LOCUS       sequence-386836-       12511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  zebrafish expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12511)
  TITLE     Koster Lab Plasmids 2022
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTol2-Synapse reporters
FEATURES             Location/Qualifiers
     source          1..12511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(30..41,42..179)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    365..459
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    609..703
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             1569..1622
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1623..2000
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      2013..2022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5228..5237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6264..6358
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6522..6616
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      9136..9145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9871..10005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9895..9914)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9949..9968
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(10108..10126)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(10133..10320,10321..10332)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10362..10378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10362..10378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10375..10397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10386..10402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10410..10416,10417..10434,10435..10440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10455..10476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10593..10610)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10764..11352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10844..10863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11523..12314,12315..12383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12146..12165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12384..12488)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaatc ccatatgggc agaggtgtaa aaagtactca aaaattttac
       61 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
      121 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
      181 gatctgtaat acgactcact atagggacat agaatgcagg tacgtaatac gactcactat
      241 agttctagcc tcgagactag tcctaggcca tcatcgataa gcttgatatc agtttaacgt
      301 gtggaggagc tcaaagtgag gctgagacgc gatgggatcc attatatacc ctctagagtc
      361 tccgctcgga ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc
      421 gctcggagga cagtactccg ctcggaggac agtactccga cctgcagccc gggggatcgt
      481 cgacaggtcc tagctagggc tgctctccaa tctctgtcat ggcagggcca ctggcatccc
      541 tgaagttggt ggagaccacc tcactccact cagcatacag ctcatccagt cctcttaggg
      601 ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
      661 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac tctagagggt
      721 atataatgga tcccatcgcg tctcagcctc actttgagct cctccacacg aattcgatat
      781 ccaccatggg ctgtgtgcaa tgtaaggata aagaagcaac aaaactgacg tcgacgatgg
      841 tgagcaaggg cgaggagctg ttcacaggag tggtgcctat cctggtggag ctggacggag
      901 acgtgaacgg acacaagttc agcgtgagag gagagggaga gggagacgct acaaacggaa
      961 agctgacact gaagttcatc tgtacaacag gaaagctgcc tgtgccttgg cctacactgg
     1021 tgacaacatt cggatacgga gtggcttgtt tcagcagata ccctgaccac atgaagcagc
     1081 acgacttctt caagtctgct atgcctgagg gatacgtgca ggagagaaca atcagcttca
     1141 aggacgacgg aacatacaag acaagagctg aggtgaagtt cgagggagac acactggtga
     1201 acagaatcga gctgaaggga atcgacttca aggaggacgg aaacatcctg ggacacaagc
     1261 tggagtacaa cttcaacagc cactatgtgt acatcacagc tgacaagcag aagaactgta
     1321 tcaaggctaa cttcaagatc agacacaacg tggaggacgg aagcgtgcag ctggctgacc
     1381 actaccagca gaacacacct atcggagacg gacctgtgct gctgcctgac aaccactacc
     1441 tgagccacca gagcaagctg tctaaagacc ctaacgagaa gagagaccac atggtgctgc
     1501 tggagttcgt gacagctgct ggaatcacac atggcatgga cgagctgtac aagactagtg
     1561 gatccggcga gggaagagga agtctgctga cctgcggaga cgtggaggag aaccctggac
