LOCUS       sequence-38580-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pBSHSP:FLP;CMV:tdTomato-SceI
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..52
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     34..51
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(224..242)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     492..511
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     686..709
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc
       61 tacttgttct ttttgcagga tcccgccacc atggtgagca agggcgagga ggtcatcaaa
      121 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
      181 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      241 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
      301 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
      361 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
      421 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
      481 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
      541 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
      601 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
      661 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
      721 atcgtggaac agtacgagcg ctccgagggc cgccaccacc
//
