LOCUS       sequence-384032-        4907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage ST32.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_ST32
FEATURES             Location/Qualifiers
     source          1..4907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     231..253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(409..1287)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1314..1598
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1521..1540
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2969..2996
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3015..3106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3107..3175,3176..3967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3325..3344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4138..4726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4627..4646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg
      301 cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
      361 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
      421 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
      481 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
      541 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
      601 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
      661 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
      721 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
      781 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
      841 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
      901 atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca
      961 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
     1021 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
     1081 acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
     1141 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
     1201 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
     1261 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac
     1321 caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct
     1381 aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat
     1441 gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt
     1501 gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac
     1561 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctaacagg
     1621 aggaattaac catgacaagt aaaaaaaaga acaatctatc agtgaaggtg gcggacggcc
     1681 tggaaaatct ggtgaccggc ctgggtggtc aggctgataa aagcaccttt aaccagtgga
     1741 ccttcagcaa caaaaacgcg aattaccgcg agctgatgaa ccgtttccgc gaagattggg
     1801 ttgcgcaaaa agtttgtacc gtcatcccac aggatatgac cagaaagtgg cgtcatattg
     1861 actccgagga aggccgtaag gcggataaga aattgcgcat ccgcaagttg ttccgtgagg
     1921 cgtatcagtg ggcacgtctg tatggtacaa gctttgtttt gttggacctg aagggcactg
     1981 gcaaattaga caccccgttg aggctggaca acttgaagac cgggtgcatt aaaagtatgc
     2041 gtgttattga tcgtagccgt ttgttcgcag cgggtacgat ggttttggac ccgctgtctc
     2101 cgcactacgg tcttccggag tactacacgc tggcaggcta cccgggttat atccactaca
     2161 cccgtttcct gcgcttcgaa ggtactgagc tgccgctgtt cgagttccag cgtaatatgt
     2221 ggtatagcga ttccgtgcta attccattac tgaagaccat tgaccagttt tataccacgg
     2281 cagcggctgc tgcgtccctg gcccaagaag caactatcga cgtggttacc gtcgagggcc
     2341 tgcaaagcct gctgacctcg ccggaaggcg aggcggctgt gatgaagcgc ttcaaactga
     2401 tgaaacttgc caagagcatt tataacgttc tgatcctgga caacaccgag aagtatgata
     2461 ccaaatcgat cgccctgtcc ggtgttaaag atttgatctg ggaatacctg aagatcgtag
     2521 cggctgcggt gggtattccg gcaacccgtt tcctgtctgc gagcccggat ggtatgaatg
     2581 cgaccggcga gtcagatctg gtcaattaca tcgacctctt gaccggtttg caaacctctg
     2641 tttttgaccc gcgtctcgac gttgttgata aaatcgtaca ggcacatttt ggcatcgaag
     2701 agtacaatta cgaatggctg gatatttttc cggaaagcaa cgtggagaaa gccaaacgtg
     2761 caaaggacct cgcttttgcc gtggacctgc tggtcaacaa cggcacgatc accaccgaag
     2821 tggcgaacga agttatgttt cacagcggtg tgttcggtac ctgcgacctg ggcaagccga
     2881 acccgaaccc gcctaatatt caaaaaggta atgcgagcga ggcgaaaccg aaaccgggta
     2941 gcgcgtaatg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     3001 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     3361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4261 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4861 cagccgaacg accgagcgca gcgagtcagt gagcgrggaa gcggaag
//
