LOCUS       sequence-385936-       10321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0276
FEATURES             Location/Qualifiers
     source          1..10321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3259..3847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3312..3332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3730..3741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3919..4152
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4230..4351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4267..4286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4321..4340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4391..4526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4546..4565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4547..4565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4575..4592)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4575..4591)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4584..4606)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4733..5188
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4820..4839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5030..5051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5214..5318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5319..5387,5388..6179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5537..5556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6350..6938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6839..6858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7092..7109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7226..7247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7262..7267,7268..7285,7286..7292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7300..7316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7305..7327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7324..7340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7324..7340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7359..7379
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7361..7379
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7407..7633
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7634..7814
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7861..7986
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8479..8712
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8897..8941
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9172..9382
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9194..9390
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9241..9376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9422..10024
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9422..9441)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9918..9938
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10067..10184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ccagccgcag aggatggcgc ctgtgggcac agacaaggag ctcagtgacc tcctggactt
     1261 cagcatgatg ttcccgctgc ctgtcaccaa cgggaagggc cggcccgcct ccctggccgg
     1321 ggcgcagttc ggaggttcag gtcttgagga ccggcccagc tcaggctcct ggggcagcgg
     1381 cgaccagagc agctcctcct ttgaccccag ccggaccttc agcgagggca cccacttcac
     1441 tgagtcgcac agcagcctct cttcatccac attcctggga ccgggactcg gaggcaagag
     1501 cggtgagcgg ggcgcctatg cctccttcgg gagagacgca ggcgtgggcg gcctgactca
     1561 ggctggcttc ctgtcaggcg agctggccct caacagcccc gggcccctgt ccccttcggg
     1621 catgaagggg acctcccagt actacccctc ctactccggc agctcccggc ggagagcggc
     1681 agacggcagc ctagacacgc agcccaagaa ggtccggaag gtcccgccgg gtcttccatc
     1741 ctcggtgtac ccacccagct caggtgagga ctacggcagg gatgccaccg cctacccgtc
     1801 cgccaagacc cccagcagca cctatcccgc ccccttctac gtggcagatg gcagcctgca
     1861 cccctcagcc gagctctgga gtcccccggg ccaggcgggc ttcgggccca tgctgggtgg
     1921 gggctcatcc ccgctgcccc tcccgcccgg tagcggcccg gtgggcagca gtggaagcag
     1981 cagcacgttt ggtggcctgc accagcacga gcgtatgggc taccagctgc atggagcaga
     2041 ggtgaacggt gggctcccat ctgcatcctc cttctcctca gcccccggag ccacgtacgg
     2101 cggcgtctcc agccacacgc cgcctgtcag cggggccgac agcctcctgg gctcccgagg
     2161 gaccacagct ggcagctccg gggatgccct cggcaaagca ctggcctcga tctactcccc
     2221 ggatcactca agcaataact tctcgtccag cccttctacc cccgtgggct ccccccaggg
     2281 cctggcagga acgtcacagt ggcctcgagc aggagccccc ggtgccttat cgcccagcta
     2341 cgacgggggt ctccacggcc tgcagagtaa gatagaagac cacctggacg aggccatcca
     2401 cgtgctccgc agccacgccg tgggcacagc cggcgacatg cacacgctgc tgcctggcca
     2461 cggggcgctg gcctcaggtt tcaccggccc catgtcactg ggcgggcggc acgcaggcct
     2521 ggttggaggc agccaccccg aggacggcct cgcaggcagc accagcctca tgcacaacca
     2581 cgcggccctc cccagccagc caggcaccct ccctgacctg tctcggcctc ccgactccta
     2641 cagtgggcta gggcgagcag gtgccacggc ggccgccagc gagatcaagc gggaggagaa
     2701 ggaggacgag gagaacacgt cagcggctga ccactcggag gaggagaaga aggagctgaa
     2761 ggccccccgg gcccggacca gcagtacgga cgaggtgctg tccctggagg agaaagacct
     2821 gagggaccgg gagaggcgca tggccaataa cgcgcgggag cgggtgcgcg tgcgggatat
     2881 taacgaggcc ttccgggagc tggggcgcat gtgccagatg cacctcaagt cggacaaagc
     2941 gcagaccaag ctgctcatcc tgcagcaggc cgtgcaggtc atcctggggc tggagcagca
     3001 ggtgcgagag cggaacctga atcccaaagc agcctgtttg aaacggcgag aagaggaaaa
     3061 ggtgtcaggt gtggttggag acccccagat ggtgctttca gctccccacc caggcctgag
     3121 cgaagcccac aaccccgccg ggcacatgta gtaatgaact agtccgtaac ttgaaagtat
     3181 ttcgatttct tggctttata tatcttgtgg aaaggacgag actcacgcat tgactcagaa
     3241 tctacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3301 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3361 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3421 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3481 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3541 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3601 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3661 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3721 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3781 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3841 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3901 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3961 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4021 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4081 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4141 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4201 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4321 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4381 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4441 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4501 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4561 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4621 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4681 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4741 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4801 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4861 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4921 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4981 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5041 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5101 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5161 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5221 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5281 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5341 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5401 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5461 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5521 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5581 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5641 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5701 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5761 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5821 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5881 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5941 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6001 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6061 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6121 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6181 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6241 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6301 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6361 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6421 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6481 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6541 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6601 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6661 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6721 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6781 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6841 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6901 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6961 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7021 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7081 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7141 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7201 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7261 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7321 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7381 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7441 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7501 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7561 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7621 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7681 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7741 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7801 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7861 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7921 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7981 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8041 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8101 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8161 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8221 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8281 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8341 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8401 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8461 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8521 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8581 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8641 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8701 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8761 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8821 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8881 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8941 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9001 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9061 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9121 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9181 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9241 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9301 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9361 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9421 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9481 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9541 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9601 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9661 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9721 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9781 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9841 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9901 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9961 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    10021 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10081 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10141 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10201 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    10261 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10321 t
//
