagat
     2281 acagcaaata cgccaccacc accttcaaca tgttagaact gtttaaggag tacagcggcc
     2341 acgagcctga tgcctattcc aagaactacc tgatgagcaa tatcgacagc gacagattcg
     2401 tgtgggtgga ttgtaggaag ttcgctaagg actttgccta tcagatggaa ctgggtttcc
     2461 acgagttcac cgtgttggcc gaaaccctgc tggctaattc tatcctggtg ctgaacgaga
     2521 gcaccaaggc caattgggct tggggaaccg tgtctgccct gtacggcggc ggagataagg
     2581 aggacagcac actgaagagc aagattctgc tggccttcgt ggacgccctg aacaaccacg
     2641 agctgaaaac aaagagagaa atcttgaatc aagtgtgtga atctctgaaa taccagagct
     2701 accaggacat gtacgtggat tttagaagcg tggttgacga aaacggcaac aagaagtctc
     2761 ctagaggctc tatgcctatc gtgaccaagt tcgagacaga cgacctgatc agcgacaacc
     2821 aaagaaaggc catgatcagc aacttcacta agaacgccgc tgccaaggca gctaagaaac
     2881 ctatccctta cttggaccgc ctgaaggagc acatggtgtc cctgtgcgac gagtacaatg
     2941 tgtatgcctg ggccgcggcc atcacaaaca gcaacgccga cgtgaccgcc cggaatacca
     3001 gaaacctgac attcatcggc gaacagaaca gcagacgaaa gagactgagc gtgctgcaga
     3061 caacaaccaa cgagaaggct aaggacatcc tgaacaagat caacgacaac ctgattcagg
     3121 aggtgcggta cacccctgcc cctaagcacc tgggcagaga tctggccaac ctgtttgata
     3181 cactgaagga aaaggacatc aacaacatcg agaacgaaga agagaaacag aacgtgatca
     3241 atgactgtat cgagcagtac gtggacgatt gcagaagcct caaccggaac cccatcgcag
     3301 ccctcctgaa gcacatctct aggtactacg aggatttcag cgccaagaat ttcctggacg
     3361 gcgccaagct gaacgtgctg actgaggtgg tgaaccggca gaaggcccac cccaccatct
     3421 ggagcgagaa ggcttacacc tggatcagca agttcgacaa gaaccggaga caggccaaca
     3481 gcagcctggt cggatgggtt gtgccccccg aggaggtgca caaggagaaa atcgccggac
     3541 agcagagcat gatgtgggtg accctcaccc tgctggacga cggcaagtgg gtcaaacatc
     3601 acatcccctt cagcgacagc agatactaca gcgaagtgta cgcctacaac cctaatctgc
     3661 cttatctgga cggaggcatc ccaagacaga gcaagttcgg caacaaacca acaaccaacc
     3721 tgacagccga gtcccaggcc ctcctggcta attctaagta caagaaagcc aacaagagct
     3781 tcctgcgggc taaagagaat gccacacaca acgtgcgggt gtcccctaac acctctctgt
     3841 gcattagact gctgaaggac agcgccggaa accagatgtt cgacaaaatc ggcaacgtgc
     3901 tcttcggcat gcagatcaac cacaagatca ccgtgggaaa acctaactac aagatcgagg
     3961 tgggcgacag attcctgggc ttcgatcaga accagagcga gaaccacacc tacgccgtgc
     4021 tgcagagagt gtccgagagc agtcacgaca cccaccactt taacggctgg gacgtgaagg
     4081 tgctggaaaa gggcaaagtg accagcgatg tgatcgtgcg ggacgaggtc tacgaccaac
     4141 tgtcttacga gggcgtcccc tacgatagca gcaagttcgc cgagtggcgg gacaagcgca
     4201 gaagatttgt gcttgagaac ctgagcatcc agctggaaga gggcaagacc ttcctgacag
     4261 agttcgacaa gctgaataag gacagcctgt accgctggaa catgaactac ctgaaactgc
     4321 tgagaaaggc catccgggcc ggaggcaaag agttcgccaa gatcgctaag acagagatct
     4381 tcgagctggc ggtggaaaga ttcggcccta ttaacctggg cagcctgtcc cagatcagcc
     4441 ttaagatgat tgcctccttt aagggcgtgg tccagtccta cttctccgtg agcggctgcg
     4501 tggatgatgc ctccaaaaag gcccatgatt ctatgctgtt cacatttatg tgcgccgccg
     4561 aagaaaagcg gaccaacaag agagaagaaa agaccaacag agccgccagc tttatcctgc
     4621 aaaaagccta cctgcatggc tgcaagatga tcgtgtgcga ggacgacctt cctgtggccg
     4681 acggcaagac aggcaaagcc cagaatgccg accggatgga ctggtgcgcc agagccctgg
     4741 ccaagaaggt gaacgacggc tgtgttgcca tgagcatctg ctacagagct atccctgcct
     4801 acatgagcag ccaccaggac ccctttgtgc acatgcagga taagaaaacc agcgtgctgc
     4861 ggcctagatt catggaagtt aataaggata gcatcagaga ctaccacgtg gcgggcctga
     4921 gaagaatgct gaacagcaag agtgacgctg gcaccagtgt ttattaccgg caagctgccc
     4981 tgcatttctg cgaagccctg ggcgtgagcc ctgaactggt gaaaaacaag aaaacccacg
     5041 ccgccgaact gggcaagcac atgggcagcg ctatgctgat gccctggaga ggcggtagag
     5101 tgtacatcgc cagcaaaaag ctgacctccg atgccaaatc agtgaagtac tgcggcgagg
     5161 atatgtggca gtaccacgcc gccgagatcg ccgctgttaa catcgccatg tatgaggtgt
     5221 gctgccagac cggcgctttc ggaaagaaac agaaaaaatc ggacgagctg cctggaggca
     5281 gccccaagaa gaagcggaag gtgtctggtg gttctaccaa cctgtctgac atcatcgaga
     5341 aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgcca gaggaggtgg
     5401 aggaagtgat cggcaacaag cccgagtccg atatcctggt gcacaccgcc tacgacgaga
     5461 gcacagatga gaatgtgatg ctgctgacct ccgacgcccc cgagtataag ccttgggccc
     5521 tggtcatcca ggattctaac ggcgagaata agatcaagat gctgtctggt ggttcttctg
     5581 gcggctcaaa gagaaccgcc gacggcagcg aatccgagcc caagaagaag aggaaagtct
     5641 aagaattcct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5701 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5761 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5821 tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga
     5881 gcggccgcta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5941 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6001 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6061 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6121 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6181 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6241 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     6301 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6361 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     6421 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
     6481 tagcatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     6541 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     6601 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     6661 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     6721 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     6781 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     6841 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     6901 cgtaatgcag aagaaaacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     6961 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     7021 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     7081 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     7141 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaggatc
     7201 cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
     7261 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
     7321 tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt tactgaaggc
     7381 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
     7441 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
     7501 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
     7561 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
     7621 tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgacct gcaggggcgc
     7681 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     7741 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7801 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     7861 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7921 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     7981 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8041 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     8101 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     8161 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     8221 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     8281 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8341 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     8401 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9301 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10261 ttgctcacat gt
//
