LOCUS       sequence-38481-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pAB07 (CK2beta)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     172..188
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     172..188
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             205..234
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      121 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctctagaact
      181 agtggatcca ccatggcgtc tatggaacaa aagctgatta gcgaagagga cctgaacaac
      241 gggaattctc gagcagccgc tgacgtgaag atgagcagct cagaggaggt gtcctggatt
      301 tcctggttct gtgggctccg tggcaatgaa ttcttctgtg aagtggatga agactacatc
      361 caggacaaat ttaatcttac tggactcaat gagcaggtcc ctcactatcg acaagctcta
      421 gacatgatct tggacctgga gcctgatgaa gaactggaag acaaccccaa ccagagtgac
      481 ctgattgagc aggcagccga gatgctttat ggattgatcc acgcccgcta catccttacc
      541 aaccgtggca tcgcccagat gttggaaaag taccagcaag gagactttgg ttactgtcct
      601 cgtgtgtact gtgagaacca gccaatgctt cccattggcc tttcagacat cccaggtgaa
      661 gccatggtga agctctactg ccccaagtgc atggatgtgt acacacccaa gtcatcaaga
      721 caccatcaca cggatggcgc ctacttcggc actggtttcc ctcacatgct cttcatggtg
      781 catcccgagt accggcccaa gagacctgcc aaccagtttg tgcccaggct ctacggtttc
      841 aagatccatc cgatggccta ccagctgcag ctcc
//