     1621 ctatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga
     1681 ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag agctattcca
     1741 tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcta
     1801 tgggcatcat gaactcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc
     1861 gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc
     1921 gcctgctgct gcctggggag ttggccaagc atgccgtgtc cgagggtact aaggccatca
     1981 ccaagtacac cagcgctaag gacccaccgg tcgccaccat ggtgtcaaaa ggagaggaac
     2041 tgtttactgg agtcgtgcct atcctggtgg agctggatgg agatgtgaat ggacacaagt
     2101 tctctgtgtc tggagaggga gagggagatg ctacatatgg aaagctgaca ctgaagttca
     2161 tctgtacaac aggaaagctg cctgtgcctt ggcctacact ggtgacaaca ctgtcttggg
     2221 gagtgcagtg ttttgctaga taccctgacc acatgaagca gcatgacttc ttcaagtctg
     2281 ctatgcctga gggatatgtg caggagagaa caatcttctt caaggatgat ggaaactaca
     2341 agacaagagc tgaggtgaag tttgagggag acacactggt gaacagaatt gagctgaagg
     2401 gaattgactt caaggaggat ggaaacatcc tgggacacaa gctggagtac aattatttct
     2461 ctgacaatgt ctacatcaca gctgacaagc agaagaatgg aatcaaggct aacttcaaga
     2521 tcagacacaa cattgaggat ggaggagtgc agctggctga ccactaccag cagaacacac
     2581 ctattggaga tggacctgtg ctgctgcctg acaaccacta cctgagcaca cagtctaagc
     2641 tgagcaagga ccctaatgag aagagagacc acatggtgct gctggagttt gtgacagctg
     2701 ctggaatcac actgggcatg gacgagctgt acaagtaaac tcgagcctct agaactatag
     2761 tgagtcgtat tacctagtaa ttaagtctca gccaccgtta actgaacatg tcaaaacctg
     2821 tggagactgt tgagatttga tgttctgaaa agataaagcc tataaataaa atgttgccca
     2881 aatttcctgc ctgatgtttt tctttgtctt tgctacatgg ctttgctgct cggatcggct
     2941 cactctgtgt atgccacgtt cactttgtac tctccttctc acggtaggtt tattattttt
     3001 agatgtgcag ttagtttctg tgaaataaca caccacacac tgatattgtc tgtgcattga
     3061 cttggtgagt gcacattgtt tttgatcttg acatatttat atttgattga tcaggtgaac
     3121 tgtgtgaatc taaagtgctc catacagatg ttctgcattg aaaatattct cattttatta
     3181 gtggaagtga gtgtatgcca gcgcaggtac cgggcccaat gcattggcgc cgcggccggc
     3241 cgctaccacg caccaccatg aagagtttta cagaaacgac tataaagatg ttaaattcaa
     3301 aaccatttgt tttattccgc ggccggccgc cctttttttt tttttttttt tttttttttt
     3361 ttttttaacg aaattgtttt catttttatt ttggttttct ccagaggctc agtttccttc
     3421 cacacaggga caatcaagga acatcaaaac ttttgaaata tataatcaac aatataacac
     3481 ttaaaacgat gggctcagaa ataagacata acagagtcca tgaattaaaa attgcaccaa
     3541 caatccattc aacatgcttg tctttctatt tgcctaatct aaaatacttc cctttcctgt
     3601 attgcccttg ccatccattg atttatgtcc tcctgtagac gttggttggc gtccaagtga
     3661 ggccaagatt caataaccct gtcattccat cagtcctttt ccataaccac ttccacgaca
     3721 tgcctatctt ttccaaacat ttcttgcaat actgcaatca gacccctgga ggtcacattg
     3781 aagtgccatt atttcacaga attgccctgt tccagtgcaa tagtaacaaa agaaatcaca
     3841 caaataaaac acgacaaatt tcactgaagt tacttgtaga taaaagaagc aaacgttcaa
     3901 ataaaactta aatgagatgt tacagtcctc ccaatttgta acactgattc tacctggatg
     3961 tttgccactt ttcgcttccc tgttctgtac tgcaccatca gaatggtaaa ctaatacact
     4021 catacacact aaaaagagtg aactgtaatc gtaaacactg ttacccactc taatccatgt
     4081 ttaaaaaaca ttctttctgc cttcctctgg tcatttttgg cccctttttg tctagtgcta
     4141 atttctactt catctgattg agacactttc ataaaagttt tgctacattt actcaaaact
     4201 taaacacact accacctccc caaaacaccc tttagtttcc ttcgaattgt tgttgttgta
     4261 atacgactca ctatagttct agaggctcga gactagattt agctctccag agctgtgatt
     4321 cttgtagcga ttctctcagc cagtctcttg cagatagctc tcagctcgaa tctgatgaat
     4381 ctaggcaggt agtggtggca caccaccagt ccccacagct ttcctcccac caccaggctc
     4441 acagccagtg tagctctcac tcccatgtcc ttcaggaact gcaggtggca agggctcatg
     4501 cttctcagga agcatccgct catgtccagg tctcttcctg tcagagggct cagtctaggc
     4561 tccagaggca caggctggta tgtcacgtcc accagcactc tcactctctg tctcacgtac
     4621 agctgtctag ccatctgagg cactgtgctg ctagggtatc tgtttccgaa gtagctctcc
     4681 agtccaggca cgtggcactc gctgaacacc agtccgtgtc cctgctcgtc gaatctatac
     4741 accatcactc tgtcgtatcc tgtgcactgc tggaacagca gcactgtgtc gtcgcacaga
     4801 gctctcaggc ttccagctgt tctgattctc tccagagcag gagccagtgt tccgctcagg
     4861 tcgatgctag gtccagctct ctccagctcg atgatcagtc ctccctcagg aggtctgtgc
     4921 atcagtccgc agtactctgt gctagggttt ccgattctgc atctcacagc cacaggcatt
