LOCUS       sequence-383973-        9182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AURKB and mCherry in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   McNeal AS, Belote RL, Zeng H, Urquijo M, Barker K, Torres R, Curtin 
            M, Shain AH, Andtbacka RH, Holmen S, Lum DH, McCalmont TH, 
            VanBrocklin MW, Grossman D, Wei ML, Lang UE, Judson-Torres RL
  TITLE     BRAF(V600E) induces reversible mitotic arrest in human melanocytes 
            via microrna-mediated suppression of AURKB.
  JOURNAL   Elife. 2021 Nov 23;10. pii: 70385. doi: 10.7554/eLife.70385.
  PUBMED    34812139
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-23"; volume: "10. pii: 70385. doi"; pages: " 
            10.7554/eLife.70385"
COMMENT     Sequence Label: pLVX-Phi3-AURKB-PGK-mCherry
FEATURES             Location/Qualifiers
     source          1..9182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3575..3586
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3902..4401
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3937..3960)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4334..4353
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4420..4429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4426..5136
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4571..4589)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4839..4858
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5150..5738
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5203..5223)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5621..5632)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5945..6578
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6707..6723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6707..6723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6720..6742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6731..6747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6755..6761,6762..6779,6780..6785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6800..6821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6938..6955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7109..7697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7189..7208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7868..8659,8660..8728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8491..8510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8729..8833)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8881..9015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8918..8937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8972..8991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc tgaagcttag atctcgagaa
     2821 ttcgcggccg ctccggacgc gtatggccca gaaggagaac tcctacccct ggccctacgg
     2881 ccgacagacg gctccatctg gcctgagcac cctgccccag cgagtcctcc ggaaagagcc
     2941 tgtcacccca tctgcacttg tcctcatgag ccgctccaat gtccagccca cagctgcccc
     3001 tggccagaag gtgatggaga atagcagtgg gacacccgac atcttaacgc ggcacttcac
     3061 aattgatgac tttgagattg ggcgtcctct gggcaaaggc aagtttggaa acgtgtactt
     3121 ggctcgggag aagaaaagcc atttcatcgt ggcgctcaag gtcctcttca agtcccagat
     3181 agagaaggag ggcgtggagc atcagctgcg cagagagatc gaaatccagg cccacctgca
     3241 ccatcccaac atcctgcgtc tctacaacta tttttatgac cggaggagga tctacttgat
     3301 tctagagtat gccccccgcg gggagctcta caaggagctg cagaagagct gcacatttga
     3361 cgagcagcga acagccacga tcatggagga gttggcagat gctctaatgt actgccatgg
     3421 gaagaaggtg attcacagag acataaagcc agaaaatctg ctcttagggc tcaagggaga
     3481 gctgaagatt gctgacttcg gctggtctgt gcatgcgccc tccctgagga ggaagacaat
     3541 gtgtggcacc ctggactacc tgcccccaga gatgattgag gggcgcatgc acaatgagaa
     3601 ggtggatctg tggtgcattg gagtgctttg ctatgagctg ctggtgggga acccaccctt
     3661 tgagagtgca tcacacaacg agacctatcg ccgcatcgtc aaggtggacc taaagttccc
     3721 cgcttccgtg cccatgggag cccaggacct catctccaaa ctgctcaggc ataacccctc
     3781 ggaacggctg cccctggccc aggtctcagc ccacccttgg gtccgggcca actctcggag
     3841 ggtgctgcct ccctctgccc ttcaatctgt cgcctgatta attaatctag ataattctac
     3901 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     3961 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4021 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     4081 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     4141 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     4201 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     4261 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     4321 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     4381 tcctcatctc cgggcctttc gacctgcctc gaggcccggg ccaccatggt gagcaagggc
     4441 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     4501 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     4561 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     4621 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     4681 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     4741 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     4801 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     4861 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     4921 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     4981 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     5041 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     5101 cactccaccg gcggcatgga cgagctgtac aagtaaccgg gtctggaaca atcaacctct
     5161 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5221 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5281 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5341 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5401 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5461 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5521 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     5581 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     5641 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     5701 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     5761 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5821 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5881 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     5941 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     6001 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     6061 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     6121 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6181 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     6241 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     6301 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6361 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6421 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6481 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6541 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6601 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     6661 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9001 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9061 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9121 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9181 gt
//
