LOCUS       sequence-384603-        5472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline 
            Spike extracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)
FEATURES             Location/Qualifiers
     source          1..5472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      130..718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     619..638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             893..922
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(4656..4690)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4761..5420)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5335..5354
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
       61 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      781 ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcata tggccttgcc
      841 cgttacggca cttctgctcc cactcgcatt gctgcttcac gccgcccgac cagaacagaa
      901 actcataagt gaagaagacc ttgggggagg cggttccggg ggaggcggtt ctagccagtg
      961 cgtgaacctg accacccgaa cccagctccc accagcctac accaacagct ttacacgggg
     1021 cgtgtactac cctgacaagg tgttcagatc tagcgtcctg cacagcactc aggacctctt
     1081 cctgccgttc ttcagcaacg tgacatggtt ccacgccatc cacgtgagcg gcacaaacgg
     1141 aaccaagcgg tttgataacc ccgtcctgcc attcaatgat ggagtttact tcgccagtac
     1201 cgagaagagt aacatcatcc ggggctggat cttcggcacc accctggata gcaaaacaca
     1261 gagcctcctg atcgtgaaca atgccacgaa cgtcgtgatc aaggtgtgcg agttccagtt
     1321 ttgcaatgat cctttcctgg gtgtgtacta ccacaagaac aacaagagct ggatggaaag
     1381 cgagttcaga gtctacagca gcgccaacaa ctgcacattc gagtatgtct ctcagccttt
     1441 tctgatggac cttgagggga aacaaggcaa cttcaagaac ctgagagaat tcgtgttcaa
     1501 gaacatcgac ggctacttca aaatctactc caagcacaca cccatcaacc tggtccggga
     1561 cctccctcag ggcttcagcg ccctggaacc cctggtcgac ctgcccatag gcatcaacat
     1621 aacgcggttc caaaccctgc tggccctgca tagatcctac ctgactcctg gcgacagcag
     1681 cagcggatgg accgccggag ctgcagccta ctatgtgggc tacctgcaac ctagaacctt
     1741 cctgctgaag tacaacgaga acggcacaat cacagacgcc gtcgactgcg ccctggaccc
     1801 tctctctgag acaaagtgca ccctgaagtc cttcaccgtg gaaaagggca tctaccagac
     1861 cagcaacttc cgggtgcagc ctacagagag catcgtgcga tttccaaaca ttaccaacct
     1921 ctgccccttc ggcgaggtgt ttaacgccac aagatttgcc tccgtttacg cctggaatag
     1981 aaagagaatc agcaattgtg tggccgacta ctccgtgctg tataacagcg cctctttcag
     2041 caccttcaag tgctacggcg tttccccaac aaagctgaat gacctgtgct tcaccaacgt
     2101 gtacgccgac tccttcgtaa ttagaggcga tgaggtgcgg cagatcgcac caggccagac
     2161 cggtaagatc gctgactaca actataagct gcctgatgat tttacaggct gcgtgatcgc
     2221 ctggaactct aacaacctgg atagcaaggt gggcggcaac tacaactacc tgtaccggct
     2281 gtttcgcaag tctaacctga aacctttcga gagagacatc tccacagaga tctaccaggc
     2341 cggttctaca ccttgtaacg gggtggaagg cttcaactgt tacttccctc tgcaaagcta
     2401 cggcttccag cctaccaatg gagtcggcta ccagccatac cgggtggtcg tgctgtcctt
     2461 cgagttactc cacgcccccg ccaccgtctg cggtcctaag aagtccacca atctggttaa
     2521 gaacaaatgc gtgaacttca acttcaacgg cctgaccggg accggcgtgc tgaccgaaag
     2581 caacaaaaag ttcctcccct tccagcagtt cggccgtgat atcgctgaca ccacagatgc
     2641 cgtcagagat ccacagaccc tggaaatcct ggatattaca ccctgctcct tcggaggagt
     2701 ttctgtgatc acccccggga ccaataccag caaccaggtg gctgtgctgt accaagatgt