     4981 ccctcagctg tagggtccag gtgaggcagg atcttgatca gcaggtctcc gtcgatctca
     5041 gccagaggca ctcccagcac gcttcccagg ttcaggaact cagcagcgtt agcagaagcc
     5101 tggatcactc tgtggtcgtg ctcgctcacc accagcagag ctccgtgagg ctggatgctt
     5161 ccagccaggt ggatctcctc gtgctcgcat gtcagcaggt caggctgtct agccacgctt
     5221 ccctcagcca tggtggcgac cggccggtgg atcccgggtc ctcctccaca catctcgttg
     5281 gagaaggatg tgggctctcc ctgaccatac actccttgat tgtatccacc gccttcatat
     5341 ccaccctgct ggccgtagtc aggctgatat ccaccctgag agctggcata ctggtcctgt
     5401 ccataacctt cctgtccata gccctggccg aaggagtctg gagctggctg cttctctcca
     5461 gatggcatgt aggtgccagc gaaagccgcc atccagccgg tttccttgaa gatgaaccag
     5521 aggttgcctc cccataggat cacattacag aacccgaatg ccacagatgt gttcaggcca
     5581 gacaccaccg gttcgtgcag ctctttgcat cggttttcct ctcgttcgca cgcagggatg
     5641 aggttgataa cgtcatcagg gtcggtggct ctcttcacgt ctgagagacc tttagcccac
     5701 gccgatgaac tcaccagcca catgaaggtg aaaaccgctg tcacaatgaa atcagcctga
     5761 gctcctttgt ttccctcgcg gtatttctcc aacaggaaga tgtacacaga aatggctgcc
     5821 attgaataaa ggaaggagaa gacgccgatg gtgacgaaga actctgctga ggaagagttg
     5881 tctcccacca gaaagagtcg ctcgggttct gatcctttac acgtaggggc atcaaaccat
     5941 acctggtgca gtctgaacgg gtacgcaaac tcaacctcaa tgttcaggtc actctctgag
     6001 cggttctcac actccacgct catacgaaac actccagagt aaccaccgca ggtggcgaac
     6061 gcgaagatgg caaagaacca ttgtaagatt ttgatgaatc ctaatggctg tttgacgatt
     6121 gtatgcccag tggcgaccaa ctggttggca acatccatgg tggatatcga attatcgttt
     6181 aaacagatca gtttaacgtg tggaggagct caaagtgagg ctgagacgcg atgggatcca
     6241 ttatataccc tctagagtct ccgctcggag gacagtactc cgctcggagg acagtactcc
     6301 gctcggagga cagtactccg ctcggaggac agtactccgc tcggaggaca gtactccgac
     6361 ctgcagcccg ggggatcgtc gacaggtcct agctagggct gctctccaat ctctgtcatg
     6421 gcagggccac tggcatccct gaagttggtg gagaccacct cactccactc agcatacagc
     6481 tcatccagtc ctcttaatta agtaccagct gacctgcagg tcggagtact gtcctccgag
     6541 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     6601 agtactgtcc tccgagcgga gactctagag ggtatataat ggatcccatc gcgtctcagc
     6661 ctcactttga gctcctccac acgaattcag atatcagcca tcatgcctct caaacgagaa
     6721 gatacggagc gtgcgctgca ggccatggaa gcctgtcagt ctgcaggtga tgagggtttc
     6781 aggactcgcg cagagagact tctgaccatc tttcagagtg acctgtttca ggctctgctg
     6841 gacatccagg agttctatga gctgacggtg tttgagaatc agacggcagg tagagctctc
     6901 acaccgggac tgaagtaccg ttaccatgac gaggagacgc cgccactgca gcacagtccc
     6961 gctcacctga gcacaggtaa atctgcagag atgctccacc tgggtgactc tggccacgcc
     7021 cccatcgacg gcatccacgc atacacacct cagatgcacg tgtcacctgc aaagccagtg
     7081 ttactcccga gtggacacgc tccctactac gcaacctcta ctctgatgaa cggtatggac
     7141 ggagatgttg aatatgagga gatcacgctg gagaggggga actctgggct gggcttcagt
     7201 atcgccggag ggactgataa ccctcatata ggagacgatc ccagtatctt catcactaag
     7261 atcataccag gaggagctgc ggcacaggac ggacgactca gagtgaacga cagcattctg
     7321 ttcgtcaatg acgtggatgt gcgggaagtc acacacagct ttgcggtgga ggcgctgaag
     7381 gaagcaggac cgatcgtccg gctgtacgtg ctgcgccaca aaccatcagc agagaaaatc
     7441 actgagctca agctcatcaa aggccctaaa ggtctgggct tcagtattgc gggtggtgtg
     7501 gggaaccagc acgtgcccgg agacaacagc atctacgtta ccaagatcat cgagggcgga
     7561 gctgcacata aagacggacg gctgcagatc ggagacaaga tcctcgctgt gaacaacatg
     7621 tatctggagg aggtgatgca tgaagacgct gtagcggcgc tgaagaacac tggagacgtg
     7681 gtgtttctga gagtcgccaa aacactccat cagcatcatc atcaggacgc ctacaaccct
     7741 cctgacatca ccagctcata ttctcctcat atggacatgt ctgattaccc gcaagccctc
     7801 agtccctcgt ctccgcgccg ttactccccc atccctaaag gcctgtttct ggacgacgac
     7861 atttccaggg agccgcggcg tgtggtgatc cacagaggct ctacaggtct gggctttaat
     7921 atcgtgggtg gagaagatgg agagggaatc ttcatctcct tcatactggc tggaggagct
     7981 gctgatctga gcggagagct gaggaaagga gaccagatac tgagtgtgaa cggtgtagat
     8041 ctgcgtcacg ccactcatga acaggccgcg gcggcactga agaacgcagg acagacggtc
     8101 accatcatca cacagtacag acctgaagaa tacagcaggt tcgaggccaa gatccatgac
     8161 ctgcgggagc agctgatgaa cagcagttta gtgtctgcag cggcttcatt acggagcgga
     8221 aagagaagct tcttcatcag ggctctgttt gactatgata agacggcaga cggtggtttt
     8281 ctgtcgcagg ccgtgagctt caggttcggg gatgttctgc aggtctttga ctgcagtgat
     8341 gaggaatggt ggcaggccgg aaaactggct ccacacggag aactggagga gaccgggtac
