LOCUS       sequence-386197-       13465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mCherry tagged B1 Integrin. Wild-type..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Paszek MJ, Zahir N, Johnson KR, Lakins JN, Rozenberg GI, Gefen A, 
            Reinhart-King CA, Margulies SS, Dembo M, Boettiger D, Hammer DA, 
            Weaver VM
  TITLE     Tensional homeostasis and the malignant phenotype.
  JOURNAL   Cancer Cell. 2005 Sep;8(3):241-54. doi: 10.1016/j.ccr.2005.08.010.
  PUBMED    16169468
REFERENCE   2  (bases 1 to 13465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccr.2005.08"; journalName: "Cancer Cell"; date: 
            "2005-09"; volume: "8"; issue: "3"; pages: "241-54"
COMMENT     Sequence Label: B1-INTEGRIN WT
FEATURES             Location/Qualifiers
     source          1..13465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    73..190
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    join(2692..3266,3267..3269,3270..3278)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2859..2876)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3086..3105
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3279..3989
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3424..3442)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3692..3711
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3998..4586
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4051..4071)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4469..4480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4730..4763)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4783..4963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4988..5006)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4989..5006)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5296..5315)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5529..5548)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5648..5670
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5708..5726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5794..5898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5899..5967,5968..6759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6117..6136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6930..7518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7419..7438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7672..7689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7759..7779)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7764..8093
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7944..8079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9227..9292
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9422..9442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9867..10001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9904..9923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9958..9977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10169..10802
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10849..10974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11467..11700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11885..11929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(12312..13465,1..25)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(12543..13465,1..16)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     13438..13458
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg
       61 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      121 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      181 tcaaaatttt atcgatcacg agactagcct cgaggtttaa acttaagctt ggtaccgagc
      241 tcggatccgc caccatgaat ttacaaccaa ttttctggat tggactgatc agttcagttt
      301 gcggtgtgtt tgctcaaaca gatgaaaata gatgtttaaa agcaaatgcc aaatcatgtg
      361 gagaatgtat acaagcaggg ccaaattgtg ggtggtgcac aaattcaaca tttttacagg
      421 aaggaatgcc tacttctgca cgatgtgatg atttagaagc cttaaaaaag aagggttgcc
      481 ctccagatga catagaaaat cccagaggct ccaaagatat aaagaaaaat aaaaatgtaa
      541 ccaaccgtag caaaggaaca gcagagaagc tcaagccaga ggatattact cagatccaac
      601 cacagcagtt ggttttgcga ttaagatcag gggagccaca gacatttaca ttaaaattca
      661 agagagctga agactatccc attgacctct actaccttat ggacctgtct tattcaatga
      721 aagacgattt ggagaatgta aaaagtcttg gaacagatct gacgaatgaa atgaggagga
      781 ttacttcgga cttcagaatt ggatttggct catttgtgga aaagactgtg atgccttaca
      841 ttagcacaac accagctaag ctcaggaacc cttgcacaag tgaacagaac tgcaccagcc
      901 catttagcta caaaaatgtg ctcagtctta ctagtaaagg agaagtattt aatgaacttg
      961 ttggaaaaca gcgcatatct ggaaatttgg attctccaga aggtggtttc gatgccatca
     1021 tgcaagttgc agtttgtgga tcactgattg gctggaggaa tgttacacgg ctgctggtgt
     1081 tttccacaga tgccgggttt cactttgctg gagatgggaa acttggtggc attgttttac
     1141 caaatgatgg acaatgtcac ctggaaaata atatgtacac aatgagccat tattatgatt
     1201 atccttctat tgctcacctt gtccagaaac tgagtgaaaa taatattcag acaatttttg
     1261 cagttactga agaatttcag cctgtttaca aggagctgaa aaacttgatc cctaagtcag
     1321 cagtaggaac attatctgcc aattctagca atgtaattca gttgatcatt gatgcataca
     1381 attccctttc ctcagaagtc attttggaaa acggcaaatt gtcagaaggc gtaacaataa
     1441 gttacaaatc ttactgcaag aacggggtga atggaacagg ggaaaatgga agaaaatgtt
     1501 ccaatatttc cattggagat gaggttcaat ttgaaattag cataacttca aataagtgtc
     1561 caaaaaagga ttctgacagc tttaaaatta ggcctctggg ctttacggag gaagtagagg
     1621 ttattcttca gtacatctgt gaatgtgaat gccaaagcga aggcatccct gaaagtccca
     1681 agtgtcatga aggaaatggg acatttgagt gtggcgcgtg caggtgcaat gaagggcgtg
     1741 ttggtagaca ttgtgaatgc agcacagatg aagttaacag tgaagacatg gatgcttact
     1801 gcaggaaaga aaacagttca gaaatctgca gtaacaatgg agagtgcgtc tgcggacagt
     1861 gtgtttgtag gaagagggat aatacaaatg aaatttattc tggcaaattc tgcgagtgtg
     1921 ataatttcaa ctgtgataga tccaatggct taatttgtgg aggaaatggt gtttgcaagt
     1981 gtcgtgtgtg tgagtgcaac cccaactaca ctggcagtgc atgtgactgt tctttggata
     2041 ctagtacttg tgaagccagc aacggacaga tctgcaatgg ccggggcatc tgcgagtgtg
     2101 gtgtctgtaa gtgtacagat ccgaagtttc aagggcaaac gtgtgagatg tgtcagacct
     2161 gccttggtgt ctgtgctgag cataaagaat gtgttcagtg cagagccttc aataaaggag
     2221 aaaagaaaga cacatgcaca caggaatgtt cctattttaa cattaccaag gtagaaagtc
     2281 gggacaaatt accccagccg gtccaacctg atcctgtgtc ccattgtaag gagaaggatg
     2341 ttgacgactg ttggttctat tttacgtatt cagtgaatgg gaacaacgag gtcatggttc
     2401 atgttgtgga gaatccagag tgtcccactg gtccagacat cattccaatt gtagctggtg
     2461 tggttgctgg aattgttctt attggccttg cattactgct gatatggaag cttttaatga
     2521 taattcatga cagaagggag tttgctaaat ttgaaaagga gaaaatgaat gccaaatggg
     2581 acacgggtga aaatcctatt tataagagtg ccgtaacaac tgtggtcaat ccgaagtatg
     2641 agggaaaatg agtactcgag tctagagggc ccgtttaaac ccgctgatcc gcccctctcc
     2701 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     2761 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     2821 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     2881 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     2941 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3001 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3061 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3121 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
     3181 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     3241 tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagg
     3301 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3361 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3421 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3481 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     3541 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3601 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3661 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     3721 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     3781 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     3841 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     3901 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     3961 ccggcggcat ggacgagctg tacaagtaac atatgataat caacctctgg attacaaaat
