LOCUS       sequence-387311-        2919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Cai YM, Witham S, Patron NJ
  TITLE     Tuning Plant Promoters Using a Simple Split Luciferase Method to 
            Assess Transcription Factor-DNA Interactions.
  JOURNAL   ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 
            10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
  PUBMED    37856867
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00094"; journalName: "ACS Synth Biol"; date: 
            "2023-11-17- 17"; volume: "12"; issue: "11"; pages: "3482-3486"
COMMENT     Sequence Label: pEPOZ0CM0137
FEATURES             Location/Qualifiers
     source          1..2919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      172..760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     661..680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     914..931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(955..1012)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      1907..1950
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1954..1972)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2134..2793
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2200..2219)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2814..2908
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
       61 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      121 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      181 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      241 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      301 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      361 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      421 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      481 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      541 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      601 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      661 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      721 atttttgtga tgctcgtcag gggggcggag cctgtggaaa aacgccagca acgcggcctt
      781 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      841 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      901 aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt
      961 cggttttaaa gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcaggg
     1021 tctcattcgg gcgggagcgg tggaagcggc gaaattcagc aagccactgc aggagtctca
     1081 caagacactt cggaaaatcc taacaaaaca atagttcctg cagcattacc acagctcacc
     1141 cctaccttgg tgtcactgct ggaggtgatt gaacccgagg tgttgtatgc aggatatgat
     1201 agctctgttc cagattcagc atggagaatt atgaccacac tcaacatgtt aggtgggcgt
     1261 caagtgattg cagcagtgaa atgggcaaag gcgatactag gcttgagaaa cttacacctc
     1321 gatgaccaaa tgaccctgct acagtactca tggatgtttc tcatggcatt tgccttgggt
     1381 tggagatcat acagacaatc aagcggaaac ctgctctgct ttgctcctga tctgattatt
     1441 aatgagcaga gaatgtctct accctgcatg tatgaccaat gtaaacacat gctgtttgtc
     1501 tcctctgaat tacaaagatt gcaggtatcc tatgaagagt atctctgtat gaaaacctta
     1561 ctgcttctct cctcagttcc taaggaaggt ctgaagagtc aagagttatt tgatgagatt
     1621 cgaatgactt atatcaaaga gctaggaaaa gccatcgtca aaagggaagg gaactccagt
     1681 cagaactggc aacggtttta ccaactgaca aagcttctgg actccatgca tgaggtggtt
     1741 gagaatctcc ttacctactg cttccagaca tttttggata agaccatgag tattgaattc
     1801 ccagagatgt tagctgaaat catcactaat cagataccaa aatattcaaa tggaaatatc
     1861 aaaaagcttc tgtttcatca aaaatgagct tcgagaccga attccagaaa tcatccttag
     1921 cgaaagctaa ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg
     1981 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac
     2041 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     2101 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     2161 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     2221 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     2281 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2341 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2401 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2461 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2521 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     2581 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     2641 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     2701 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2761 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
     2821 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     2881 ccgccgggcg ttttttattg gtgagaatcc aagcctcga
//
