LOCUS       sequence-386454-        9089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhiC31 target with pUBQ10 switching from mTurq to mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: T1P/pUBQ10(mTurq->mScarlet)
FEATURES             Location/Qualifiers
     source          1..9089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    82..106
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      257..692
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(972..991)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             983..1798
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             983..1798
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1056..1075)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1899..2487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2388..2407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2554..2576
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(2600..2852)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2892..3686)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3003..3022)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3613..3632
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3720..3899)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     4131..4153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4145..4162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4146..4162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4172..4191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4172..4190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(4200..4841)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(4850..5542)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(5125..5144)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5394..5412
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(5558..5578)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    7681..7714
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             7738..7758
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7774..7776,7777..7779,7780..8493)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7819..7840)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8080..8099)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8427..8448
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aagtaaattg atgatcacaa gtgaaagtgt atgttgctgc cacagaatca ggggataacg
       61 caggaaagaa catgaaggcc ttgacaggat atattggcgg gtaaactaag tcgctgtatg
      121 tgtttgtttg agatctaatt ccggggatcg gaaatccaga agcccgagag gttgccgcct
      181 ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac gatctgttgc ggccggccgc
      241 cgggttgtgg gcaaaggcgc tcgacggtgg gcaaccgctt gcggttgtcc acgggcggag
      301 ccggtgcgcg tagcgcattg tccacaagcc aagggcgacc aataattgat atatatattc
      361 ataattgaaa agctaattga acatactact tgctgtaact acttgccgga gcgaggggtg
      421 tttgcaagct gttgatctga aagggctatt agcgttctca cgtgcctttt tgattagcga
      481 tttcacgtga ccttattagc gatttcacgt actccgatta gcgatttcac gtaccctgat
      541 tagcgatttc acgtggatag tttttggagc gggccggaaa gccccgtgaa tcaaggcttt
      601 gcggggcatt agcggtttca cgtggataac taccctctat ccacaggctt ccggggataa
      661 aaaagcccgc tcgacggcgg gctgttggat ggggatcgcc tgaatcgccc catcatccag
      721 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat
      781 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
      841 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtggctc aaaatctctg
      901 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      961 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcaaggcc
     1021 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1081 cgggcaatca ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt
     1141 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1201 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1261 tgcatggtta ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata
     1321 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1381 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     1441 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1501 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     1561 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     1621 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     1681 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     1741 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     1801 agaattggtt aattggttgt aaccatgacc aaaatccctt aacgtgagtt ttcgttccac
     1861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2521 gccttttgct ggccttttgc tcacatgaga tcttggcagg atatattgtg gtgtaacgtt
     2581 atcagcttgc atgccggtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     2641 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     2701 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     2761 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     2821 aagaaacttt attgccaaat gtttgaacga tctgcttgac tctagctaga gtccgaaccc
     2881 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     2941 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     3001 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     3061 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     3121 ccgtgggtca cgacgagatc ctcgccgtcg ggcatccgcg ccttgagcct ggcgaacagt
     3181 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     3241 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     3301 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     3361 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     3421 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     3481 cacgatagcc gcgctgcctc gtcttggagt tcattcaggg caccggacag gtcggtcttg
     3541 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3601 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3661 gcgtgcaatc catcttgttc aatcatgcct cgatcgagtt gagagtgaat atgagactct
     3721 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     3781 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     3841 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     3901 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     3961 ccttaattct ccgctcatga tcgataacat taacgcttac aatttccatt cgccattcag
