LOCUS       sequence-384679-        9257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: pb-miRE-tre-3xFLAG-hnRNPU∆RGG
FEATURES             Location/Qualifiers
     source          1..9257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1367..1681
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1375..1393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1411..1429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1446..1464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1482..1500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1518..1536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1553..1571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1589..1607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1654..1678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1720..1785
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    3754..3978
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4106..4196
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        4244..4455
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4468..4736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4773..5798
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5827..5948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5864..5883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5918..5937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6711..6745
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7236..7252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7236..7252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7249..7271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7260..7276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7284..7290,7291..7308,7309..7314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7329..7350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7467..7484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7638..8226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7718..7737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8397..9188,9189..9257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9020..9039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagttcgagt ttactcccta
     1381 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
     1441 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     1501 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     1561 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg
     1621 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1681 ctggaccggt cgtttagtga accgtcagaa ttaaccatgg actacaaaga ccatgacggt
     1741 gattataaag atcatgacat cgattacaag gatgacgatg acaagagcgg atccatgagt
     1801 tcttcgcctg ttaatgtcaa gaagctgaag gtgtcggagc tgaaggagga gctcaagaag
     1861 cggcgcctct ccgacaaggg cctcaaggcc gatctcatgg atcgactcca ggccgcgctg
     1921 gacaacgagg caggaggccg ccccgccatg gagcccggga acggcagtct cgacctaggt
     1981 ggcgatgcgg ccgggcgctc gggagcgggc ctagagcagg aggccgcggc tggcgccgaa
     2041 gacgacgagg aggaggaggg catcgccgct ctggacggcg accagatgga gctgggcgag
     2101 gagaacgggg cggcgggggc ggctgacgcc ggcgcgatgg aggaagagga ggcagcgtcg
     2161 gaagacgaga acggcgacga ccagggcttc caggaggggg aagacgagct cggcgacgag
     2221 gaggagggcg cgggcgacga gaacggtcac ggggagcagc agtcccaacc gccggcagcg
     2281 gcggcgcagc agcagccttc ccagcagcgt ggtgccggca aggaggccgc gggcaagagc
     2341 agcggcccca cctcgctctt cgcggtgacg gtggcgccgc caggggcgag gcagggccaa
     2401 cagcaggcgg gaggagacgg caaaacagaa cagaaaggtg gagataaaaa gagaggcgtt
     2461 aaaagaccgc gagaagatca tggccgaggg tattttgagt acatcgaaga aaacaagtac
     2521 agcagagcca agtctcctca gccacctgtt gaagaagaag acgaacactt cgatgacaca
     2581 gtggtttgtc ttgatactta taattgtgat ctgcatttta aaatctcgag agaccgtctg
     2641 agtgcttctt cccttactat ggagagtttt gctttcctgt gggctggagg aagagcttcc
     2701 tacggtgtgt caaaaggcaa agtctgcttt gagatgaagg taacagagaa gattccagta
     2761 agacacttat atacaaaaga tattgatata catgaagttc ggattggctg gtcactaacc
     2821 acaagtggaa tgttgcttgg tgaagaagaa ttttcttacg ggtattctct gaaaggaata
     2881 aaaacatgca actgtgagac agaagattat ggggagaagt ttgatgaaaa tgatgtgatt
     2941 acatgctttg ctaactttga aactgatgaa gttgaactct cttatgcgaa gaatggacaa
     3001 gatcttggtg ttgcctttaa gatcagtaag gaagttcttg ctgaccggcc actattccca
     3061 catgttctct gccataactg tgcagttgaa tttaatttcg gtcaaaagga aaagccatac
     3121 tttccaatac ctgaagactg tacttttatc caaaatgtcc ccttagagga ccgagttaga
     3181 ggaccaaaag gacctgaaga gaagaaggat tgtgaggttg taatgatgat tggcttgcca
     3241 ggagctggaa aaactacctg ggttactaaa catgcagctg aaaaccctgg gaaatacaac
     3301 attcttggaa caaatacgat tatggacaag atgatggtgg caggttttaa gaagcaaatg
     3361 gcagatactg gaaaactgaa cacactgttg cagagagccc cacagtgtct tggcaagttt
     3421 attgaaattg ctgcccgtaa gaagcgaaat tttattctgg atcagacaaa tgtgtctgct
     3481 gctgcccaga gaagaaaaat gtgcctgttt gcaggcttcc agcggaaagc tgttgtagtg
     3541 tgcccaaaag atgaagacta taagcagagg acacagaaga aggcagaagt agaggggaag
     3601 gacctaccag aacatgctgt cctcaagatg aaaggaaact tcacccttcc agaggttgca
     3661 gaatgctttg atgaaataac ctatgttgaa cttcagaaag aggaagccca aaagcttttg
     3721 gagcaatata aatgagtcga caatgcgttt cgactgtgcc ttctagttgc cagccatctg
     3781 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3841 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3901 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3961 atgcggtggg ctctatggaa acacgcggcg acgcgttcga ccgaataaaa cctgtgacgg
     4021 aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg aagccctggg
     4081 ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata
     4141 atgaaataag atcactaccg ggcgtatttt ttgagttgtc gagattttca ggagctaaag
     4201 atctggaagc taaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4261 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4321 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4381 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4441 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4501 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4561 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     4621 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     4681 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     4741 ctgtgaccgg cgcctacaag ctgctagcca ccatgaaaaa gcctgaactc accgcgacgt
     4801 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     4861 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     4921 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     4981 ccgcgctccc gattccggaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt
     5041 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     5101 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     5161 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     5221 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     5281 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     5341 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     5401 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     5461 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     5521 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     5581 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     5641 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     5701 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     5761 accgacgccc cagcactcgt ccgagggcaa aggaatagtt ttacgtattg agtatttaac
     5821 taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5941 gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc
     6001 tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa
     6061 actgggttct tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag
     6121 gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc
     6181 ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca
     6241 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
     6301 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
     6361 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
     6421 ggggggggct gtccctgata tctataacaa gaaaatatat atataataag ttatcacgta
     6481 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     6541 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     6601 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     6661 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     6721 ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc
     6781 ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc
     6841 ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg
     6901 ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat
     6961 gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct
     7021 tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg
     7081 cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata
     7141 ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg
     7201 tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7261 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7321 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7381 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7441 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8041 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8701 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9001 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9241 aaatgttgaa tactcat
//
