LOCUS       sequence-383887-        8247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 NSP2_T85I in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 NSP2_T85I
FEATURES             Location/Qualifiers
     source          1..8247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..43)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(197..785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(277..296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(956..1747,1748..1816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1579..1598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1817..1921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1996..2015)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2187..2566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2567..2770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2741
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2772..2790
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2821..2845
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2988..3012
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4936..4960)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5007..5030
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5067..5090
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5121..5147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5121..5147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5148..5495,5496..5498,5499..6107)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6151..6168)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6157..6381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6514..6533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6665..6712
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6720..7742
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6720..7742
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7872..7993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7909..7928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7963..7982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8042..8058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8042..8058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8055..8077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8066..8082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8090..8096,8097..8114,8115..8120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8135..8156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
       61 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1921 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1981 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     2041 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     2101 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     2161 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     2221 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2281 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2341 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2401 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2461 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2521 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2581 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2641 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2701 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2761 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
     2821 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2881 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttggtac
     2941 cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     3001 aaaaagcagg ctccaccatg gcctacacgc gatatgttga caacaacttt tgtggacctg
     3061 acggctaccc tcttgaatgt attaaggatc ttctggctcg cgctggaaaa gccagttgta
     3121 cactgtctga gcagctcgac tttatagata caaagcgggg ggtctattgt tgccgagagc
     3181 atgaacatga gatcgcatgg tatacggagc gctctgaaaa aagctatgaa ttgcagacac
     3241 cctttgaaat taagttggcg aaaaagttcg atatattcaa tggtgagtgc cccaactttg
     3301 tctttcccct taacagtatc attaaaacaa tccagccccg ggtggaaaag aagaaattgg
     3361 atggattcat gggcaggatc agaagtgttt acccagtggc atcccctaat gaatgcaatc
     3421 agatgtgcct gtccacgctg atgaaatgcg accactgcgg tgaaacgtct tggcaaacgg
     3481 gggactttgt aaaagcaacg tgcgagtttt gcggaactga gaacttgaca aaggagggag
     3541 cgactacatg tggatacctg cctcagaacg cggtagtgaa aatatactgt cctgcctgtc
     3601 acaattctga agttgggcct gaacatagct tggccgaata ccataacgaa agtggattga
     3661 agacaattct tcgcaaagga ggccggacta tcgcgttcgg gggctgcgtg tttagctatg
     3721 taggatgtca taataaatgc gcatattggg ttccacgagc tagcgcaaac ataggctgca
     3781 accacacagg cgtagttggt gagggatcag aaggtcttaa tgataacttg ctcgaaatat
     3841 tgcaaaagga aaaagtcaac ataaatattg taggagattt taaattgaat gaagagattg
     3901 cgataatcct tgcgtcattt tccgcaagta cgagtgcgtt cgttgagact gtaaaggggc
     3961 tcgactataa agcgtttaag caaatagtag agagctgcgg aaactttaaa gttacgaagg
     4021 gaaaagccaa aaaaggtgca tggaacattg gcgagcagaa gagtatactg tcccccctgt
     4081 acgccttcgc gtcagaagca gctcgagtag ttagaagtat attctcacga acgctcgaga
     4141 cggcgcagaa tagtgtgcgc gtgctccaga aggctgctat tacgatcctc gacggcatat
     4201 cccagtattc tcttcgactg attgatgcta tgatgttcac ctccgacttg gcgacgaaca
     4261 atctcgtagt tatggcttat atcaccggcg gcgttgtaca attgacgtct cagtggctga
     4321 cgaacatctt cggaaccgtg tacgagaagc tcaaaccagt tcttgattgg cttgaagaaa
     4381 agtttaagga aggcgtcgag tttctccggg atgggtggga aattgttaag tttatctcca
     4441 cctgcgcgtg tgaaattgtg ggagggcaaa ttgtcacctg cgccaaggaa attaaagagt
     4501 cagttcagac gttttttaaa cttgtgaaca aattccttgc tctctgtgcg gatagtataa
     4561 tcatcggtgg ggcgaagttg aaagcactca atcttggtga aactttcgtg acccacagca
     4621 aaggcctgta taggaagtgt gttaaaagca gggaggagac cggtcttctt atgccgctga
     4681 aggcgcccaa agagataatt tttcttgagg gggaaactct tccgaccgaa gtgctcacgg
     4741 aagaagtagt tctgaaaaca ggggaccttc aaccactgga gcaaccgacc tctgaggcag
     4801 ttgaagcgcc gctcgtcgga actcctgtat gtattaacgg cctcatgctg ttggaaatta
     4861 aggatacgga gaagtactgt gcactggccc ccaacatgat ggtcacgaac aacaccttta
     4921 cccttaaggg cggcgaccca gctttcttgt acaaagtggt tgatatcaag gccgccgaca
     4981 tcaccagcct gtacaagaaa gtggggtgga gccatcccca gttcgagaag ggcggaggat
     5041 ctggcggcgg aagcggcgga ggaagctggt cccaccctca gtttgagaaa ggcaccgagc
     5101 tgggcagcac catggccagc tacccctacg acgtgcccga ctacgccaag gacaacaccg
     5161 tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt ccactctggc gagcagctgg
     5221 gcgagacact gggcatgagc agagccgcca tcaacaagca catccagacc ctgcgggact
     5281 ggggcgtgga cgtgttcaca gtgcccggca agggctacag cctgcccgag cccatccagc
     5341 tgctgaacgc caagcagatc ctgggccagc tggatggcgg atctgtggcc gtgctgcccg
     5401 tgatcgacag caccaaccag tatctgctcg accggatcgg cgagctgaag tccggcgacg
     5461 cctgtatcgc cgagtaccag caggccggca gaggcggcag gggcagaaag tggttcagcc
     5521 ccttcggcgc caacctgtac ctgagcatgt tctggagact ggaacagggc ccagccgccg
     5581 ctattggcct gagcctggtc atcggcatcg tgatggccga ggtgctgcgg aagctgggcg
     5641 ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg aagctggccg
     5701 gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg
     5761 gcatcaacat ggccatgcgg cgggtggaag agagcgtggt caaccagggc tggatcaccc
     5821 tgcaggaagc cggcatcaac ctggaccgga acaccctggc cgccatgctg atcagagagc
     5881 tgagagccgc cctggaactg ttcgagcagg aaggcctggc cccctacctg agcagatggg
     5941 agaagctgga caacttcatc aaccggcccg tgaaactgat catcggcgac aaagagatct
     6001 tcggcatcag ccggggcatc gacaagcagg gggccctgct cctggaacag gacggcatca
     6061 tcaagccctg gatgggcggc gagatcagcc tgcggagcgc cgagaagtga tgactcgagt
     6121 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6181 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6241 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6301 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6361 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6421 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6481 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6541 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6601 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6661 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     6721 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     6781 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     6841 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     6901 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     6961 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     7021 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     7081 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     7141 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     7201 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     7261 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     7321 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     7381 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     7441 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     7501 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     7561 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     7621 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     7681 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     7741 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7801 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7861 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7921 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7981 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8041 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8101 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8161 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8221 tcggccaacg cgcggggaga ggcggtt
//
