LOCUS       sequence-384067-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_D614G in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_D614G
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2371..2574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2576..2594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2625..2649
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2792..2816
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6636..6660)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6707..6730
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6767..6790
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6821..6847
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6821..6847
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6848..7195,7196..7198,7199..7807)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7851..7868)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7857..8081
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8214..8233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8365..8412
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8420..9442
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8420..9442
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9572..9693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9609..9628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9663..9682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9742..9758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9742..9758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9755..9777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9766..9782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9790..9796,9797..9814,9815..9820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9835..9856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9973..9990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct
     2581 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
     2641 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     2701 cgatccagcc tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact
     2761 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatgtt
     2821 cgtgttcctg gtgctgctgc cactggtgag ctcccagtgc gtgaacctga ccaccagaac
     2881 ccagctgcca cctgcctaca ccaactcctt caccaggggc gtgtactacc cagacaaggt
     2941 gttcagatcc agcgtgctgc actctaccca ggacctgttc ctgccattct tctctaacgt
     3001 gacctggttc cacgccatcc acgtgtctgg aaccaacggc accaagagat tcgacaaccc
     3061 agtgctgcca ttcaacgacg gcgtgtactt cgcttctacc gagaagtcta acatcatcag
     3121 gggttggatc ttcggaacca ccctggactc taagacccag tctctgctga tcgtgaacaa
     3181 cgctaccaac gtggtgatca aggtgtgcga gttccagttc tgcaacgacc cattcctggg
     3241 agtgtactac cacaagaaca acaagtcttg gatggaaagc gagttcaggg tgtacagctc
     3301 tgctaacaac tgcaccttcg agtacgtgtc tcagcctttc ctgatggacc tggaaggaaa
     3361 gcagggaaac ttcaagaacc tgagagagtt cgtgttcaag aacatcgacg gttacttcaa
     3421 gatatacagc aagcacaccc caatcaacct ggtgagggac ctgccacagg gtttctctgc
     3481 tctggagccc ctggtggacc tgccaatcgg catcaacatc accaggttcc agaccctgct
     3541 ggctctgcac aggtcttacc tgaccccagg cgacagcagc tctggatgga ccgccggcgc
     3601 cgctgcttac tacgtgggat acctgcagcc aaggaccttc ctgctgaagt acaacgaaaa
     3661 cggcaccatc accgacgccg tggactgcgc tctggaccca ctgtctgaga ccaagtgcac
     3721 cctgaagtct ttcaccgtgg aaaagggaat ctaccagacc agcaacttcc gcgtgcagcc
     3781 caccgagtct atcgtgagat tcccaaacat caccaacctg tgcccattcg gagaagtgtt
     3841 caacgctacc aggttcgcca gcgtgtacgc ttggaacagg aagagaatca gcaactgcgt
     3901 ggccgactac tctgtgctgt acaactctgc ctccttctcc accttcaagt gctacggcgt
     3961 gtccccaacc aagctgaacg acctgtgctt caccaacgtg tacgctgact ccttcgtgat
     4021 cagaggagac gaagtgagac agatcgctcc cggtcagacc ggaaagatcg ccgactacaa
     4081 ctacaagctg ccagacgact tcaccggttg cgtgatcgct tggaactcta acaacctgga
     4141 ctctaaggtg ggaggcaact acaactacct gtacaggctg ttccgcaaga gcaacctgaa
     4201 gcctttcgaa agggacatca gcaccgaaat ctaccaggct ggatctaccc catgcaacgg
     4261 agtggaagga ttcaactgct acttccctct gcagtcttac ggattccagc caaccaacgg
     4321 agtgggatac cagccataca gagtggtggt gctgtctttc gagctgctgc acgctcctgc
     4381 taccgtgtgc ggaccaaaga agtctaccaa cctggtgaag aacaagtgcg tgaacttcaa
     4441 cttcaacgga ctgaccggaa ccggagtgct gaccgaatcc aacaagaagt tcctgccttt
     4501 ccagcagttc ggtagagaca tcgccgacac caccgacgct gtgagagacc cacagaccct
     4561 ggaaatcctg gacatcaccc cctgctcttt cggaggagtg agcgtgatca ccccaggaac
     4621 caacacctcc aaccaggtgg ccgtgctgta ccagggcgtg aactgcaccg aagtgccagt
     4681 ggccatccac gctgaccagc tgaccccaac ctggagagtg tacagcaccg gatctaacgt
     4741 gttccagacc cgtgccggct gcctgatcgg agccgagcac gtgaacaact cctacgaatg
     4801 cgacatccca atcggagctg gcatctgcgc ttcttaccag acccagacca acagcccaag
     4861 aagggccaga tccgtggcct ctcagagcat catcgcctac accatgagcc tgggcgctga
     4921 aaacagcgtg gcttacagca acaactctat cgccatccca accaacttca ccatctctgt
     4981 gaccaccgaa atcctgccag tgagcatgac caagaccagc gtggactgca ccatgtacat
     5041 ctgcggagac tctaccgaat gctctaacct gctgctgcag tacggcagct tctgcaccca
     5101 gctgaaccgc gctctgaccg gaatcgctgt ggagcaggac aagaacaccc aggaagtgtt
     5161 cgctcaggtg aagcagatat acaagacccc acctatcaag gacttcggag gattcaactt
     5221 ctctcagatc ctgcccgacc catctaagcc ctctaagagg tctttcatcg aagacctgct
     5281 gttcaacaag gtgaccctgg ccgacgccgg tttcatcaag cagtacggtg actgcctggg
