LOCUS       sequence-384064-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_D138Y in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_D138Y
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..36)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(190..778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(270..289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(949..1740,1741..1809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1572..1591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1810..1914)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1989..2008)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2180..2559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2560..2763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2714..2734
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2765..2783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2786..2804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2814..2838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2981..3005
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6825..6849)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6896..6919
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6956..6979
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7010..7036
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7010..7036
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7037..7384,7385..7387,7388..7996)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8040..8057)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8046..8270
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8403..8422)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8554..8601
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8609..9631
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8609..9631
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9761..9882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9798..9817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9852..9871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9931..9947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9931..9947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9944..9966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9955..9971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9979..9985,9986..10003,10004..10009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10024..10045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
       61 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      601 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      841 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      901 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1081 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1261 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1501 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1801 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1861 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1921 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1981 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     2041 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     2101 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     2161 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2221 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2281 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2341 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2401 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2461 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2521 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2581 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2641 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2701 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2761 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
     2821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2881 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
     2941 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagt
     3001 tggcatgttc gtgttcctgg tgctgctgcc actggtgagc tcccagtgcg tgaacctgac
     3061 caccagaacc cagctgccac ctgcctacac caactccttc accaggggcg tgtactaccc
     3121 agacaaggtg ttcagatcca gcgtgctgca ctctacccag gacctgttcc tgccattctt
     3181 ctctaacgtg acctggttcc acgccatcca cgtgtctgga accaacggca ccaagagatt
     3241 cgacaaccca gtgctgccat tcaacgacgg cgtgtacttc gcttctaccg agaagtctaa
     3301 catcatcagg ggttggatct tcggaaccac cctggactct aagacccagt ctctgctgat
     3361 cgtgaacaac gctaccaacg tggtgatcaa ggtgtgcgag ttccagttct gcaactaccc
     3421 attcctggga gtgtactacc acaagaacaa caagtcttgg atggaaagcg agttcagggt
     3481 gtacagctct gctaacaact gcaccttcga gtacgtgtct cagcctttcc tgatggacct
     3541 ggaaggaaag cagggaaact tcaagaacct gagagagttc gtgttcaaga acatcgacgg
     3601 ttacttcaag atatacagca agcacacccc aatcaacctg gtgagggacc tgccacaggg
     3661 tttctctgct ctggagcccc tggtggacct gccaatcggc atcaacatca ccaggttcca
     3721 gaccctgctg gctctgcaca ggtcttacct gaccccaggc gacagcagct ctggatggac
     3781 cgccggcgcc gctgcttact acgtgggata cctgcagcca aggaccttcc tgctgaagta
     3841 caacgaaaac ggcaccatca ccgacgccgt ggactgcgct ctggacccac tgtctgagac
     3901 caagtgcacc ctgaagtctt tcaccgtgga aaagggaatc taccagacca gcaacttccg
     3961 cgtgcagccc accgagtcta tcgtgagatt cccaaacatc accaacctgt gcccattcgg
     4021 agaagtgttc aacgctacca ggttcgccag cgtgtacgct tggaacagga agagaatcag
     4081 caactgcgtg gccgactact ctgtgctgta caactctgcc tccttctcca ccttcaagtg
     4141 ctacggcgtg tccccaacca agctgaacga cctgtgcttc accaacgtgt acgctgactc
     4201 cttcgtgatc agaggagacg aagtgagaca gatcgctccc ggtcagaccg gaaagatcgc
     4261 cgactacaac tacaagctgc cagacgactt caccggttgc gtgatcgctt ggaactctaa
     4321 caacctggac tctaaggtgg gaggcaacta caactacctg tacaggctgt tccgcaagag
     4381 caacctgaag cctttcgaaa gggacatcag caccgaaatc taccaggctg gatctacccc
     4441 atgcaacgga gtggaaggat tcaactgcta cttccctctg cagtcttacg gattccagcc
     4501 aaccaacgga gtgggatacc agccatacag agtggtggtg ctgtctttcg agctgctgca
     4561 cgctcctgct accgtgtgcg gaccaaagaa gtctaccaac ctggtgaaga acaagtgcgt
     4621 gaacttcaac ttcaacggac tgaccggaac cggagtgctg accgaatcca acaagaagtt
     4681 cctgcctttc cagcagttcg gtagagacat cgccgacacc accgacgctg tgagagaccc
