LOCUS       sequence-384691-       10464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Yu G, Kim HK, Park J, Kwak H, Cheong Y, Kim D, Kim J, Kim J, Kim HH
  TITLE     Prediction of efficiencies for diverse prime editing systems in 
            multiple cell types.
  JOURNAL   Cell. 2023 Apr 21:S0092-8674(23)00331-8. doi: 
            10.1016/j.cell.2023.03.034.
  PUBMED    37119812
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Apr 21:S0092-8674(23)00331-8. doi: 10.1016/j.cell.2023.03.034."
COMMENT     Sequence Label: pLenti-EF1a-hMLH1dn-Hygro
FEATURES             Location/Qualifiers
     source          1..10464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     228..248
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        230..248
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        276..502
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             503..683
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    730..855
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1348..1581
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1764..1881
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2020..3198
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2251..3189
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3146..3166
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5494..5550
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5551..6576
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    6592..7180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6645..6665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7063..7074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7252..7485
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7563..7684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7600..7619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7654..7673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7724..7859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7879..7898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7880..7898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7908..7925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7908..7924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7917..7939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8066..8521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8153..8172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8363..8384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8547..8651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8652..8720,8721..9512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8870..8889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9683..10271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10172..10191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10425..10442
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
      241 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
      301 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
      361 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
      421 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
      481 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
      541 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      601 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      661 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      721 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      781 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      841 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      901 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      961 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1021 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1081 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1141 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1201 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1261 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1321 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1381 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1441 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1501 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1561 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1621 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1681 gacctggatg gagtggggca tatgacatgg gatgaagctt ggcgtaacta gatcttgaga
     1741 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     1801 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     1861 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     1921 cggctcgagg gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     1981 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2041 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     2101 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2161 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2221 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     2281 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     2341 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     2401 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     2461 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     2521 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     2581 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     2641 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     2701 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     2761 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     2821 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     2881 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     2941 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3001 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3061 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     3121 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     3181 ccatttcagg tgtcgtgacg tacgggatcc tctagagcca ccatgagctt cgttgctgga
     3241 gtcatccgga gactggacga gacagtggtg aacagaattg ccgccggcga ggtgatccag
     3301 agacctgcca atgcaatcaa ggagatgatc gagaactgtc tggacgccaa gtccacaagc
     3361 attcaggtga tcgtgaagga gggcggactg aagctgatcc agatccaaga caacggcaca
     3421 ggcatcagaa aggaagatct ggacatcgtg tgtgaacggt tcaccacatc taagctgcag
     3481 tcttttgagg atctggcctc tatcagtacc tacggcttca gaggcgaggc cctggccagc
     3541 atcagccacg tggcccatgt gaccatcacc accaaaaccg ccgacggcaa atgcgcttat
     3601 cgcgctagct acagcgacgg caagctgaaa gccccgccaa agccttgcgc cggcaaccag
     3661 ggtacacaga taacagtgga ggatctgttc tacaacatcg ccacccggag aaaggccctg
     3721 aaaaatccca gcgaggagta cggcaagatc ctggaagtcg tcggcagata ctccgtgcac
     3781 aacgccggaa tcagctttag cgtaaagaag cagggagaaa ccgtggccga tgtgcgcacc
     3841 ctgccaaatg ccagcaccgt ggataacatc agaagcattt tcggaaatgc cgtgtccaga
     3901 gaactgatcg agatcggctg cgaagataag accctggctt ttaagatgaa cggctacatc
     3961 agcaacgcca attactctgt gaagaagtgc atctttcttc tgttcatcaa ccacagactg
     4021 gtggaaagca ccagcctgcg gaaagccatc gagacagtgt acgccgccta cctgcctaag
     4081 aacacccacc ccttcctgta cctgagcctc gagatcagcc ctcagaacgt ggacgtcaat
     4141 gtgcatccta caaagcacga ggtgcacttc ctgcacgagg aaagcatcct ggaaagagtg
     4201 cagcagcaca ttgagagcaa gctgctgggc tctaacagca gcagaatgta cttcacacag
     4261 accctgctgc ctggcctggc cggcccctca ggcgaaatgg ttaagtccac aacctctctg
     4321 acctcatcca gcaccagcgg ttcttccgat aaggtgtacg cccaccagat ggtgcggacc
     4381 gactctcggg agcagaagct ggacgccttt ctgcaacctc tgagcaaacc tctgagctct
     4441 cagcctcagg ccatcgtgac cgaggacaag acagatatct cctccggccg tgccagacag
     4501 caggacgaag aaatgctcga gctgccagct cctgccgagg tggccgccaa gaaccagagc
     4561 ctggagggag ataccacaaa gggcaccagc gaaatgagcg agaagcgggg ccctacctcc
     4621 agcaacccca gaaaacggca ccgggaggac agcgacgtgg aaatggtgga ggacgacagc
     4681 agaaaggaaa tgacagccgc ttgtacccct agaagaagaa tcatcaacct gacctccgtg
     4741 ctgagcctgc aggaggagat caacgagcag ggccacgagg tgctgagaga gatgctgcac
     4801 aatcacagct tcgtgggctg cgtgaaccct caatgggccc tggctcagca tcaaacaaag
     4861 ctgtacctgc tgaacaccac caagctgagc gaagagctgt tctaccagat cctcatctac
     4921 gacttcgcca acttcggcgt gctacgcctg agcgagcccg cccctctgtt tgacctggcc
     4981 atgctggctc tggatagccc agaaagcggc tggacagaag aggacggacc taaagagggg
     5041 ctggctgaat acatcgtgga gttcctgaag aaaaaggccg agatgctggc cgactacttt
     5101 tctctggaaa tcgacgagga aggcaacctg atcggcctgc ctctgctgat cgataactac
     5161 gtgcctcccc tggaaggcct gcccatcttc atcctgagac tggctacaga ggtgaactgg
     5221 gacgaggaaa aggaatgctt cgagtctctg agcaaggagt gcgccatgtt ctatagcatc
     5281 agaaaacagt acatctctga agagagcact ctgtctggcc agcagagtga agtgcccgga
     5341 agcatcccca acagctggaa gtggaccgtg gaacacatcg tgtacaaggc cctgcggagc
     5401 cacattctcc ctcctaagca cttcaccgag gacggcaaca tcctgcagct ggccaacctg
     5461 cccgaccttt ataaggtttt caccggttcc ggcgcaacaa acttctctct gctgaaacaa
     5521 gccggagatg tcgaagagaa tcctggaccg atgaaaaagc ctgaactcac cgcgacgtct
     5581 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     5641 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     5701 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5761 gcgctcccga ttccggaagt gcttgacatt ggggagttca gcgagagcct gacctattgc
     5821 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     5881 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     5941 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     6001 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6061 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     6121 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     6181 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     6241 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     6301 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     6361 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     6421 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     6481 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     6541 cgacgcccca gcactcgtcc gagggcaaag gaataaacgc gttaagtcga caatcaacct
     6601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6961 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7141 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     7201 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7261 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7321 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7381 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7441 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     7501 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7561 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7621 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7681 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7741 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7801 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7861 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     7921 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7981 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8041 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8101 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8161 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8221 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8281 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8341 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8401 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8461 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8521 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8581 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8641 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8701 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8761 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8821 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8881 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8941 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9001 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9061 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9121 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9181 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9241 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9301 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9361 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9421 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9481 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9541 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9601 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10381 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10441 aggaagcgga agagcgccca atac
//
