LOCUS       sequence-386683-       12131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008."
COMMENT     Sequence Label: pHR-ON VIPER CAR (anti-CD19)-final design
FEATURES             Location/Qualifiers
     source          1..12131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1216..1281
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1411..1431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1856..1990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1893..1912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1947..1966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2158..2791
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2838..2963
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3460..3693
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3878..3922
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4207..4324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4398..5576
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4629..5567
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5524..5544
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5613..5622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6408..6449
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7596..7622
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8152..8740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8205..8225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8623..8634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8743..8759)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8744..8760)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8850..9083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9108..9126)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9109..9126)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9416..9435)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9649..9668)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9768..9790
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9828..9846)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9914..10018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10019..10087,10088..10879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10237..10256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11050..11638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11539..11558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11792..11809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11879..11899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11884..12131,1..82)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12064..12131,1..68)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
       61 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
      121 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
      181 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
      241 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
      301 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
      361 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
      421 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
      481 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
      541 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
      601 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
      661 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
      721 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
      781 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
      841 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
      901 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
      961 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     1021 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     1081 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     1141 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     1201 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     1261 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     1321 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     1381 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     1441 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     1501 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     1561 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     1621 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     1681 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     1741 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1801 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     1861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1981 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     2041 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     2101 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     2161 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     2221 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     2281 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     2341 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     2401 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     2461 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     2521 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     2581 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     2641 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     2701 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     2761 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     2821 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     2881 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     2941 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     3001 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     3061 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     3121 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     3181 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     3241 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     3301 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     3361 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     3421 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     3481 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     3541 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     3601 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     3661 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     3721 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3781 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3841 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3901 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3961 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     4021 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     4081 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     4141 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     4201 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     4261 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     4321 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     4381 attcctgcag cccgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     4441 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     4501 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     4561 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     4621 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     4681 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     4741 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     4801 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     4861 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     4921 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     4981 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     5041 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     5101 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     5161 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     5221 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     5281 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     5341 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     5401 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     5461 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     5521 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagggg
     5581 atctggagct ctcgagaatt ctcacgcgtg ccgccaccat ggccttacca gtgaccgcct
     5641 tgctcctgcc gctggccttg ctgctccacg ccgccaggcc ggacatccag atgacacaga
     5701 ctacatcctc cctgtctgcc tctctgggag acagagtcac catcagttgc agggcaagtc
     5761 aggacattag taaatattta aattggtatc agcagaaacc agatggaact gttaaactcc
     5821 tgatctacca tacatcaaga ttacactcag gagtcccatc aaggttcagt ggcagtgggt
     5881 ctggaacaga ttattctctc accattagca acctggagca agaagatatt gccacttact
     5941 tttgccaaca gggtaatacg cttccgtaca cgttcggagg cggcaccaag ctggagatca
     6001 caggtggcgg tggctcgggc ggtggtgggt cgggtggcgg cggatctgag gtgaaactgc
     6061 aggagtcagg acctggcctg gtggcgccct cacagagcct gtccgtcaca tgcactgtct
     6121 caggggtctc attacccgac tatggtgtaa gctggattcg ccagcctcca cgaaagggtc
     6181 tggagtggct gggagtaata tggggtagtg aaaccacata ctataattca gctctcaaat
     6241 ccagactgac catcatcaag gacaactcca agagccaagt tttcttaaaa atgaacagtc
     6301 tgcaaactga tgacacagcc atttactact gtgccaaaca ttattactac ggtggtagct
     6361 atgctatgga ctactggggc caaggaacct cagtcaccgt ctcctcaggg aagcctatcc
     6421 cgaaccctct gttgggtctc gatagtacca atggggccgc aaccacgacg ccagcgccgc
     6481 gaccaccaac accggcgccc accatcgcgt tgcagcccct gtccctgcgc ccagaggcgt
     6541 gccggccagc ggcggggggc gcagtgcaca cgagggggct ggacttcgcc tgtgattttt
     6601 gggtgctggt ggtggttggt ggagtcctgg cttgctatag cttgctagta acagtggcct
     6661 ttattatttt ctgggtgagg agtaagagga gcaggctcct gcacagtgac tacatgaaca
     6721 tgactccccg ccgccccggg cccacccgca agcattacca gccctatgcc ccaccacgcg
     6781 acttcgcagc ctatcgctcc gaggacgtgg tgtgctgcca ctcaatctac ggcaagaaga
     6841 agggtgatat cgacacctac cgatacatag gctcttccgg gacaggctgc gtggtcatag
     6901 tgggcaggat cgtcttgtcc ggatccggca ctagtgcgcc catcacggcg tacgcccagc
     6961 agacgagagg cctcctaggg tgtataatca ccagcctgac tggccgggac aaaaaccaag
     7021 tggagggtga ggtccagatc gtgtcaactg ctacccaaac cttcctggca acgtgcatca
     7081 atggggtatg ctgggcagtc taccacgggg ccggaacgag gaccatcgca tcacccaagg
     7141 gtcctgtcat ccagatgtat accaatgtgg accaagacct tgtgggctgg cccgctcctc
     7201 aaggttcccg ctcattgaca ccctgtacct gcggctcctc ggacctttac ctggtcacga
     7261 ggcacgccga tgtcattccc gtgcgccggc gaggtgatag caggggtagc ctgctttcgc
     7321 cccggcccat ttcctacttg aaaggctcct cggggggtcc gctgttgtgc cccgcgggac
     7381 acgccgtggg cctattcagg gccgcggtgt gcacccgtgg agtggctaaa gcggtggact
     7441 ttatccctgt ggagaaccta gagacaacca tgagatcccc ggtgttcacg gacaactcct
     7501 ctccaccagc agtcaccctg acgcacccaa tcaccaaaat cgatagggag gttctctacc
     7561 aggagttcga tgagatggaa gagtgctctc agcactatcc ctacgatgtg cccgattacg
     7621 ctaaacgggg cagaaagaaa ctcctgtata tattcaaaca accatttatg agaccagtac
     7681 aaactactca agaggaagat ggctgtagct gccgatttcc agaagaagaa gaaggaggat
     7741 gtgaactgag agtgaagttc agcaggagcg cagacgcccc cgcgtacaag cagggccaga
     7801 accagctcta taacgagctc aatctaggac gaagagagga gtacgatgtt ttggacaaga
     7861 gacgtggccg ggaccctgag atggggggaa agccgagaag gaagaaccct caggaaggcc
     7921 tgtacaatga actgcagaaa gataagatgg cggaggccta cagtgagatt gggatgaaag
     7981 gcgagcgccg gaggggcaag gggcacgatg gcctttacca gggtctcagt acagccacca
     8041 aggacaccta cgacgccctt cacatgcagg ccctgccccc tcgctaaact agtgcggccg
     8101 cgctctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct
     8161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8521 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8701 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     8761 gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     8821 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     8881 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8941 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9001 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9061 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     9121 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     9181 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     9241 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     9301 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     9361 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     9421 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     9481 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     9541 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     9601 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     9661 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     9721 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9781 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     9841 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     9901 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9961 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10021 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10081 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10141 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10201 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10261 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10321 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10381 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10441 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10501 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10561 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10621 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10681 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10741 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10801 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10861 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    10981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11161 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    11701 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    11761 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11821 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
    11881 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    11941 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    12001 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    12061 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    12121 ttttttattt a
//
