LOCUS       sequence-384350-       11238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector with mCherry reporter containing 13 
            copies of the p53-binding consensus sequence (constitutive puromycin
            resistance and EGFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, 
            Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick 
            J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, 
            Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos 
            DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, 
            Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, 
            Scholz SW, Priebe T, Ottl M, Dong J, Meijer M, Janssen LJM, Lourenco
            VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, 
            Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish 
            CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud 
            Aristoy C, Verstreken P, Heinrich L, Chen MY, Schule B, Dou D, 
            Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna 
            R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, 
            Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, 
            Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, 
            Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT
  TITLE     A reference human induced pluripotent stem cell line for large-scale
            collaborative studies.
  JOURNAL   Cell Stem Cell. 2022 Dec 1;29(12):1685-1702.e22. doi: 
            10.1016/j.stem.2022.11.004.
  PUBMED    36459969
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2022.11"; journalName: "Cell Stem Cell"; date: 
            "2022-12-1- 1"; volume: "29"; issue: "12"; pages: "1685-1702.e22"
COMMENT     Sequence Label: PB-PG13-mCherry-EF1a-PuroR-EGFP
FEATURES             Location/Qualifiers
     source          1..11238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..67
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    252..285
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2014..2030)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2014..2030)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2084..2794
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2229..2247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2497..2516
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2807..3395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2860..2880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3278..3289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3415..3639
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3818..5000
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4049..4991
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4948..4968
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5002..5026
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5108..5704
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5108..5127)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5604..5624
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5714..5770
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5777..5779,5780..5782,5783..6496)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5777..5779,5780..5782,5783..6493)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5822..5843)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6083..6102)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6430..6451
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6513..6737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7984..8017
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   8292..8326
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8359..8378)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8360..8378)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8388..8405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8388..8404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8397..8419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8546..9001
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8633..8652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8843..8864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9027..9131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9132..9200,9201..9992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9350..9369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10163..10751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10652..10671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10905..10922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11039..11060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11075..11080,11081..11098,11099..11105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11113..11129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11118..11140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11137..11153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11137..11153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11172..11192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11174..11192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg
       61 acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa
      121 gttttattat atttacactt acatactaat aataaattca acaaacaatt tatttatgtt
      181 tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt
      241 taactagcgc tataacttcg tataatgtat gctatacgaa gttatgagct cacggggaca
      301 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
      361 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
      421 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
      481 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag
      541 agagatttag aatgacagaa tcatagaacg gcctgggttg caaaggagca cagtgctcat
      601 ccagatccaa ccccctgcta tgtgcagggt catcaaccag cagcccaggc tgcccagagc
      661 cacatccagc ctggccttga atgcctgcag ggatggggca tccacagcct ccttgggcaa
      721 cctgttcagt gcgtcaccac cctctggggg aaaaactgcc tcctcatatc caacccaaac
      781 ctcccctgtc tcagtgtaaa gccattcccc cttgtcctat caagggggag tttgctgtga
      841 cattgttggt ctggggtgac acatgtttgc caattcagtg catcacggag aggcagatct
      901 tggggataag gaagtgcagg acagcatgga cgtgggacat gcaggtgttg agggctctgg
      961 gacactctcc aagtcacagc gttcagaaca gccttaagga taagaagata ggatagaagg
     1021 acaaagagca agttaaaacc cagcatggag aggagcacaa aaaggccaca gacactgctg
     1081 gtccctgtgt ctgagcctgc atgtttgatg gtgtctggat gcaagcagaa ggggtggaag
     1141 agcttgcctg gagagataca gctgggtcag taggactggg acaggcagct ggagaattgc
     1201 catgtagatg ttcatacaat cgtcaaatca tgaaggctgg aaaagccctc caagatcccc
     1261 aagaccaacc ccaacccacc caccgtgccc actggccatg tccctcagtg ccacatcccc
     1321 acagttcttc atcacctcca gggacggtga cccccccacc tccgtgggca gctgtgccac
     1381 tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc cagcccgacc ctcccctggc
     1441 acaacgtaag gccattatct ctcatccaac tccaggacgg agtcagtgag gatgggggaa
     1501 tattgctcga cttcgaaggt atcgataagc ttgatgccag gcaagtccag gcaggccagg
     1561 caagtccagg caggccaggc aagtccaggc aggccaggca agtccaggca ggccaggcaa
     1621 gtccaggcag gccaggcaag tccaggcagg ccaggcaagt ccaggcaggc caggcaagtc
     1681 caggcaggcc aggcaagtcc aggcaggcca ggcaagtcca ggcagggcca ggcaagtcca
     1741 ggcaggccag gcaagtccag gcaggccagg caagtccagg caggatcgaa ttcgatctgt
     1801 tttttttagt attaagcaga ggccgggggc ccctggcctc cgcttactct ggagaaaaag
     1861 aagagaggca ttccagaggc aacttgtcaa aacaggactg gcgccttgga ggcgctgtgg
     1921 ggccacccaa attgatataa ttaagcccca accgcctctt cccgcctcat ttcagcctca
     1981 ccaccctcga caagcttatc gataccgtcg ggggatccac tagttctaga gcggccgcca
