LOCUS       sequence-383621-        9695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dominant negative MEK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Tuano NK, Beesley J, Manning M, Shi W, Perlaza-Jimenez L, 
            Malaver-Ortega LF, Paynter JM, Black D, Civitarese A, McCue K, 
            Hatzipantelis A, Hillman K, Kaufmann S, Sivakumaran H, Polo JM, 
            Reddel RR, Band V, French JD, Edwards SL, Powell DR, Chenevix-Trench
            G, Rosenbluh J
  TITLE     CRISPR screens identify gene targets at breast cancer risk loci.
  JOURNAL   Genome Biol. 2023 Mar 29;24(1):59. doi: 10.1186/s13059-023-02898-w.
  PUBMED    36991492
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2023-03-29"; volume: "24(1):59. doi"; pages: " 
            10.1186/s13059-023-02898-w"
COMMENT     Sequence Label: pLX311-GFP-MEKDD
FEATURES             Location/Qualifiers
     source          1..9695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      89..544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(176..195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     386..407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        570..674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(675..743,744..1535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(893..912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1706..2294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2195..2214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2448..2465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2582..2603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2618..2623,2624..2641,2642..2648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2656..2672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2661..2683
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2680..2696
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2680..2696
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2715..2735
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2717..2735
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2763..2989
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2990..3170
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3217..3342
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3835..4068
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4253..4297
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4528..4738
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4550..4746
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4597..4732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4765..4767,4768..4770,4771..5484)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4765..4767,4768..4770,4771..5481)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4810..4831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5071..5090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5418..5439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5525..5642
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5780..6958
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6011..6949
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6906..6926
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6978..7002
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8186..8210)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8218..8259
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8310..8898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8363..8383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8781..8792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8970..9203
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9281..9402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9318..9337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9372..9391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9442..9577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9597..9616)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9598..9616)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9626..9643)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9626..9642)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9635..9657)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
       61 cagttgcgca gcctgaatgg cgaatcggac gcgccctgta gcggcgcatt aagcgcggcg
      121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      301 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
      481 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      541 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2521 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2581 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2641 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2701 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     2761 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     2821 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     2881 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     2941 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     3001 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3061 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3121 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     3181 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     3241 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     3301 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     3361 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     3421 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     3481 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     3541 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     3601 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     3661 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     3721 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     3781 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     3841 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     3901 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     3961 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     4021 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     4081 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     4141 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     4201 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     4261 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     4321 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     4381 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     4441 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     4501 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     4561 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4621 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4681 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4741 tgcaaaaagc tttctagagg taccatggtg agcaagggcg aggagctgtt caccggggtg
     4801 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4861 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4921 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4981 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5041 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5101 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5161 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5221 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5281 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5341 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5401 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5461 ggcatggacg agctgtacaa gtagaattct agatcttgag acaaatggca gtattcatcc
     5521 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     5581 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     5641 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     5701 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     5761 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     5821 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     5881 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     5941 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     6001 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     6061 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     6121 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     6181 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     6241 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     6301 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     6361 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     6421 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     6481 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     6541 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     6601 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     6661 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     6721 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     6781 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     6841 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     6901 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     6961 ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg catgcccaag aagaagccga
     7021 cgcccatcca gctgaacccg gcccccgacg gctctgcagt taacgggacc agctctgcgg
     7081 agaccaactt ggaggccttg cagaagaagc tggaggagct agagcttgat gagcagcagc
     7141 gaaagcgcct tgaggccttt cttacccaga agcagaaggt gggagaactg aaggatgacg
     7201 actttgagaa gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc aaggtctccc
     7261 acaagccttc tggcctggtc atggccagaa agctaattca tctggagatc aaacccgcaa
     7321 tccggaacca gatcataagg gagctgcagg ttctgcatga gtgcaactct ccgtacatcg
     7381 tgggcttcta tggtgcgttc tacagcgatg gcgagatcag tatctgcatg gagcacatgg
     7441 atggaggttc tctggatcaa gtcctgaaga aagctggaag aattcctgaa caaattttag
     7501 gaaaagttag cattgctgta ataaaaggcc tgacatatct gagggagaag cacaagatca
     7561 tgcacagaga tgtcaagccc tccaacatcc tagtcaactc ccgtggggag atcaagctct
     7621 gtgactttgg ggtcagcggg cagctcatcg acgacatggc caacgacttc gtgggcacaa
     7681 ggtcctacat gtcgccagaa agactccagg ggactcatta ctctgtgcag tcagacatct
     7741 ggagcatggg actgtctctg gtagagatgg cggttgggag gtatcccatc cctcctccag
     7801 atgccaagga gctggagctg atgtttgggt gccaggtgga aggagatgcg gctgagaccc
     7861 cacccaggcc aaggaccccc gggaggcccc ttagctcata cggaatggac agccgacctc
     7921 ccatggcaat ttttgagttg ttggattaca tagtcaacga gcctcctcca aaactgccca
     7981 gtggagtgtt cagtctggaa tttcaagatt ttgtgaataa atgcttaata aaaaaccccg
     8041 cagagagagc agatttgaag caactcatgg ttcatgcttt tatcaagaga tctgatgctg
     8101 aggaagtgga ttttgcaggt tggctctgct ccaccatcgg ccttaaccag cccagcacac
     8161 caacccatgc tgctggcgtc tagaccccca actttcttgt acaaagtggt tgatatcggt
     8221 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtaccggt
     8281 taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     8341 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8401 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8461 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8521 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8581 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8641 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8701 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8761 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8821 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8881 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     8941 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9001 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9181 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     9241 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9301 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9361 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     9421 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9541 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     9601 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9661 accctggcgt tacccaactt aatcgccttg cagca
//
