LOCUS       sequence-385187-        3879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BORF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BORF1
FEATURES             Location/Qualifiers
     source          1..3879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1673..1684)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1758..1857)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1874..1893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1875..1893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1898..1914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1898..1914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2345..3136
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3229..3817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3718..3737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg aaggtccagg ggtccgtcga tcgccgccgt ctgcaacgcc gaatcgcggg
      721 gctgctgccc cctccggccc ggcgtctaaa tatttcccgg gggtccgaat tcacgcggga
      781 cgttcgtggg ctggttgagg aacacgcgca ggcctcctcg ctgagtgcgg cggccgtctg
      841 gcgcgcaggg ctgctggccc cgggggaggt ggcggtcgcc gggggtggca gtggaggggg
      901 gagcttcagc tggtctgggt ggcggccgcc agtctttggg gactttctga tacacgccag
      961 ctccttcaac aacgccgagg ccactggaac gccccttttc caattcaagc agagtgaccc
     1021 gttctcgggc gtcgacgcgg tattcactcc tctctccctg tttatcctaa tgaatcacgg
     1081 ccggggtgta gccgcccggg tcgaggcagg tgggggcctg acgcggatgg ccaacctgct
     1141 gtacgacagc cccgcaaccc tggctgacct ggtcccggac tttgggcggc tggtggccga
     1201 ccgccgcttc cacaacttca tcacccctgt gggccccctg gtggagaata taaagagcac
     1261 ctatctgaat aaaatcacca cggtggtcca cgggcctgtg gtcagcaagg ccatccctcg
     1321 cagcaccgtc aaggtgacgg tgccccagga ggcctttgtg gatctggacg cgtggctctc
     1381 cggcggcgcc gggggtggcg gtggagtatg cttcgtcggg gggctgggcc tgcagccgtg
     1441 ccccgccgat gcgcgcctct atgtcgctct gacctatgag gaagccgggc cgcggtttac
     1501 gtttttccag tcgtcccgcg gccactgtca gatcatgaat atcttaagaa tttattactc
     1561 accatccatc atgcaccgct atgctgtggt ccagccccta catatagagg agctaacctt
     1621 cggggcggtt gcctgtctgg ggacatttag tgctactgac ggttggagga ggtctgcctt
     1681 caattaccgt ggctctagcc tccccgtggt ggagattgac agcttttatt ccaacgtctc
     1741 tgactgggag gtgattctcc caactttctt gtacaaagtt ggcattataa gaaagcattg
     1801 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     1861 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     1921 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1981 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2041 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2101 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2161 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2221 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2281 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2341 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2401 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2461 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     2521 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2581 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     2641 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     2701 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     2761 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     2821 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     2881 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     2941 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3001 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3061 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3121 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3181 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3361 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3601 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3841 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
