LOCUS       sequence-384063-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_D1118H in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_D1118H
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6835..6859)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6906..6929
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6966..6989
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7020..7046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7020..7046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7047..7394,7395..7397,7398..8006)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8050..8067)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8056..8280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8413..8432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8564..8611
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8619..9641
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8619..9641
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9771..9892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9808..9827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9862..9881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9941..9957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9941..9957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9954..9976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9965..9981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9989..9995,9996..10013,10014..10019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10034..10055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     3001 acaaaaaagt tggcatgttc gtgttcctgg tgctgctgcc actggtgagc tcccagtgcg
     3061 tgaacctgac caccagaacc cagctgccac ctgcctacac caactccttc accaggggcg
     3121 tgtactaccc agacaaggtg ttcagatcca gcgtgctgca ctctacccag gacctgttcc
     3181 tgccattctt ctctaacgtg acctggttcc acgccatcca cgtgtctgga accaacggca
     3241 ccaagagatt cgacaaccca gtgctgccat tcaacgacgg cgtgtacttc gcttctaccg
     3301 agaagtctaa catcatcagg ggttggatct tcggaaccac cctggactct aagacccagt
     3361 ctctgctgat cgtgaacaac gctaccaacg tggtgatcaa ggtgtgcgag ttccagttct
     3421 gcaacgaccc attcctggga gtgtactacc acaagaacaa caagtcttgg atggaaagcg
     3481 agttcagggt gtacagctct gctaacaact gcaccttcga gtacgtgtct cagcctttcc
     3541 tgatggacct ggaaggaaag cagggaaact tcaagaacct gagagagttc gtgttcaaga
     3601 acatcgacgg ttacttcaag atatacagca agcacacccc aatcaacctg gtgagggacc
     3661 tgccacaggg tttctctgct ctggagcccc tggtggacct gccaatcggc atcaacatca
     3721 ccaggttcca gaccctgctg gctctgcaca ggtcttacct gaccccaggc gacagcagct
     3781 ctggatggac cgccggcgcc gctgcttact acgtgggata cctgcagcca aggaccttcc
     3841 tgctgaagta caacgaaaac ggcaccatca ccgacgccgt ggactgcgct ctggacccac
     3901 tgtctgagac caagtgcacc ctgaagtctt tcaccgtgga aaagggaatc taccagacca
     3961 gcaacttccg cgtgcagccc accgagtcta tcgtgagatt cccaaacatc accaacctgt
     4021 gcccattcgg agaagtgttc aacgctacca ggttcgccag cgtgtacgct tggaacagga
     4081 agagaatcag caactgcgtg gccgactact ctgtgctgta caactctgcc tccttctcca
     4141 ccttcaagtg ctacggcgtg tccccaacca agctgaacga cctgtgcttc accaacgtgt
     4201 acgctgactc cttcgtgatc agaggagacg aagtgagaca gatcgctccc ggtcagaccg
     4261 gaaagatcgc cgactacaac tacaagctgc cagacgactt caccggttgc gtgatcgctt
     4321 ggaactctaa caacctggac tctaaggtgg gaggcaacta caactacctg tacaggctgt
     4381 tccgcaagag caacctgaag cctttcgaaa gggacatcag caccgaaatc taccaggctg
     4441 gatctacccc atgcaacgga gtggaaggat tcaactgcta cttccctctg cagtcttacg
     4501 gattccagcc aaccaacgga gtgggatacc agccatacag agtggtggtg ctgtctttcg
     4561 agctgctgca cgctcctgct accgtgtgcg gaccaaagaa gtctaccaac ctggtgaaga
     4621 acaagtgcgt gaacttcaac ttcaacggac tgaccggaac cggagtgctg accgaatcca
     4681 acaagaagtt cctgcctttc cagcagttcg gtagagacat cgccgacacc accgacgctg
     4741 tgagagaccc acagaccctg gaaatcctgg acatcacccc ctgctctttc ggaggagtga
     4801 gcgtgatcac cccaggaacc aacacctcca accaggtggc cgtgctgtac caggacgtga
     4861 actgcaccga agtgccagtg gccatccacg ctgaccagct gaccccaacc tggagagtgt
     4921 acagcaccgg atctaacgtg ttccagaccc gtgccggctg cctgatcgga gccgagcacg
     4981 tgaacaactc ctacgaatgc gacatcccaa tcggagctgg catctgcgct tcttaccaga
     5041 cccagaccaa cagcccaaga agggccagat ccgtggcctc tcagagcatc atcgcctaca
     5101 ccatgagcct gggcgctgaa aacagcgtgg cttacagcaa caactctatc gccatcccaa
     5161 ccaacttcac catctctgtg accaccgaaa tcctgccagt gagcatgacc aagaccagcg
     5221 tggactgcac catgtacatc tgcggagact ctaccgaatg ctctaacctg ctgctgcagt
     5281 acggcagctt ctgcacccag ctgaaccgcg ctctgaccgg aatcgctgtg gagcaggaca
     5341 agaacaccca ggaagtgttc gctcaggtga agcagatata caagacccca cctatcaagg
     5401 acttcggagg attcaacttc tctcagatcc tgcccgaccc atctaagccc tctaagaggt
     5461 ctttcatcga agacctgctg ttcaacaagg tgaccctggc cgacgccggt ttcatcaagc
     5521 agtacggtga ctgcctggga gacatcgccg ctagagacct gatctgcgcc cagaagttca
     5581 acggtctgac cgtgctgcca ccactgctga ccgacgagat gatcgcccag tacacctctg
