LOCUS       sequence-383848-        5933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_15a.10
FEATURES             Location/Qualifiers
     source          1..5933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        235..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1191..1230
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(1308..2159)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2146..2985)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3496..4467)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4574..4968
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5006..5092)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5164..5198
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             5207..5218
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc
      361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga
      421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg
      481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag
      541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc
      601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga
      661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg
      721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata
      781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc
      841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc
      901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg
      961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata
     1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta
     1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta
     1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta
     1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg
     1261 tctcgtacaa ttctgaggtc cacacagata gtgtccgtag gccggcctca gcctgccgcc
     1321 ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca gcccagcgcg
     1381 accagctccg gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt cgaaccactg
     1441 gcctctgacg gccagacata gccgcacaag gtatctatgg aagccttgcc ggttttgccg
     1501 gggtcgatcc agccacacag ccgctggtgc agcaggcggg cggtttcgct gtccagcgcc
     1561 cgcacctcgt ccatgctgat gcgcacatgc tggccgccac ccatgacggc ctgcgcgatc
     1621 aaggggttca gggccacgta caggcgcccg tccgcctcgt cgctggcgta ctccgacagc
     1681 agccgaaacc cctgccgctt gcggccattc tgggcgatga tggatacctt ccaaaggcgc
     1741 tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat cgacctctgc cccgatttcc
     1801 tttgccagcg cccgatagct acctttgacc accatggcat cagcggtgac ggcctcccac
     1861 ttgggttcca ggaacagccg gagctgccgt ccgccttcgg tcttgggttc cgggccaagc
     1921 actaggccat taggcccagc catggccacc agcccttgca ggatgcgcag atcatcagcg
     1981 cccagcggct ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc atacgtcacg
     2041 tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc gggggccaga
     2101 cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg cttcaccacg
     2161 gggcaccccc ttgctcttgc gctgcctctc cagcacggcg ggcttgagca ccccgccgtc
     2221 atgccgcctg aaccaccgat cagcgaacgg tgcgccatag ttggccttgc tcacaccgaa
     2281 gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct cggcgctggt
     2341 catgctcgac aggtaggact gccagcggat gttatcgacc agtaccgagc tgccccggct
     2401 ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat ggtgcaggaa
     2461 cacgatagag cacccggtat cggcggcgat ggcctccatg cgaccgatga cctgggccat
     2521 ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca gcaccatcag
     2581 gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca tgatgttggg
     2641 caggctgccg atcagcggct ggatcagcag gccgtcagcc acggcttgcc gttcctcggc
     2701 gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg ggtcttcggc
     2761 gggcaggtag atcaccgggc cggtgggcag ttcgcccacc tccagcagat ccggcccgcc
     2821 tgcaatctgt gcggccagtt gcagggccag catggattta ccggcaccac cgggcgacac
     2881 cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg gtggcggcgc
     2941 tgctgcgaac gcctccagaa tattgatagg cttatgggta gccattgatt gcctcctttg
     3001 caggcagttg gtggttaggc gctggcgggg tcactacccc cgccctgcgc cgctctgagt
     3061 tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa cttgcgctga
     3121 cgcatccctt tggccttcat gcgctcggca tatcgcgctt ggcgtacagc gtcagggctg
     3181 gccagcaggt cgccggtctg cttgtccttt tggtctttca tatcagtcac cgagaaactt
     3241 gccggggccg aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt caaggttaag
     3301 gctggccata tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg tataaccaaa
     3361 gccaccgggc aaccaatagc ccttgtcact tttgatcagg tagaccgacc ctgaagcgct
     3421 tttttcgtat tccataaaac ccccttctgt gcgtgagtac tcatagtata acaggcgtga
     3481 gtaccaacgc aagcactaca tgctgaaatc tggcccgccc ctgtccatgc ctcgctggcg
     3541 gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg acgctgggca gacccatgac
     3601 cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct ctgccagcgc
     3661 tgggctggcc tcggccatgg ccttgccgat ttcctcggca ctgcggcccc ggctggccag
     3721 cttctgcgcg gcgataaagt cgcacttgct gaggtcatca ccgaagcgct tgaccagccc
     3781 ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa gctgccgctc
     3841 gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg cctgctcgat
     3901 ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc ccttggattc
     3961 acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct tgcggttggt
     4021 gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg cgtcgtactc
     4081 gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt cggccacctt
     4141 gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct cccggccctc
     4201 ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca gaccatgccg
     4261 ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc gcttgagcca
     4321 tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct ggccggtggt
     4381 ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct gtcggcctat
     4441 ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gattcccaca cattatacga
     4501 gccggaagca taaagtgtag ctagatccga aggatgagcc gggctgaatg atcgaccgag
     4561 acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa
     4621 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt
     4681 gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag
     4741 accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc
     4801 gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt ttccaacacc
     4861 ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt attgcagggg
     4921 ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg acagttaggg
     4981 cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac
     5041 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgccttt
     5101 aattaaggct cgggagacct atcggtaata acagtccaat ctggtgtaac ttcggaatcg
     5161 tccttgacag ctagctcagt cctaggtata atgctagcta ctagtgaaag aggagaaata
     5221 ctagtatggt tagcaaaggc gaggcggtta tcaaggagtt tatgcgtttt aaggttcaca
     5281 tggagggtag catgaatggt cacgagttcg agatcgaggg tgaaggcgag ggtcgtccgt
     5341 acgaaggcac ccagaccgcg aagctgaaag tgaccaaggg tggcccgctg ccgttcagct
     5401 gggacatcct gagcccgcag ttcatgtatg gcagccgtgc gtttaccaaa cacccggcgg
     5461 acattccgga ttactataag caaagcttcc cggaaggttt taaatgggag cgtgttatga
     5521 acttcgaaga tggtggcgcg gtgaccgtta cccaggacac cagcctggag gatggcaccc
     5581 tgatttacaa ggtgaaactg cgtggcacca actttccgcc ggatggtccg gttatgcaga
     5641 agaaaacgat gggttgggaa gcgagcaccg agcgtctgta tccggaagat ggcgtgctga
     5701 agggtgatat caaaatggcg ctgcgtctga aggacggtgg ccgttacctg gcggatttta
     5761 agaccaccta taaagcgaag aaaccggtgc aaatgccggg tgcgtacaac gttgaccgta
     5821 aactggatat taccagccac aacgaggatt ataccgtggt tgagcaatat gagcgtagcg
     5881 agggtcgcca cagcaccggc ggcatggacg aactgtataa gggatcctaa taa
//
