LOCUS       sequence-383900-        6052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-tagged CNTF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ribeiro M, Ayupe AC, Beckedorff FC, Levay K, Rodriguez S, Tsoulfas 
            P, Lee JK, Nascimento-Dos-Santos G, Park KK
  TITLE     Retinal ganglion cell expression of cytokine enhances occupancy of 
            NG2 cell-derived astrocytes at the nerve injury site: Implication 
            for axon regeneration.
  JOURNAL   Exp Neurol. 2022 Sep;355:114147. doi: 
            10.1016/j.expneurol.2022.114147. Epub 2022 Jun 20.
  PUBMED    35738417
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Neurol."; date: "2022-09"; volume: "355:114147. doi"; pages: " 
            10.1016/j.expneurol.2022.114147. Epub 2022 Jun 20"
COMMENT     Sequence Label: pAAV-CNTF-HA
FEATURES             Location/Qualifiers
     source          1..6052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(96..115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      909..1497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1398..1417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1559..1688
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        1710..2109
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2679..2698
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     ncRNA           3182..3208
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           3272..3313
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     3618..3637
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4321..4347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4357..4833
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4556..4575)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4873..5013
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4873..5002
                     /label=AAV2 ITR
     rep_origin      5088..5543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5175..5194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5385..5406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5560..5579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5679..5701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5739..5757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5825..5929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5930..5998,5999..6052,1..738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc
     1561 tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg cgacctttgg
     1621 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     1681 gggttcctgc ggccgcacgc gtttaaactg gcctccgcgc cgggttttgg cgcctcccgc
     1741 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     1801 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     1861 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     1921 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     1981 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     2041 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     2101 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
     2161 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
     2221 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
     2281 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     2341 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     2401 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     2461 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     2521 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     2581 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     2641 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     2701 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     2761 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     2821 gtttttggct tttttgttag acgaagctaa ggcgcgcctg agaacttcag ggtgagtttg
     2881 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     2941 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tgcatggacc
     3001 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     3061 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
     3121 taaattcact tttgtttatt tgtcagattg taagatccca tcgattcgaa ttcaaagtcg
     3181 actggaggct tgctgaaggc tgtatgctgt ttatgaggat ctctctgatt tttttggcct
     3241 ctgactgaaa atcaagagtc ctcataaaca ggacacaagg cctgttacta gcactcacat
     3301 ggaacaaatg gccaccgtgg gaggatgaca actcgagcct ctagacaatc agggtatatt
     3361 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     3421 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat
     3481 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     3541 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt
     3601 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcaag cttaaggatc
     3661 caaggtacca tgtccatgtt gttctacact ctgatcacag cttttctgat cggcatacag
     3721 gcggctttca cagagcattc accgctgacc cctcaccgtc gggacctctg tagccgctct
     3781 atctggctag caaggaagat tcgttcagac ctgactgctc ttacggaatc ctatgtgaag
     3841 catcagggcc tgaacaagaa catcaacctg gactctgcgg atgggatgcc agtggcaagc
     3901 actgatcagt ggagtgagct gaccgaggca gagcgactcc aagagaacct tcaagcttat
     3961 cgtaccttcc atgttttgtt ggccaggctc ttagaagacc agcaggtgca ttttacccca
     4021 accgaaggtg acttccatca agctatacat acccttcttc tccaagtcgc tgcctttgca
     4081 taccagatag aggagttaat gatactcctg gaatacaaga tcccccgcaa tgaggctgat
     4141 gggatgccta ttaatgttgg agatggtggt ctctttgaga agaagctgtg gggcctaaag
     4201 gtgctgcagg agctttcaca gtggacagta aggtccatcc atgaccttcg tttcatttct
     4261 tctcatcaga ctgggatccc agcacgtggg agccattata ttgctaacaa caagaaaatg
     4321 tacccatacg atgttccaga ttacgcttag atctacgggt ggcatccctg tgacccctcc
     4381 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     4441 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     4501 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     4561 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     4621 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4681 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4741 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4801 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     4861 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4921 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4981 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     5041 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     5101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5161 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5221 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5281 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     5341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5401 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     5461 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5521 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     5581 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5641 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5701 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5761 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5941 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6001 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac ga
//