     2761 taactgcacc gaggttcctg tggccatcca cgccgatcag ctgacaccta cttggagagt
     2821 gtactccact ggctccaatg tgttccagac cagggccgga tgtctgatcg gcgccgagca
     2881 cgtgaataac agttacgagt gcgacatccc tatcggcgcc ggcatctgtg ccagctacca
     2941 gacccagaca aacagccctg ggtctgcttc ctctgtagct agccagagca tcatcgccta
     3001 caccatgagc ctgggcgcag agaacagcgt ggcctattcc aacaactcta tcgccattcc
     3061 caccaacttt acaattagcg tcacaacaga gatcctgccc gtgagcatga ccaagaccag
     3121 cgtggactgt acaatgtaca tctgtggcga cagcactgaa tgcagcaacc tgctgctgca
     3181 atacggctcc ttttgcaccc aactgaaccg ggcgctgacc ggaatcgccg tggaacagga
     3241 caaaaatacc caggaggtgt tcgcccaagt gaagcagatc tacaagaccc cacctatcaa
     3301 ggacttcggc ggctttaact ttagccagat tctccctgat ccttctaagc ctagcaagcg
     3361 gagccctatc gaggatctgc tgttcaacaa ggtcaccctg gccgatgccg gctttatcaa
     3421 acagtatggc gattgcctgg gcgacatagc cgccagagat ctgatctgcg cccagaaatt
     3481 caacggcctg acagttctcc cacctctgct gaccgacgag atgatcgctc agtacacctc
     3541 tgccctgctg gctggcacca tcacatctgg gtggacattt ggcgccggcc ccgccctgca
     3601 gatccccttt cccatgcaga tggcctatag attcaacgga atcggcgtga cccagaacgt
     3661 gctgtatgaa aaccagaagc tgatcgctaa ccagttcaat tctgccatcg gcaagatcca
     3721 ggactccctc tcctctaccc ccagcgccct gggcaaactg caggacgtgg tgaatcagaa
     3781 cgcccaagcc ctgaacaccc tggtgaagca gctcagcagc aattttggcg ccatcagctc
     3841 tgtgctgaac gatatcctgt ctagactgga ccctccagaa gccgaagtcc agatcgatag
     3901 actgatcaca ggcagactgc agtccctgca aacctacgtg acccaacagc tgatcagggc
     3961 cgctgaaata agagccagcg ccaatctcgc cgctaccaag atgtccgagt gtgtgctggg
     4021 acagtctaaa cgcgttgact tctgcggcaa aggctatcac ctgatgagct tcccccagag
     4081 cgcgccgcac ggcgtggtgt tcctgcatgt gacatacgtg cctgcccaag agaagaattt
     4141 cacaaccgcc cctgccatct gccacgacgg caaggcccac ttcccaagag agggcgtttt
     4201 cgtttccaat ggcacacact ggttcgtgac acaaagaaac ttctacgaac cccagattat
     4261 caccaccgac aacaccttcg tgagtggcaa ttgtgacgtg gtcatcggaa tcgtgaacaa
     4321 cacagtgtac gaccctctgc aacctgagct ggactctttt aaggaagagc tggacaagta
     4381 ctttaaaaac cacaccagcc ccgatgtgga cctgggcgac atcagtggca ttaacgccag
     4441 cgtggtgaac atccaaaagg aaatcgacag actgaacgag gtggccaaga acctgaacga
     4501 gtccctgatc gacctgcagg agctcggcaa atacgagcag ggatccggat acatccccga
     4561 ggcccccaga gatggccagg cctacgtgcg gaaggacggc gagtgggtac tgctgagcac
     4621 attcctgggc agatccggtt cttgagacca gaccaataaa aaacgcccgg cggcaaccga
     4681 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     4741 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     4801 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     4861 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     4921 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     4981 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     5041 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     5101 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     5161 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     5221 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     5281 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     5341 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     5401 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     5461 aatacgcccg gt
//
