LOCUS       sequence-38607-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-ntH6-eGFP
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(53..70)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(join(120..126,127..144,145..150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    165..186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      248..291
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(295..313)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        381..485
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(704..723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atatggctgc cgcgcggcac caggccgctg ctgtgatgat
       61 gatgatgatg gctgctgccc atagatctcc tccttctact agacgctcac aattccacac
      121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gtctagataa gttcaggatg
      241 aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg
      301 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      361 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      421 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      481 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      541 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      601 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      661 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      721 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      781 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      841 aattatgcag tgctgccata accatgagtg ataacactgc ggcc
//
