LOCUS       sequence-38785-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0028
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             178..195
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg
      181 gtgccgcgcg gcagccatat ggcctctgga gccgattcaa aaggtgatga tttatcaaca
      241 gccattctca aacagaagaa ccgacccaat cggttaattg ttgatgaagc catcaatgaa
      301 gataacagcg tggtgtcctt gtcccagccc aagatggatg aactgcagtt gttccgaggt
      361 gacacggtgt tgctaaaagg aaagaaaaga cgggaagctg tatgcattgt tctttctgat
      421 gacacgtgtt ctgatgagaa gattcgaatg aatagagttg ttcggaataa cctccgagtt
      481 cgcctaggag atgtcatcag catccagcca tgccctgatg taaagtatgg caaacgtatc
      541 cacgttctac ccatcgatga cacagtggaa ggcatcactg gcaatctctt tgaggtatac
      601 cttaagccgt acttcctgga agcttatcgg cccatccgta aaggagatat ttttcttgtc
      661 cggggtggga tgcgtgctgt ggagttcaaa gttgtagaga cagatcccag cccttactgt
      721 attgttgctc cagacacagt gatccactgt gagggggagc caatcaaacg agaggatgag
      781 gaggaatcct tgaatgaagt aggctatgat gacgtcggtg gttgcaggaa gcagctagct
      841 cagataaagg agatggtgga gc
//
