LOCUS       sequence-386787-        1545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable and dox. inducible expression of Trim69 Full length, isoform 
            A (codon optimized) after retroviral-mediated gene transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Song Y, Nguyen XN, Kumar A, da Silva C, Picard L, Etienne L, 
            Cimarelli A
  TITLE     Trim69 is a microtubule regulator that acts as a pantropic viral 
            inhibitor.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 
            10.1073/pnas.2211467119. Epub 2022 Oct 17.
  PUBMED    36251989
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2211467119. 
            doi"; pages: " 10.1073/pnas.2211467119. Epub 2022 Oct 17"
COMMENT     Sequence Label: pRetroX-Tight-Pur-Trim69 Full length, isoform ...
FEATURES             Location/Qualifiers
     source          1..1545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgataagggt ggcggagcgg ccgccgaggt gtccaccaac
       61 cccagctcca acattgaccc tggcgactac gtggagatga acgatagcat cacccacctg
      121 ccctccaagg tggtcatcca ggacatcacc atggagctgc actgccctct gtgtaacgac
      181 tggttcagag accctctgat gctgtcctgt ggccacaact tctgcgaggc ctgcatccaa
      241 gacttttgga ggctgcaggc caaggagacc ttttgccccg agtgcaagat gctgtgccag
      301 tacaacaact gcacattcaa ccccgtcctg gataagctgg tggagaaaat caagaagctg
      361 cccctcctga aaggccaccc ccagtgtcct gagcacggcg aaaacctcaa actgttctcc
      421 aagcccgacg gcaaactgat ttgtttccag tgcaaggatg ccaggctgag cgtgggccag
      481 tccaaggagt ttctgcaaat ctccgacgcc gtgcacttct tcaccgagga gctggccatt
      541 cagcagggcc agctggagac caccctgaag gagctgcaaa ccctcaggaa catgcagaag
      601 gaggccatcg ccgctcacaa ggaaaacaag ctgcacctcc agcagcacgt gtccatggag
      661 ttcctcaagc tccatcagtt cctgcactcc aaggaaaagg atatcctcac cgagctgaga
      721 gaggaaggca aagccctgaa tgaagagatg gagctgaacc tgagccagct ccaggagcag
      781 tgcctgctgg ccaaggacat gctcgtgagc atccaggcca agaccgaaca gcagaacagc
      841 ttcgacttcc tcaaggatat taccaccctg ctgcactccc tggagcaggg catgaaggtc
      901 ctggccacca gagagctcat ttccaggaag ctgaacctgg gccagtataa gggccccatt
      961 cagtacatgg tgtggaggga gatgcaggac accctgtgtc ccggcctgag ccctctgaca
     1021 ctggacccca aaacagccca ccccaatctg gtgctgtcca agagccagac ctccgtgtgg
     1081 catggcgaca tcaagaagat catgcccgat gaccccgaga ggtttgacag cagcgtcgct
     1141 gtgctgggct ccagaggctt taccagcggc aaatggtact gggaagtgga ggtggccaaa
     1201 aagaccaaat ggacagtggg agtggtgagg gaaagcatta tcaggaaggg cagctgtcct
     1261 ctgacacctg agcagggctt ttggctgctg aggctgagaa accagacaga cctgaaggcc
     1321 ctggacctcc ccagcttcag cctgaccctg acaaacaacc tggacaaggt cggcatctac
     1381 ctggactacg agggaggcca gctgagcttc tataatgcca aaaccatgac acacatctat
     1441 accttttcca acacatttat ggagaagctg tacccttatt tctgtccctg cctgaacgac
     1501 ggcggcgaaa ataaggagcc cctgcacatt ctgcaccccc agtga
//