     5641 ccctgctggc tggaaccatc acctctggtt ggaccttcgg agctggcgcc gccctgcaga
     5701 tcccattcgc tatgcagatg gcctaccgtt tcaacggtat cggagtgacc cagaacgtgc
     5761 tgtacgaaaa ccagaagctg atcgctaacc agttcaactc tgctatcgga aagatccagg
     5821 actccctgtc ctctaccgct tctgccctgg gaaagctgca ggacgtggtg aaccagaacg
     5881 cccaggccct gaacaccctg gtgaagcagc tgtcttctaa cttcggagcc atctcctctg
     5941 tgctgaacga catcctgtct aggctggaca aggtggaagc cgaggtgcag atcgacagac
     6001 tgatcaccgg aagactgcag tctctgcaga cctacgtgac ccagcagctg atcagggccg
     6061 ctgaaatcag agcttctgcc aacctggctg ccaccaagat gtccgaatgc gtgctgggcc
     6121 agtctaagag ggtggacttc tgcggaaagg gataccacct gatgtccttc ccacagtctg
     6181 ctccacacgg agtggtgttc ctgcacgtga cctacgtgcc cgcccaggaa aagaacttca
     6241 ccaccgcccc agctatctgc cacgacggaa aggctcactt ccccagggag ggagtgttcg
     6301 tgtctaacgg aacccactgg ttcgtgaccc agaggaactt ctacgagcca cagatcatca
     6361 ccacccacaa caccttcgtg tctggaaact gcgacgtggt gatcggaatc gtgaacaaca
     6421 ccgtgtacga cccactgcag ccagaactgg actctttcaa ggaggaactg gacaagtact
     6481 tcaagaacca cacctctcca gacgtggacc tgggagacat ctccggaatc aacgcctctg
     6541 tggtgaacat ccagaaggaa atcgacagac tgaacgaggt ggccaagaac ctgaacgaat
     6601 ctctgatcga cctgcaggaa ctgggaaagt acgagcagta catcaagtgg ccctggtaca
     6661 tctggctggg tttcatcgct ggtctgatcg ctatcgtgat ggtgaccatc atgctgtgct
     6721 gcatgacctc ttgctgctct tgcctgaagg gatgctgctc ttgcggatct tgctgcaagt
     6781 tcgacgagga cgactccgag cctgtgctga agggtgtgaa gctgcactac acctacccaa
     6841 ctttcttgta caaagtggtt gatatcaagg ccgccgacat caccagcctg tacaagaaag
     6901 tggggtggag ccatccccag ttcgagaagg gcggaggatc tggcggcgga agcggcggag
     6961 gaagctggtc ccaccctcag tttgagaaag gcaccgagct gggcagcacc atggccagct
     7021 acccctacga cgtgcccgac tacgccaagg acaacaccgt gcccctgaag ctgatcgccc
     7081 tgctggccaa cggcgagttc cactctggcg agcagctggg cgagacactg ggcatgagca
     7141 gagccgccat caacaagcac atccagaccc tgcgggactg gggcgtggac gtgttcacag
     7201 tgcccggcaa gggctacagc ctgcccgagc ccatccagct gctgaacgcc aagcagatcc
     7261 tgggccagct ggatggcgga tctgtggccg tgctgcccgt gatcgacagc accaaccagt
     7321 atctgctcga ccggatcggc gagctgaagt ccggcgacgc ctgtatcgcc gagtaccagc
     7381 aggccggcag aggcggcagg ggcagaaagt ggttcagccc cttcggcgcc aacctgtacc
     7441 tgagcatgtt ctggagactg gaacagggcc cagccgccgc tattggcctg agcctggtca
     7501 tcggcatcgt gatggccgag gtgctgcgga agctgggcgc cgacaaagtg cgcgtgaagt
     7561 ggcccaacga cctgtatctg caggaccgga agctggccgg catcctggtg gaactgaccg
     7621 gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg catcaacatg gccatgcggc
     7681 gggtggaaga gagcgtggtc aaccagggct ggatcaccct gcaggaagcc ggcatcaacc
     7741 tggaccggaa caccctggcc gccatgctga tcagagagct gagagccgcc ctggaactgt
     7801 tcgagcagga aggcctggcc ccctacctga gcagatggga gaagctggac aacttcatca
     7861 accggcccgt gaaactgatc atcggcgaca aagagatctt cggcatcagc cggggcatcg
     7921 acaagcaggg ggccctgctc ctggaacagg acggcatcat caagccctgg atgggcggcg
     7981 agatcagcct gcggagcgcc gagaagtgat gactcgagtc tagagggccc gtttaaaccc
     8041 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     8101 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     8161 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8221 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8281 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8521 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     8581 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     8641 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     8701 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     8761 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     8821 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     8881 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     8941 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     9001 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     9061 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     9121 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     9181 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     9241 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     9301 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     9361 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     9421 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     9481 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     9541 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     9601 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     9661 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     9721 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     9781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9901 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     9961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10141 gcg
//
