LOCUS       sequence-383202-        5985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green fluorescent, negative response-polarity voltage indicator 
            under the control of synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin 
            JA, Schnitzer MJ, Pieribone VA
  TITLE     Dual-polarity voltage imaging of the concurrent dynamics of multiple
            neuron types.
  JOURNAL   Science. 2022 Nov 4;378(6619):eabm8797. doi: 
            10.1126/science.abm8797. Epub 2022 Nov 4.
  PUBMED    36378956
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-11-4"; volume: "378(6619):eabm8797. doi"; pages: " 
            10.1126/science.abm8797. Epub 2022 Nov 4"
COMMENT     Sequence Label: pAAV-Syn-Ace-mNeon2
FEATURES             Location/Qualifiers
     source          1..5985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      642..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1551..1560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2112..2700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2165..2185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2583..2594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2732..3208
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2931..2950)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3248..3388
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3248..3377
                     /label=AAV2 ITR
     rep_origin      3463..3918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3550..3569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3760..3781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3935..3954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4054..4076
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4114..4132)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4200..4304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4305..4373,4374..5165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4523..4542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5336..5924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5825..5844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgccaccatg gctgacgtgg
      661 aaaccgagac cggcatgatt gcacagtgga ttgtctttgc tattatggct gctgctgcta
      721 ttgcttttgg agtggctgtg cactttcggc cttcagagct gaagagcgca tactatatca
      781 acattgccat ctgcactatc gccgctaccg cttactatgc aatggccgtg aactaccagg
      841 acctgacaat gaatggtgaa aggcaggtgg tctacgcaag atatatttcc tgggtgctga
      901 ccacaccact gctcctgctc gatctcatcg tcatgaccaa gatgggcgga gtgatgattt
      961 cttgggtcat cggcgcagac attttcatga tcgtgtttgg tattctgggc gccttcgagg
     1021 atgaacacaa gttcaaatgg gtgtacttta tcgctggatg tgtgatgcag gcagtcctga
     1081 catacgggat gtataacgcc acttggaaag acgatctgaa gaaaagcccc gagtaccata
     1141 gctcctatgt cagtctgctc gtcttcctgt caatcctctg ggtgttttat cctgtcgtgt
     1201 gggctttcgg gtctggtagt ggcgtgctgt ccgtcgacaa tgaggccatt ctcatgggaa
     1261 tcctggatgt gctcgctaag ccactgtttg gaatggggtg cctcattgcc catgagacta
     1321 tcttcaagtc ctacccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg
     1381 actttgacat ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc
     1441 tgaagtccac caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt
     1501 atggcttcca tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg
     1561 tagatggctc cggataccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta
     1621 ctgttaacta ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg
     1681 ggactggttt ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt
     1741 gcaggtcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt
     1801 acaccactgg aaatggcaag cgctacagga gcactgcgcg gaccacctac acctttgcca
     1861 agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc
     1921 tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga
     1981 tgggcatgga cgagctgtac aagaagagca ggatcaccag cgagggcgag tacatccccc
     2041 tggaccagat cgacatcaac gtgttctgct acgagaacga ggtgtaagaa ttcgatatca
     2101 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2161 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2221 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2281 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2341 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2401 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2461 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2521 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2581 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2641 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2701 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     2761 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     2821 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     2881 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     2941 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     3001 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     3061 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     3121 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     3181 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     3241 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3301 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3361 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     3421 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     3541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3661 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3781 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     3841 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3901 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3961 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4021 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4081 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4141 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4321 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4381 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4441 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4501 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4561 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4621 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4681 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4741 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4801 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4861 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4921 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4981 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5041 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5101 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5161 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
