LOCUS       sequence-38523-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pAB46 (CK2beta)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggctagc atgactggtg gacagcaaat
      121 gggtcgcgga tccgaattcg agctccgtcg acaagcttgc ggccgcagca gctcagagga
      181 ggtgtcctgg atttcctggt tctgtgggct ccgtggcaat gaattcttct gtgaagtgga
      241 tgaagactac atccaggaca aatttaatct tactggactc aatgagcagg tccctcacta
      301 tcgacaagct ctagacatga tcttggacct ggagcctgat gaagaactgg aagacaaccc
      361 caaccagagt gacctgattg agcaggcagc cgagatgctt tatggattga tccacgcccg
      421 ctacatcctt accaaccgtg gcatcgccca gatgttggaa aagtaccagc aaggagactt
      481 tggttactgt cctcgtgtgt actgtgagaa ccagccaatg cttcccattg gcctttcaga
      541 catcccaggt gaagccatgg tgaagctcta ctgccccaag tgcatggatg tgtacacacc
      601 caagtcatca agacaccatc acacggatgg cgcctacttc ggcactggtt tccctcacat
      661 gctcttcatg gtgcatcccg agtaccggcc caagagacct gccaaccagt ttgtgcccag
      721 gctctacggt ttcaagatcc atccgatggc ctaccagctg cagctccaag ccgccagcaa
      781 cttcaagagc ccagtcaaga cgattcgctg attccctccc ccacctgtcc tgcagtctgg
      841 atcccccggg ctgcaggaat tcgatatcaa gcttggtacc gagctcgatc
//