     4021 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     4081 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4141 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcggagcgat
     4201 tgatgcatgt tgtcaatcaa ttggcaagtc ataaaatgca ttaaaaaata ttttcatact
     4261 caactacaaa tccatgagta taactataat tataaagcaa tgattagaat ctgacaagga
     4321 ttctggaaaa ttacataaag gaaagttcat aaatgtctaa aacacaagag gacatacttg
     4381 tattcagtaa catttgcagc ttttctaggt ctgaaaatat atttgttgcc tagtgaataa
     4441 gcataatggt acaactacaa gtgttttact cctcatatta acttcggtca ttagaggcca
     4501 cgatttgaca catttttact caaaacaaaa tgtttgcata tctcttatat tttcaaattc
     4561 aacacacaac aaataagaga aaaaacaaat aatattaatt tgagaatgaa caaaaggacc
     4621 atatcattca ttaactcttc tccatccatt tccatttcac agttcgatag cgaaaaccga
     4681 ataaaaaaca cagtaaatta caagcacaac aaatggtaca agaaaaacat gtttcccaat
     4741 gccataatac tcgaactcag taggattctg gtgtgtgcgc aatgaaactg atgcattgaa
     4801 cttgacgaac gttgtcgaaa ccgatgatac gaacgaaagc tcaagcttac ttgtacagct
     4861 cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc accacggtgt
     4921 agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg ccgggcatct
     4981 gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg ccgccgtcct
     5041 tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg tacaaccgct
     5101 cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca ggagggaagt
     5161 tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag gtgtcctggg
     5221 tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     5281 ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag gccctggagc
     5341 cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca cccttggtca
     5401 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     5461 cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg aactccttga
     5521 tcactgcctc gcccttgctc acaccactac cacttccgac tttcctcttc ttctttggca
     5581 ttgtttacta tagtagtgcc ccaactgggg taacctttga gttctctcag ttgggggcgt
     5641 agggctcaac aacaaacttt ccattcaccc atttatggat acacagtcta gctcaacaga
     5701 gcttttaacc caaattggta caatagaata caactttaga tcataattct caaaagaaag
     5761 agattcctta gctattctat ctgccactcc atttccttct cggcttgtat gcacaagcat
     5821 aaaatcctca aacttgctaa gtagatactt tatgtcttgg ataattggat tgagacttga
     5881 caagcataac tttcatgtaa ccaaagacac aagttgctga gaatccacct caaaaatgat
     5941 cttcctataa ttgaatcggg ataatgacag cacagcccat ctaagagcct ccacttctac
     6001 ttccagcacg cttcttactt ttaccacagc tcttgcacct aaccataaca ccttccctgt
     6061 atgatcgcga agcacccacc ctaagccaca ttttaatcct tctgttggcc atgccccatc
     6121 aaagttgcac ttaacccaag attgtggtgg agcttcccat gtttctcgtc tgtcccgacg
     6181 gtgttgtggt tggtgctttc cttacattct gagcctcttt ccttctaatc cactcatctg
     6241 catcttcttg tgtccttact aatacctcat tggttccaaa ttccctccct ttaagcacca
     6301 gctcgtttct gttcttccac agcctcccaa gtatccaagg gactaaagcc tccacattct
     6361 tcagatcagg atattcttgt ttaagatgtt gaactctatg gaggtttgta tgaactgatg
     6421 atctaggacc ggataagttc ccttcttcat agcgaactta ttcaaagaat gttttgtgta
     6481 tcattcttgt tacattgtta ttaatgaaaa aatattattg gtcattggac tgaacacgag
     6541 tgttaaatat ggaccaggcc ccaaataaga tccattgata tatgaattaa ataacaagaa
     6601 taaatcgagt caccaaacca cttgcctttt ttaacgagac ttgttcacca acttgataca
     6661 aaagtcatta tcctatgcaa atcaataatc atacaaaaat atccaataac actaaaaaat
     6721 taaaagaaat ggataatttc acaatatgtt atacgataaa gaagttactt ttccaagaaa
     6781 ttcactgatt ttataagccc acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa
     6841 agaaataaag cacgaagaat tctagaaaat acgaaatacg cttcaatgca gtgggaccca
     6901 cggttcaatt attgccaatt ttcagctcca ccgtatattt aaaaaataaa acgataatgc
     6961 taaaaaaata taaatcgtaa cgatcgttaa atctcaacgg ctggatctta tgacgaccgt
     7021 tagaaattgt ggttgtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca
     7081 cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg
     7141 caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat
     7201 tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt
     7261 cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc
     7321 tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt
     7381 tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc
     7441 tttggtttag attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat
     7501 atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga
     7561 gttttgtcga ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct
     7621 agtttgtgcg atcgaatttg tcgattaatc tgagtttttc tgattaacag aggtggagta
     7681 cgcgcccggg gagcccaagg gcacgccctg gcacccgcac cgcggaccta aacaatgcca
     7741 aagaagaaga ggaaagtcgg aagtggtagt ggtatggtga gcaagggcga ggagctgttc
     7801 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     7861 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     7921 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg
     7981 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     8041 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     8101 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     8161 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagcgac
     8221 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     8281 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     8341 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     8401 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     8461 atcactctcg gcatggacga gctgtacaag taataaatct cgtctctgtt atgcttaaga
     8521 agttcaatgt ttcgtttcat gtaaaacttt ggtggtttgt gttttggggc cttgtataat
     8581 ccctgatgaa taagtgttct actatgtttc cgttcctgtt atctctttct ttctaatgac
     8641 aagtcgaact tcttctttat catcgcttcg tttttattat ctgtgcttct tttgtttaat
     8701 acgcctgcaa agtgactcga ctctgtttag tgcagttctg cgaaacttgt aaatagtcca
     8761 attgttggcc tctagtaata gatgtagcga aagtgttgag ctgttgggtt ctaaggatgg
     8821 cttgaacatg ttaatctttt aggttctgag tatgatgaac attcgttgtt gctaagaaat
     8881 gcctgtaatg tcccacaaat gtagaaaatg gttcgtacct ttgtccaagc attgatatgt
     8941 ctgatgagag gaaactgcaa gatactgagc ttggtttaac gaaggagagg cagtttcttc
     9001 cttccaaagc atttcatttg acaatgcctt gatcatctta agtagagttt ctgttgtgga
     9061 aagtttgaaa ctttgaagaa acgactctc
//