     8401 attcccagca agaggagggt ggagcggaag gaatggtctc gtctgaagac tcgaggccga
     8461 gagcccgtca gcggacgcag cgattacatc gtgagccatg agacggtgac gcagagtgaa
     8521 gtgcactacg cgcgtcccgt cataatcctc ggacccagca aggaccgtgt gaacgatgac
     8581 ctcctctccg agttcccgga caagtttggc tcctgtgtgc cccacacgac gcggccgaag
     8641 cgcgagtacg agatggacgg acgcgattac cactttgtgt cgtctcgaga gcagatggag
     8701 aaggacattc agagccatcg cttcatcgag gcgggacagt acaacagtca cctgtacggc
     8761 accagcgtcc agagcgtcag acaggtggca gagcagcaag ggaagcactg tatcctggat
     8821 gtttcagcga acgcagtgcg gaggctacag gccgctcagc tttatcccat cgccatcttt
     8881 atacgaccct catcactgca gaacgtacta aacattagca agcgtctgac ggaggaacag
     8941 gccagaagag cgttagacag agccgtcaaa ctggagcagg acttcatcga gtgtttctca
     9001 gcgatcgtgg agggcgagag cttcgaggag atctaccatc atgtgaagtc tgtgatcgag
     9061 gagcagtccg gaccgtatag ctggatccca gcacgagaga gactgggagg aggacccggc
     9121 atccaccggc cggtcgccac catggtgagc aagggcgagg cagtgatcaa ggagttcatg
     9181 agattcaagg tgcacatgga gggaagcatg aacggccacg agttcgagat cgagggagag
     9241 ggagagggaa gaccttacga gggaacacag acagctaagc tgaaggtgac aaagggagga
     9301 ccactgcctt tctcttggga catcctgagc cctcagttca tgtacggaag cagagctttc
     9361 atcaagcacc ctgctgacat ccctgactac tacaagcaga gcttccctga gggattcaag
     9421 tgggagagag tgatgaactt cgaggacgga ggagctgtga cagtgacaca ggacacaagc
     9481 ctggaggacg gaacactgat ctacaaggtg aagctgagag gaacaaactt ccctcctgac
     9541 ggacctgtga tgcagaagaa gacaatggga tgggaggctt ctacagagag actgtaccct
     9601 gaggacggag tgctgaaggg agacatcaag atggctctga gactgaagga cggaggaaga
     9661 tacctggctg acttcaagac aacatacaag gctaagaagc ctgtgcagat gcctggagct
     9721 tacaacgtgg acagaaagct ggacatcaca agccacaacg aggactacac agtggtggag
     9781 cagtacgaga gaagcgaggg aagacacagc accggcggca tggacgagct gtacaagtaa
     9841 atctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     9901 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     9961 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
    10021 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcc
    10081 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgggc ccaagtgatc
    10141 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
    10201 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
    10261 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
    10321 tttacacctc tgggtactgt ttcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    10381 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10501 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10561 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10621 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10681 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10741 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10801 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10861 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10921 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10981 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11041 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11101 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11161 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11221 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11281 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11341 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11401 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11461 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11521 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11581 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11641 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11701 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11761 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11821 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11881 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11941 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    12001 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    12061 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    12121 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12481 gttccgcgca catttccccg aaaagtgcca c
//