     4741 acagaccctg gaaatcctgg acatcacccc ctgctctttc ggaggagtga gcgtgatcac
     4801 cccaggaacc aacacctcca accaggtggc cgtgctgtac caggacgtga actgcaccga
     4861 agtgccagtg gccatccacg ctgaccagct gaccccaacc tggagagtgt acagcaccgg
     4921 atctaacgtg ttccagaccc gtgccggctg cctgatcgga gccgagcacg tgaacaactc
     4981 ctacgaatgc gacatcccaa tcggagctgg catctgcgct tcttaccaga cccagaccaa
     5041 cagcccaaga agggccagat ccgtggcctc tcagagcatc atcgcctaca ccatgagcct
     5101 gggcgctgaa aacagcgtgg cttacagcaa caactctatc gccatcccaa ccaacttcac
     5161 catctctgtg accaccgaaa tcctgccagt gagcatgacc aagaccagcg tggactgcac
     5221 catgtacatc tgcggagact ctaccgaatg ctctaacctg ctgctgcagt acggcagctt
     5281 ctgcacccag ctgaaccgcg ctctgaccgg aatcgctgtg gagcaggaca agaacaccca
     5341 ggaagtgttc gctcaggtga agcagatata caagacccca cctatcaagg acttcggagg
     5401 attcaacttc tctcagatcc tgcccgaccc atctaagccc tctaagaggt ctttcatcga
     5461 agacctgctg ttcaacaagg tgaccctggc cgacgccggt ttcatcaagc agtacggtga
     5521 ctgcctggga gacatcgccg ctagagacct gatctgcgcc cagaagttca acggtctgac
     5581 cgtgctgcca ccactgctga ccgacgagat gatcgcccag tacacctctg ccctgctggc
     5641 tggaaccatc acctctggtt ggaccttcgg agctggcgcc gccctgcaga tcccattcgc
     5701 tatgcagatg gcctaccgtt tcaacggtat cggagtgacc cagaacgtgc tgtacgaaaa
     5761 ccagaagctg atcgctaacc agttcaactc tgctatcgga aagatccagg actccctgtc
     5821 ctctaccgct tctgccctgg gaaagctgca ggacgtggtg aaccagaacg cccaggccct
     5881 gaacaccctg gtgaagcagc tgtcttctaa cttcggagcc atctcctctg tgctgaacga
     5941 catcctgtct aggctggaca aggtggaagc cgaggtgcag atcgacagac tgatcaccgg
     6001 aagactgcag tctctgcaga cctacgtgac ccagcagctg atcagggccg ctgaaatcag
     6061 agcttctgcc aacctggctg ccaccaagat gtccgaatgc gtgctgggcc agtctaagag
     6121 ggtggacttc tgcggaaagg gataccacct gatgtccttc ccacagtctg ctccacacgg
     6181 agtggtgttc ctgcacgtga cctacgtgcc cgcccaggaa aagaacttca ccaccgcccc
     6241 agctatctgc cacgacggaa aggctcactt ccccagggag ggagtgttcg tgtctaacgg
     6301 aacccactgg ttcgtgaccc agaggaactt ctacgagcca cagatcatca ccaccgacaa
     6361 caccttcgtg tctggaaact gcgacgtggt gatcggaatc gtgaacaaca ccgtgtacga
     6421 cccactgcag ccagaactgg actctttcaa ggaggaactg gacaagtact tcaagaacca
     6481 cacctctcca gacgtggacc tgggagacat ctccggaatc aacgcctctg tggtgaacat
     6541 ccagaaggaa atcgacagac tgaacgaggt ggccaagaac ctgaacgaat ctctgatcga
     6601 cctgcaggaa ctgggaaagt acgagcagta catcaagtgg ccctggtaca tctggctggg
     6661 tttcatcgct ggtctgatcg ctatcgtgat ggtgaccatc atgctgtgct gcatgacctc
     6721 ttgctgctct tgcctgaagg gatgctgctc ttgcggatct tgctgcaagt tcgacgagga
     6781 cgactccgag cctgtgctga agggtgtgaa gctgcactac acctacccaa ctttcttgta
     6841 caaagtggtt gatatcaagg ccgccgacat caccagcctg tacaagaaag tggggtggag
     6901 ccatccccag ttcgagaagg gcggaggatc tggcggcgga agcggcggag gaagctggtc
     6961 ccaccctcag tttgagaaag gcaccgagct gggcagcacc atggccagct acccctacga
     7021 cgtgcccgac tacgccaagg acaacaccgt gcccctgaag ctgatcgccc tgctggccaa
     7081 cggcgagttc cactctggcg agcagctggg cgagacactg ggcatgagca gagccgccat
     7141 caacaagcac atccagaccc tgcgggactg gggcgtggac gtgttcacag tgcccggcaa
     7201 gggctacagc ctgcccgagc ccatccagct gctgaacgcc aagcagatcc tgggccagct
     7261 ggatggcgga tctgtggccg tgctgcccgt gatcgacagc accaaccagt atctgctcga
     7321 ccggatcggc gagctgaagt ccggcgacgc ctgtatcgcc gagtaccagc aggccggcag
     7381 aggcggcagg ggcagaaagt ggttcagccc cttcggcgcc aacctgtacc tgagcatgtt
     7441 ctggagactg gaacagggcc cagccgccgc tattggcctg agcctggtca tcggcatcgt
     7501 gatggccgag gtgctgcgga agctgggcgc cgacaaagtg cgcgtgaagt ggcccaacga
     7561 cctgtatctg caggaccgga agctggccgg catcctggtg gaactgaccg gcaaaacagg
     7621 cgacgccgcc cagatcgtga tcggcgctgg catcaacatg gccatgcggc gggtggaaga
     7681 gagcgtggtc aaccagggct ggatcaccct gcaggaagcc ggcatcaacc tggaccggaa
     7741 caccctggcc gccatgctga tcagagagct gagagccgcc ctggaactgt tcgagcagga
     7801 aggcctggcc ccctacctga gcagatggga gaagctggac aacttcatca accggcccgt
     7861 gaaactgatc atcggcgaca aagagatctt cggcatcagc cggggcatcg acaagcaggg
     7921 ggccctgctc ctggaacagg acggcatcat caagccctgg atgggcggcg agatcagcct
     7981 gcggagcgcc gagaagtgat gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     8041 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     8101 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     8161 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     8221 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     8281 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     8341 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8401 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8461 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8521 aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
     8581 tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
     8641 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     8701 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     8761 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     8821 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     8881 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     8941 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     9001 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     9061 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     9121 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     9181 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     9241 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     9301 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     9361 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     9421 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     9481 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     9541 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     9601 ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
     9661 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9721 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9781 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9841 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9901 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9961 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10021 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10081 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10141 tat
//