     2041 ccgcggtgga gctcgagctt ggcattccgg tactgttggt aaaatggtga gcaagggcga
     2101 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2161 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2221 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2281 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2341 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     2401 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     2461 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2521 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     2581 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     2641 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     2701 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     2761 ctccaccggc ggcatggacg agctgtacaa gtaggctagc tacgtaaatc aacctctgga
     2821 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2881 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2941 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3001 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     3061 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3181 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     3241 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3301 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3361 gacgagtcgg atctcccttt gggccgcctc cccgcctgcg ctgatcagcc tcgactgtgc
     3421 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3481 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3541 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3601 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     3661 gctgggagta attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg
     3721 ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg
     3781 ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc
     3841 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     3901 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     3961 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     4021 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     4081 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt
     4141 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     4201 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcttgggcgc tggggccgcc
     4261 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     4321 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     4381 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     4441 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     4501 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     4561 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     4621 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     4681 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     4741 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     4801 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     4861 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     4921 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     4981 ttccatttca ggtgtcgtga gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     5041 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccgc
     5101 cgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     5161 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5221 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5281 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5341 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5401 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5461 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5521 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5581 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5641 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5701 tgccggcagc ggcgccacaa acttctctct gctaaagcaa gcaggtgatg ttgaagaaaa
     5761 ccccgggcct gttaacatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5821 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     5881 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5941 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     6001 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6061 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6121 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6181 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     6241 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     6301 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     6361 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     6421 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     6481 cgagctgtac aagtaacgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6541 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6601 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6661 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6721 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tgggagctca cggggacagc
     6781 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     6841 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     6901 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     6961 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctttag gctgaaagag
     7021 agatttagaa tgacagaatc atagaacggc ctgggttgca aaggagcaca gtgctcatcc
     7081 agatccaacc ccctgctatg tgcagggtca tcaaccagca gcccaggctg cccagagcca
     7141 catccagcct ggccttgaat gcctgcaggg atggggcatc cacagcctcc ttgggcaacc
     7201 tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc ctcatatcca acccaaacct
     7261 cccctgtctc agtgtaaagc cattccccct tgtcctatca agggggagtt tgctgtgaca
     7321 ttgttggtct ggggtgacac atgtttgcca attcagtgca tcacggagag gcagatcttg
     7381 gggataagga agtgcaggac agcatggacg tgggacatgc aggtgttgag ggctctggga
     7441 cactctccaa gtcacagcgt tcagaacagc cttaaggata agaagatagg atagaaggac
     7501 aaagagcaag ttaaaaccca gcatggagag gagcacaaaa aggccacaga cactgctggt
     7561 ccctgtgtct gagcctgcat gtttgatggt gtctggatgc aagcagaagg ggtggaagag
     7621 cttgcctgga gagatacagc tgggtcagta ggactgggac aggcagctgg agaattgcca
     7681 tgtagatgtt catacaatcg tcaaatcatg aaggctggaa aagccctcca agatccccaa
     7741 gaccaacccc aacccaccca ccgtgcccac tggccatgtc cctcagtgcc acatccccac
     7801 agttcttcat cacctccagg gacggtgacc cccccacctc cgtgggcagc tgtgccactg
     7861 cagcaccgct ctttggagaa ggtaaatctt gctaaatcca gcccgaccct cccctggcac
     7921 aacgtaaggc cattatctct catccaactc caggacggag tcagtgagga tgggggaata
     7981 ttgataactt cgtataatgt atgctatacg aagttatgat atctataaca agaaaatata
     8041 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     8101 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     8161 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     8221 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     8281 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagaatcg
     8341 atggcgcgcc ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     8401 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8461 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8521 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8581 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8641 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8701 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8761 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8821 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8881 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8941 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9001 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9061 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggattaa ttaagcggcc
    11221 gcatacgcgt atactaga
//
