LOCUS       sequence-386489-        6129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman 
            ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB
  TITLE     Recombinant probes for visualizing endogenous synaptic proteins in 
            living neurons.
  JOURNAL   Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017.
  PUBMED    23791193
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.04"; journalName: "Neuron"; date: 
            "2013-06-19- 19"; volume: "78"; issue: "6"; pages: "971-85"
COMMENT     Sequence Label: pGW_PSD95.FingR-YFP
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          720..1546
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1840..1863
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(1885..1887,1888..2601)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1927..1948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2188..2207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2535..2556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2717..2851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2754..2773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2808..2827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2874..2890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2874..2890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2887..2909)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2898..2914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2922..2928,2929..2946,2947..2952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(3104..3121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3275..3863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3355..3374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4034..4825,4826..4894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4657..4676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5104..5122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5160..5182)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5282..5301
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5533..5555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5547..5564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5548..5564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgca tgctatactg
      781 tttttggctt ggggtctata cacccccgct tcctcatgtt ataggtgatg gtatagctta
      841 gcctataggt gtgggttatt gaccattatt gaccactccc ctattggtga cgatactttc
      901 cattactaat ccataacatg gctctttgcc acaactctct ttattggcta tatgccaata
      961 cactgtcctt cagagactga cacggactct gtatttttac aggatggggt ctcatttatt
     1021 atttacaaat tcacatatac aacaccaccg tccccagtgc ccgcagtttt tattaaacat
     1081 aacgtgggat ctccacgcga atctcgggta cgtgttccgg acatgggctc ttctccggta
     1141 gcggcggagc ttctacatcc gagccctgct cccatgcctc cagcgactca tggtcgctcg
     1201 gcagctcctt gctcctaaca gtggaggcca gacttaggca cagcacgatg cccaccacca
     1261 ccagtgtgcc gcacaaggcc gtggcggtag ggtatgtgtc tgaaaatgag ctcggggagc
     1321 gggcttgcac cgctgacgca tttggaagac ttaaggcagc ggcagaagaa gatgcaggca
     1381 gctgagttgt tgtgttctga taagagtcag aggtaactcc cgttgcggtg ctgttaacgg
     1441 tggagggcag tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc agacataata
     1501 gctgacagac taacagactg ttcctttcca tgggtctttt ctgcagtcac cgtccttgac
     1561 acgaagctta tgctcgaggt caaggaagca tcaccaacca gcatccagat cagctgggtg
     1621 ctccacttgc gccacgttcg ctactaccgc atcacctacg gtgaaactgg tggcaatagc
     1681 cctgtccagg aattcaccgt gcctggcagc aagtccactg ctaccatcag cggcctgaaa
     1741 cctggtgtcg actataccat cacggtgtac gccgtcacga tcttcagcgc ctaccgctcc
     1801 gcctggccgc cgatctccat caactaccgc accggatccg attacaagga tgacgacgat
     1861 aagggtagcg gctccagttc tagagtgagc aagggcgagg agctgttcac cggggtggtg
     1921 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1981 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     2041 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     2101 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2161 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2221 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2281 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2341 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     2401 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2461 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     2521 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2581 atggacgagc tgtacaagta ggaattcatt gatcataatc agccatacca catttgtaga
     2641 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2701 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2761 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2821 actcatcaat gtatcttatc atgtctggat ccgtaccgag ctcgcgtaat catgtcatag
     2881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tacattaatt gcgttgcgct
     3001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     3061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa taagcggccg
     4921 cggccatgcc ggccactagt ctcgagttat tattgaagca tttatcaggg ttattgtctc
     4981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5041 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5101 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5161 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5221 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5281 gcggcatcag agcagattgt actgagagtg caccatatga agacgtcgcc tcctcactac
     5341 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     5401 cagggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5461 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5521 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcccgt gtcaaggacg
     5581 gtgactgcag tgaataataa aatgtgtgtt tgtccgaaat acgcgttttg agatttctgt
     5641 cgccgactaa attcatgtcg cgcgatagtg gtgtttatcg ccgatagaga tggcgatatt
     5701 ggaaaaatcg atatttgaaa atatggcata ttgaaaatgt cgccgatgtg agtttctgtg
     5761 taactgatat cgccattttt ccaaaagtga tttttgggca tacgcgatat ctggcgatag
     5821 cgcttatatc gtttacgggg gatggcgata gacgactttg gtgacttggg cgattctgtg
     5881 tgtcgcaaat atcgcagttt cgatataggt gacagacgat atgaggctat atcgccgata
     5941 gaggcgacat caagctggca catggccaat gcatatcgat ctatacattg aatcaatatt
     6001 ggccattagc catattattc attggttata tagcataaat caatattggc tattggccat
     6061 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
     6121 cgccatgtt
//
