LOCUS       sequence-384901-        5706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent 
            protein miRFP718nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Oliinyk OS, Ma C, Pletnev S, Baloban M, Toboada C, Sheng H, Yao J, 
            Verkhusha VV
  TITLE     Deep-tissue SWIR imaging using rationally designed small red-shifted
            near-infrared fluorescent protein.
  JOURNAL   Nat Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0.
  PUBMED    36456785
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0."
COMMENT     Sequence Label: pLAMP1-miRFP718nano
FEATURES             Location/Qualifiers
     source          1..5706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        114..417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        418..621
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     572..592
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      677..686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(679..741,742..1899)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     polyA_signal    2547..2668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2584..2603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2638..2657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2675..3130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2812..2833)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3024..3043
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3157..3261
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3263..3620
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3263..3454
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3286..3306)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3471..3606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3655..4449
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3655..4449
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3709..3728)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4319..4338
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4637..4656)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4681..4728
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5057..5645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5546..5565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
       61 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      121 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      181 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      241 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      301 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      361 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      421 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      481 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      541 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      601 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      661 cagatctcga gcgccaccat ggcggccccg ggcgcccggc gtccgctgct cctgttgctg
      721 ctggcaggcc ttgcacacag cgccccagca ctgttcgagg tgaaagacaa caacggcaca
      781 gcgtgtataa tggccagctt ctctgcctcc tttctgacca cctatgaggc tggacatgtt
      841 tctaaggtct cgaatatgac cctgccagcc tctgcagaag tcctgaagaa tagcagctct
      901 tgtggtgaaa agaatgcttc tgagcccacc ctcgcaatca cctttggaga aggatattta
      961 ctgaaactca ccttcacaaa aaacacaaca cgttacagtg tccagcacat gtatttcaca
     1021 tataacctgt cagacacaca attctttccc aatgccagct ccaaagggcc cgacactgtg
     1081 gattccacaa ctgacatcaa ggcagacatc aacaaaacat accgatgtgt cagcgacatc
     1141 agggtctaca tgaagaatgt gaccattgtg ctctgggacg ctactatcca ggcctacctg
     1201 ccgagtagca acttcagcaa ggaagagaca cgctgcccac aggatcaacc ttccccaact
     1261 actgggccac ccagcccctc accaccactt gtgcccacaa accccagtgt gtccaagtac
     1321 aatgtgactg gtgacaatgg aacctgcctg ctggcctcta tggcactgca actcaacatc
     1381 acctacatga agaaggacaa cacgactgtg accagagcat tcaacatcaa cccaagtgac
     1441 aaatatagtg ggacttgcgg tgcccagttg gtgaccctga aggtggggaa caagagcaga
     1501 gtcctggagc tgcagtttgg gatgaatgcc acttctagcc tgtttttcct gcaaggagtt
     1561 cagttgaaca tgactcttcc tgatgccata gagcccacgt tcagcacctc caactattcc
     1621 ctgaaagctc ttcaggccag tgtcggcaac tcatacaagt gcaacagtga ggagcacatc
     1681 tttgtcagca aggcgctcgc cctcaatgtc ttcagcgtgc aagtccaggc tttcagggta
     1741 gaaagtgaca ggtttgggtc tgtggaagag tgtgtacagg acggtaacaa catgctgatc
     1801 cccattgctg tgggcggggc cctggcaggg ctggtcctca tcgtcctcat cgcctacctc
     1861 atcggcagga agaggagtca cgcgggctat cagaccatct cggaattcgg ctccaccggc
     1921 tccaccggct ccaccggcgc ggatccaccg gtaatggcaa acctggacaa gatgctgaac
     1981 accatcgtga ccgaggtgcg ccagttcctg caagtggaca gactctgcgt gttcaagttc
     2041 gaggaagatt actccggcaa catcatctac gaagccgtgg acgacggatg gttgagcatc
     2101 ctgaagaccc acgtgcggga ctgctacttc atggaaacca gaggcgagga atacctgcac
     2161 ggcagatacc aggccatcgc cgacatccac caggccaatc tggcggagag ctatagagac
     2221 ttcctgaccc agtaccaggt gcgggccatt gtggctgtcc ccatcctgaa gggcaagaag
     2281 ctgtggggct tgttcagcgc ccaccaactg gccgcccctc ggagctggca ggcctgggaa
     2341 atcgagttcc tgaaacagca agccgtggtg atgggcatcg ccatccagca gagctagctg
     2401 aattctgcag atatccagca cagtggcggc cgcgactcta gatcataatc agccatacca
     2461 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2521 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2581 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2641 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     2701 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     2761 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     2821 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     2881 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     2941 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3001 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3061 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3121 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3241 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3301 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3361 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3421 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3541 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3601 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     3661 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     3721 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     3781 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     3841 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     3901 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     3961 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4021 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4081 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4141 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4201 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4261 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4321 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4381 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4441 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     4501 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4561 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4621 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4681 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     4741 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4801 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4861 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     4921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5701 acatgt
//
