LOCUS       sequence-38351-e        6599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pGV13 (CK2alpha K68M, CK2beta)
FEATURES             Location/Qualifiers
     source          1..6599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(136..154,155..177,178..196,197..219,220..238,239..261,
                     262..280,281..303,304..322,323..345,346..364,365..387,
                     388..406)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        438..475
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     640..656
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     640..656
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             673..702
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          1427..1999
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2131..2150)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2196..2251
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2250..2269)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2642..2659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2813..3401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2893..2912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3572..4363,4364..4432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4195..4214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4433..4537)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4605..4623
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    4968..5102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5005..5024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5059..5078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5176..5265)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 ctgcaggaat tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa
       61 acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata
      121 ggtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      181 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      241 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      301 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      361 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag gaaagtcgag
      421 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      481 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      541 ccgggaccga tccagcctcc gcggccccga attcgagctc ggtacccggg gatcctctag
      601 tcagctgacg cgtgctagcg cggcctcgac gatggccgct ctagaactag tggatccacc
      661 atggcgtcta tggaacaaaa gctgattagc gaagaggacc tgaacaacgg gaattctcga
      721 gcagccgctg acgtgaagat gagcagctca gaggaggtgt cctggatttc ctggttctgt
      781 gggctccgtg gcaatgaatt cttctgtgaa gtggatgaag actacatcca ggacaaattt
      841 aatcttactg gactcaatga gcaggtccct cactatcgac aagctctaga catgatcttg
      901 gacctggagc ctgatgaaga actggaagac aaccccaacc agagtgacct gattgagcag
      961 gcagccgaga tgctttatgg attgatccac gcccgctaca tccttaccaa ccgtggcatc
     1021 gcccagatgt tggaaaagta ccagcaagga gactttggtt actgtcctcg tgtgtactgt
     1081 gagaaccagc caatgcttcc cattggcctt tcagacatcc caggtgaagc catggtgaag
     1141 ctctactgcc ccaagtgcat ggatgtgtac acacccaagt catcaagaca ccatcacacg
     1201 gatggcgcct acttcggcac tggtttccct cacatgctct tcatggtgca tcccgagtac
     1261 cggcccaaga gacctgccaa ccagtttgtg cccaggctct acggtttcaa gatccatccg
     1321 atggcctacc actgcagctc caagccgcca gcaacttcaa gagcccagtc aagacgattc
     1381 gctgattccc tcgagggggg gccatctcta gactgagaac ttcagggtga gtttggggac
     1441 ccttgattgt tctttctttt tcgctattga aaaattcatg ttatatggag ggggcaaagt
     1501 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
     1561 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
     1621 attttctgta acttttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
     1681 ctttcgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
     1741 agggtaatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
     1801 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
     1861 caactacatc ctggtaatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
     1921 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
     1981 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
     2041 tggcaaagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     2101 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     2161 catcatgaag ccccttgagc atctgacttc tgggtaataa aggaaattta ttttcattgc
     2221 aatagtgtgt gggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     2281 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct
     2341 gccatgaaca aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     2401 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     2461 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     2521 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatga actcgactgc
     2581 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3001 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4561 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4621 ggccctttcg tcttcactcg acgctggtcg agctgatact tcccgtccgc caggggacat
     4681 gccggcgatg ctgaaggtcg cgcgcattcc cgatgaagag gccggttacc gcctgttgac
     4741 ctggtgggac gggcagggcg ccgcccgagt cttgcctcgg cggcgggcgc tctgctcatg
     4801 gagcgcgcgt ccggggacct tgcacagata gcgtggtccg gccagacgac gaggcttgca
     4861 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4921 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5101 tcctctagaa ctaggctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
     5161 agaattcggc ttttaagcgt aatctggaac gtcatatgga taggatcctg catagtccgg
     5221 gacgtcatag ggatagcccg catagtcagg aacatcgtat gggtactgct gagcgccagc
     5281 ggcagctgga acaggcatcc caagggggtt ggcagcagca atcactggtg agcctgccag
     5341 aggtccaagg ggtgaagggg ttggcactga agaaatccct gacatcatat tggcgctgct
     5401 gacgggcgta ctgccccctg gcatgctaga tgaacccatt cgagcctggt ccttcacaac
     5461 agtgtagaaa taggggtgct ccattgcctc tcttgcagta agccgtgact ggtggtcata
     5521 tcgcagcagt ttgtccagga aatccaaggc ctcagggctg acaaggtgct gattttcact
     5581 gtggacaaag cgttcccatc gctttcgaga gtgtctgccc aagatatcat tgaaacgtgg
     5641 atctaattca atgttgtatt tgtcaatata gtcatataaa tcttctgtcc ccagaacctt
     5701 ggctatcctc accaactgat cataattgtc atgtccatgg aaaaatggct ccttccgaaa
     5761 gatcatactt gccagcatac aacccaaact ccacatatcc aaactataat cgtacatctg
     5821 atagtctaca agtagctcag gacctttgaa gtatcgggaa gcaactcgga cattatattc
     5881 ttggccagga tgataaaact cagccaaacc ccagtctatt agtcgtagct ttctgtgctc
     5941 atgatcaatc atgacattat ggggcttgac atctctgtgc ataattccca tgctgtgaca
     6001 ataatccagg gccttcagaa tctcatacat gtaaaatcga atatcatagt ctgttaacgt
     6061 ctggtacaat tgcttgaagt ctgtgttgtt tacgtgttca aaaaccaagg cgggggttcg
     6121 tgacacaggg tcttttacaa tgtctgccag tgtgatgatg ttgggacctc ctctcaaatt
     6181 ctccaaaatc tttatttcac gcttaatttt cttctttttt actggcttga gaatcataac
     6241 aacaactttt tcattatttg tgatgttgat ggcttcaaat acttcactgt atttacctcg
     6301 gcctaatttt cgaaccagct ggtagtcatc ttgatttccc cattccacca catgtgactc
     6361 gtaatcccag tattctcgag gtctgtgtgt attaacatct gtgtaaactc tggccctgct
     6421 tggcacgggt cccgacatgt cagacaggtt ggcggacaaa gctggacttg atgtttggag
     6481 atctggcagt cactgtgttc agaagcagct gggggtaaga ccttgtttca gacctggatc
     6541 cagtagttct agagcggccg ccacccggtg gagctcggta ccaagcttcg acgcggccg
//