     5341 agacatcgcc gctagagacc tgatctgcgc ccagaagttc aacggtctga ccgtgctgcc
     5401 accactgctg accgacgaga tgatcgccca gtacacctct gccctgctgg ctggaaccat
     5461 cacctctggt tggaccttcg gagctggcgc cgccctgcag atcccattcg ctatgcagat
     5521 ggcctaccgt ttcaacggta tcggagtgac ccagaacgtg ctgtacgaaa accagaagct
     5581 gatcgctaac cagttcaact ctgctatcgg aaagatccag gactccctgt cctctaccgc
     5641 ttctgccctg ggaaagctgc aggacgtggt gaaccagaac gcccaggccc tgaacaccct
     5701 ggtgaagcag ctgtcttcta acttcggagc catctcctct gtgctgaacg acatcctgtc
     5761 taggctggac aaggtggaag ccgaggtgca gatcgacaga ctgatcaccg gaagactgca
     5821 gtctctgcag acctacgtga cccagcagct gatcagggcc gctgaaatca gagcttctgc
     5881 caacctggct gccaccaaga tgtccgaatg cgtgctgggc cagtctaaga gggtggactt
     5941 ctgcggaaag ggataccacc tgatgtcctt cccacagtct gctccacacg gagtggtgtt
     6001 cctgcacgtg acctacgtgc ccgcccagga aaagaacttc accaccgccc cagctatctg
     6061 ccacgacgga aaggctcact tccccaggga gggagtgttc gtgtctaacg gaacccactg
     6121 gttcgtgacc cagaggaact tctacgagcc acagatcatc accaccgaca acaccttcgt
     6181 gtctggaaac tgcgacgtgg tgatcggaat cgtgaacaac accgtgtacg acccactgca
     6241 gccagaactg gactctttca aggaggaact ggacaagtac ttcaagaacc acacctctcc
     6301 agacgtggac ctgggagaca tctccggaat caacgcctct gtggtgaaca tccagaagga
     6361 aatcgacaga ctgaacgagg tggccaagaa cctgaacgaa tctctgatcg acctgcagga
     6421 actgggaaag tacgagcagt acatcaagtg gccctggtac atctggctgg gtttcatcgc
     6481 tggtctgatc gctatcgtga tggtgaccat catgctgtgc tgcatgacct cttgctgctc
     6541 ttgcctgaag ggatgctgct cttgcggatc ttgctgcaag ttcgacgagg acgactccga
     6601 gcctgtgctg aagggtgtga agctgcacta cacctaccca actttcttgt acaaagtggt
     6661 tgatatcaag gccgccgaca tcaccagcct gtacaagaaa gtggggtgga gccatcccca
     6721 gttcgagaag ggcggaggat ctggcggcgg aagcggcgga ggaagctggt cccaccctca
     6781 gtttgagaaa ggcaccgagc tgggcagcac catggccagc tacccctacg acgtgcccga
     6841 ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt
     6901 ccactctggc gagcagctgg gcgagacact gggcatgagc agagccgcca tcaacaagca
     6961 catccagacc ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca agggctacag
     7021 cctgcccgag cccatccagc tgctgaacgc caagcagatc ctgggccagc tggatggcgg
     7081 atctgtggcc gtgctgcccg tgatcgacag caccaaccag tatctgctcg accggatcgg
     7141 cgagctgaag tccggcgacg cctgtatcgc cgagtaccag caggccggca gaggcggcag
     7201 gggcagaaag tggttcagcc ccttcggcgc caacctgtac ctgagcatgt tctggagact
     7261 ggaacagggc ccagccgccg ctattggcct gagcctggtc atcggcatcg tgatggccga
     7321 ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct
     7381 gcaggaccgg aagctggccg gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc
     7441 ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg cgggtggaag agagcgtggt
     7501 caaccagggc tggatcaccc tgcaggaagc cggcatcaac ctggaccgga acaccctggc
     7561 cgccatgctg atcagagagc tgagagccgc cctggaactg ttcgagcagg aaggcctggc
     7621 cccctacctg agcagatggg agaagctgga caacttcatc aaccggcccg tgaaactgat
     7681 catcggcgac aaagagatct tcggcatcag ccggggcatc gacaagcagg gggccctgct
     7741 cctggaacag gacggcatca tcaagccctg gatgggcggc gagatcagcc tgcggagcgc
     7801 cgagaagtga tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     7861 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7921 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7981 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8041 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     8101 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     8161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8341 ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag
     8401 tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     8461 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     8521 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     8581 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     8641 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     8701 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     8761 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     8821 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     8881 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     8941 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     9001 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     9061 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     9121 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     9181 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     9241 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     9301 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     9361 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     9421 tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta
     9481 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     9541 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     9601 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     9721 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9781 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9841 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9901 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9961 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10021 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10081 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10141 gtt
//
