LOCUS       sequence-384448-        9453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_N501Y in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: HA-C Sars-CoV-2 S_N501Y
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(8..596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(88..107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(767..1558,1559..1627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1390..1409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1628..1732)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1807..1826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1998..2377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2378..2581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2532..2552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2583..2601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2604..2622
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2632..2656
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2799..2823
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6913..6937)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6984..7007
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7044..7067
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7098..7124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7098..7124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7168..7185)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7174..7398
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7531..7550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7682..7729
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7737..8759
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7737..8759
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8889..9010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8926..8945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8980..8999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9059..9075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9059..9075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9072..9094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9083..9099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9107..9113,9114..9131,9132..9137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9152..9173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9290..9307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
       61 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1741 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     1801 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     1861 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     1921 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     1981 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     2041 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2101 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2161 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2221 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2281 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2341 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2401 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2461 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2521 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2581 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
     2641 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     2701 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagcttggta ccgagctcgg
     2761 atccactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagttg
     2821 gcatgttcgt gttcctggtg ctgctgccac tggtgagctc ccagtgcgtg aacctgacca
     2881 ccagaaccca gctgccacct gcctacacca actccttcac caggggcgtg tactacccag
     2941 acaaggtgtt cagatccagc gtgctgcact ctacccagga cctgttcctg ccattcttct
     3001 ctaacgtgac ctggttccac gccatccacg tgtctggaac caacggcacc aagagattcg
     3061 acaacccagt gctgccattc aacgacggcg tgtacttcgc ttctaccgag aagtctaaca
     3121 tcatcagggg ttggatcttc ggaaccaccc tggactctaa gacccagtct ctgctgatcg
     3181 tgaacaacgc taccaacgtg gtgatcaagg tgtgcgagtt ccagttctgc aacgacccat
     3241 tcctgggagt gtactaccac aagaacaaca agtcttggat ggaaagcgag ttcagggtgt
     3301 acagctctgc taacaactgc accttcgagt acgtgtctca gcctttcctg atggacctgg
     3361 aaggaaagca gggaaacttc aagaacctga gagagttcgt gttcaagaac atcgacggtt
     3421 acttcaagat atacagcaag cacaccccaa tcaacctggt gagggacctg ccacagggtt
     3481 tctctgctct ggagcccctg gtggacctgc caatcggcat caacatcacc aggttccaga
     3541 ccctgctggc tctgcacagg tcttacctga ccccaggcga cagcagctct ggatggaccg
     3601 ccggcgccgc tgcttactac gtgggatacc tgcagccaag gaccttcctg ctgaagtaca
     3661 acgaaaacgg caccatcacc gacgccgtgg actgcgctct ggacccactg tctgagacca
     3721 agtgcaccct gaagtctttc accgtggaaa agggaatcta ccagaccagc aacttccgcg
     3781 tgcagcccac cgagtctatc gtgagattcc caaacatcac caacctgtgc ccattcggag
     3841 aagtgttcaa cgctaccagg ttcgccagcg tgtacgcttg gaacaggaag agaatcagca
     3901 actgcgtggc cgactactct gtgctgtaca actctgcctc cttctccacc ttcaagtgct
     3961 acggcgtgtc cccaaccaag ctgaacgacc tgtgcttcac caacgtgtac gctgactcct
     4021 tcgtgatcag aggagacgaa gtgagacaga tcgctcccgg tcagaccgga aagatcgccg
     4081 actacaacta caagctgcca gacgacttca ccggttgcgt gatcgcttgg aactctaaca
     4141 acctggactc taaggtggga ggcaactaca actacctgta caggctgttc cgcaagagca
     4201 acctgaagcc tttcgaaagg gacatcagca ccgaaatcta ccaggctgga tctaccccat
     4261 gcaacggagt ggaaggattc aactgctact tccctctgca gtcttacgga ttccagccaa
     4321 cctacggagt gggataccag ccatacagag tggtggtgct gtgattccag ccaaccaacg