     4021 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4081 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4141 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4201 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4261 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4321 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4381 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4441 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4501 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4561 gatctccctt tgggccgcct ccccgcatcg gtacgtatgg ccaggtacct ttaagaccaa
     4621 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4681 ggctaattca ctcccaacga agacaagatg ggatcaattc accatgggaa taacttcgta
     4741 tagcatacat tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac
     4801 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4861 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4921 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
     4981 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
     5041 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
     5101 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
     5161 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
     5221 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     5281 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     5341 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     5401 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     5461 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     5521 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5581 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5641 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5701 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5761 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5881 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5941 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6001 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6061 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6121 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6421 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7681 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7741 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7801 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7861 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7921 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7981 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8041 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     8101 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     8161 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     8221 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     8281 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     8341 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     8401 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     8461 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     8521 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     8581 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     8641 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     8701 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     8761 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     8821 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     8881 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     8941 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     9001 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     9061 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     9121 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     9181 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     9241 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     9301 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     9361 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     9421 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     9481 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     9541 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     9601 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     9661 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     9721 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     9781 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     9841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     9901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9961 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    10021 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    10081 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    10141 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    10201 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    10261 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    10321 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    10381 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    10441 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    10501 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    10561 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    10621 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10741 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10801 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    10861 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    10921 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    10981 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    11041 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    11101 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    11161 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    11221 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    11281 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    11341 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    11401 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    11461 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    11521 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    11581 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    11641 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    11701 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    11761 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    11821 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    11881 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    11941 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    12001 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    12061 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
    12121 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
    12181 acggtatcga tcacgagact agcctcgacc atcgatgtcg acgataagct ttgcaaagat
    12241 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
    12301 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
    12361 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
    12421 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
    12481 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
    12541 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
    12601 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
    12661 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
    12721 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
    12781 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
    12841 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
    12901 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
    12961 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
    13021 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
    13081 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
    13141 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
    13201 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
    13261 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
    13321 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
    13381 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
    13441 agcctcagac agtggttcaa agttt
//
