LOCUS       sequence-386615-        9165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9165)
  TITLE     Chen lab plasmids Dec 2022
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sgNS-Hygromycin
FEATURES             Location/Qualifiers
     source          1..9165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        126..505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        507..705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     660..680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             723..903
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    950..1075
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1568..1801
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1986..2030
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2328..2445
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2496..2744
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2496..2516
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2667..2686
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2769..2844
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2906..3117
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    4141..4729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4194..4214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4612..4623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4801..5034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5060..5077)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5066..5290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5336..5764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5423..5442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5633..5654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5773..5793)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5778..6107
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5958..6093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6020..6039
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6155..6202
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6221..6595
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6725..6846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6762..6781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6816..6835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6895..6911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6895..6911)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6908..6930)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6919..6935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6943..6949,6950..6967,6968..6973))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6988..7009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7126..7143)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7297..7885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7377..7396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8056..8847,8848..8916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8679..8698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8917..9021)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9096..9115)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
       61 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
      121 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      181 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      241 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      301 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      361 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      421 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      481 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      541 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      601 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      661 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
      721 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      901 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      961 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1021 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1081 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1141 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1201 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1261 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1321 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1381 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1441 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1501 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1561 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1621 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1681 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1741 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1801 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1861 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1921 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1981 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2041 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2101 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2161 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2221 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     2281 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     2341 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2401 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2461 gacagcagag atccagtttg gttaattaag gtaccgaggg cctatttccc atgattcctt
     2521 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     2581 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2641 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2701 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgtgcga atacgcccac
     2761 gcgatggggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     2821 gaaaaagtgg caccgagtcg gtgctttttt gaattcgcta gctaggtctt gaaaggagtg
     2881 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     2941 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
     3001 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     3061 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggac
     3121 cggttctaga gcgctgccac catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     3181 tttctgatcg aaaagttcga cagcgtgtcc gacctgatgc agctctcgga gggcgaagaa
     3241 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     3301 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     3361 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     3421 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     3481 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     3541 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     3601 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     3661 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     3721 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     3781 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     3841 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     3901 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     3961 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     4021 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     4081 gcggccgtct ggaccgatgg ctgtgtagaa gtacttcgcc gatagacgcg ttaagtcgac
     4141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4501 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4681 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     4741 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4801 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4861 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4921 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4981 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5041 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5101 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5161 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5221 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5281 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5521 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5641 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5761 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6121 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6181 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     6241 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     6301 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     6361 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     6421 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     6481 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     6541 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     6601 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6661 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6721 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6841 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6901 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6961 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7021 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7081 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7981 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8041 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8101 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8161 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8221 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8281 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8341 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8401 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8461 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8521 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8581 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8641 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8701 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8761 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8821 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8881 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8941 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9001 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9061 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9121 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgag
//