     4381 gagtgggata ccagccatac agagtggtgg tgctgtgatt ccagccaacc aacggagtgg
     4441 gataccagcc atacagagtg gtggtgctgt gattccagcc aaccwacgga gtgggatacc
     4501 agccatacag agtggtggtg ctgtgattcc agccaaccta cggagtggga taccagccat
     4561 acagagtggt ggtgctgtga ttccagccaa cctacggagt gggataccag ccatacagag
     4621 tggtggtgct gtctttcgag ctgctgcacg ctcctgctac cgtgtgcgga ccaaagaagt
     4681 ctaccaacct ggtgaagaac aagtgcgtga acttcaactt caacggactg accggaaccg
     4741 gagtgctgac cgaatccaac aagaagttcc tgcctttcca gcagttcggt agagacatcg
     4801 ccgacaccac cgacgctgtg agagacccac agaccctgga aatcctggac atcaccccct
     4861 gctctttcgg aggagtgagc gtgatcaccc caggaaccaa cacctccaac caggtggccg
     4921 tgctgtacca ggacgtgaac tgcaccgaag tgccagtggc catccacgct gaccagctga
     4981 ccccaacctg gagagtgtac agcaccggat ctaacgtgtt ccagacccgt gccggctgcc
     5041 tgatcggagc cgagcacgtg aacaactcct acgaatgcga catcccaatc ggagctggca
     5101 tctgcgcttc ttaccagacc cagaccaaca gcccaagaag ggccagatcc gtggcctctc
     5161 agagcatcat cgcctacacc atgagcctgg gcgctgaaaa cagcgtggct tacagcaaca
     5221 actctatcgc catcccaacc aacttcacca tctctgtgac caccgaaatc ctgccagtga
     5281 gcatgaccaa gaccagcgtg gactgcacca tgtacatctg cggagactct accgaatgct
     5341 ctaacctgct gctgcagtac ggcagcttct gcacccagct gaaccgcgct ctgaccggaa
     5401 tcgctgtgga gcaggacaag aacacccagg aagtgttcgc tcaggtgaag cagatataca
     5461 agaccccacc tatcaaggac ttcggaggat tcaacttctc tcagatcctg cccgacccat
     5521 ctaagccctc taagaggtct ttcatcgaag acctgctgtt caacaaggtg accctggccg
     5581 acgccggttt catcaagcag tacggtgact gcctgggaga catcgccgct agagacctga
     5641 tctgcgccca gaagttcaac ggtctgaccg tgctgccacc actgctgacc gacgagatga
     5701 tcgcccagta cacctctgcc ctgctggctg gaaccatcac ctctggttgg accttcggag
     5761 ctggcgccgc cctgcagatc ccattcgcta tgcagatggc ctaccgtttc aacggtatcg
     5821 gagtgaccca gaacgtgctg tacgaaaacc agaagctgat cgctaaccag ttcaactctg
     5881 ctatcggaaa gatccaggac tccctgtcct ctaccgcttc tgccctggga aagctgcagg
     5941 acgtggtgaa ccagaacgcc caggccctga acaccctggt gaagcagctg tcttctaact
     6001 tcggagccat ctcctctgtg ctgaacgaca tcctgtctag gctggacaag gtggaagccg
     6061 aggtgcagat cgacagactg atcaccggaa gactgcagtc tctgcagacc tacgtgaccc
     6121 agcagctgat cagggccgct gaaatcagag cttctgccaa cctggctgcc accaagatgt
     6181 ccgaatgcgt gctgggccag tctaagaggg tggacttctg cggaaaggga taccacctga
     6241 tgtccttccc acagtctgct ccacacggag tggtgttcct gcacgtgacc tacgtgcccg
     6301 cccaggaaaa gaacttcacc accgccccag ctatctgcca cgacggaaag gctcacttcc
     6361 ccagggaggg agtgttcgtg tctaacggaa cccactggtt cgtgacccag aggaacttct
     6421 acgagccaca gatcatcacc accgacaaca ccttcgtgtc tggaaactgc gacgtggtga
     6481 tcggaatcgt gaacaacacc gtgtacgacc cactgcagcc agaactggac tctttcaagg
     6541 aggaactgga caagtacttc aagaaccaca cctctccaga cgtggacctg ggagacatct
     6601 ccggaatcaa cgcctctgtg gtgaacatcc agaaggaaat cgacagactg aacgaggtgg
     6661 ccaagaacct gaacgaatct ctgatcgacc tgcaggaact gggaaagtac gagcagtaca
     6721 tcaagtggcc ctggtacatc tggctgggtt tcatcgctgg tctgatcgct atcgtgatgg
     6781 tgaccatcat gctgtgctgc atgacctctt gctgctcttg cctgaaggga tgctgctctt
     6841 gcggatcttg ctgcaagttc gacgaggacg actccgagcc tgtgctgaag ggtgtgaagc
     6901 tgcactacac ctacccaact ttcttgtaca aagtggttga tatcaaggcc gccgacatca
     6961 ccagcctgta caagaaagtg gggtggagcc atccccagtt cgagaagggc ggaggatctg
     7021 gcggcggaag cggcggagga agctggtccc accctcagtt tgagaaaggc accgagctgg
     7081 gcagcaccat ggccagctac ccctacgacg tgcccgacta cgcctgatga ctcgagtcta
     7141 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7201 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7261 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7321 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7381 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7441 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7501 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7561 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7621 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct
     7681 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa
     7741 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     7801 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     7861 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     7921 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7981 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     8041 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     8101 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     8161 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     8221 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     8281 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     8341 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     8401 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     8461 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     8521 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     8581 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     8641 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     8701 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     8761 acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8821 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8881 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9001 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     9061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9301 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9361 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9421 aaaaggccag gaaccgtaaa aaggccgcgt tgc
//
