LOCUS       sequence-388047-        6226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses tdTomato under a human choline acetyltransferase (hChAT) 
            promoter fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Santoscoy MC, Espinoza P, De La Cruz D, Mahamdeh M, Starr JR, Patel 
            N, Maguire CA
  TITLE     An AAV capsid increases transduction of striatum and a ChAT promoter
            allows selective cholinergic neuron transduction.
  JOURNAL   Mol Ther Methods Clin Dev. 2023 May 9;29:532-540. doi: 
            10.1016/j.omtm.2023.05.001. eCollection 2023 Jun 8.
  PUBMED    37359416
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2023-05-9"; volume: "29:532-540. doi"; 
            pages: " 10.1016/j.omtm.2023.05.001. eCollection 2023 Jun 8"
COMMENT     Sequence Label: pAAV-hChATp-tdTomato
FEATURES             Location/Qualifiers
     source          1..6226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR
     regulatory      1210..1219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2671..3259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2724..2744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3142..3153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3302..3423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3313..3332)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3367..3386
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3585..3714
                     /label=AAV2 ITR
     rep_origin      complement(3951..4539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4031..4050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4710..5501,5502..5570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5333..5352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5571..5675)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5701..6156)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5838..5859)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6050..6069
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      301 aagtgtatca cccgggagca gggggtgggg aaggaggagc agtggctggt ggcaagtctg
      361 agccaggaca tagccgatga cagcctgtga gtgaccggcc agactggggt tggaccttcg
      421 gggcagagca ggcagccctt ggaaagccag gctggtagga gcatgggtgg gaggcacaaa
      481 acacagacaa acagacatat atacacatat atcctctagg catctccgag gagtgatgga
      541 ggagagagtt ggtaggtggg accacaggaa accatcccac ccagctctgc cttcttcaag
      601 ctcagggtcc ctgaagcctg actagtgtct cccgaatggg ggaggggggg gctctgggca
      661 gagcaaaagg aggcattggc tgcatgtctt agggccttta ccaactgacc ttgtccttgc
      721 tgtcctctca aaagactgcc atgctccggt gaatcctcac caggacgcca cctctttcac
      781 tttgcagttg ggggcagccc ctacgaaccc acagcactgg gaggcgtggg aacaagcctg
      841 tggagagtgt ccctgcactg tgccagcaag ggctttacat tctctgccca gcactgcaag
      901 gcagccccag gctcctccat ccttgggcct ggttcatcca tctttctgga ctctctgttc
      961 ctggcaacaa ccctgccagt cccgatacat gggcggtgct tgcctctggg taggggtggt
     1021 gttgggtggg gagaggggtg aggaaattag ctaaacctca gctttagact tgagagaata
     1081 gagggtgggc tggtggaaaa catgagtatg tgcctgtgga ggcttaggtt cagggggatc
     1141 tcagagggta gcaattgtga cccacagcct aataataaca gtctttgccc tcttggcctg
     1201 gctcgtaccg ccaccatggt gagtaagggc gaggaagtga tcaaagagtt catgcggttt
     1261 aaggtgagaa tggaaggaag catgaacggc cacgagttcg aaattgaggg agaaggagag
     1321 ggacggccct acgagggcac ccagacagcc aagctgaaag tgacaaaggg cgggcctctg
     1381 ccattcgctt gggacatcct gagcccacag tttatgtacg gctccaaggc ctatgtgaaa
     1441 catccagctg acattcccga ttataagaaa ctgagcttcc ccgaggggtt taagtgggaa
     1501 agagtgatga acttcgagga cggaggcctg gtgactgtga cccaggacag ctccctgcag
     1561 gatgggaccc tgatctacaa ggtgaaaatg agagggacaa attttccccc tgatggacct
     1621 gtgatgcaga agaaaactat gggatgggag gcctccaccg aaaggctgta tccacgcgac
     1681 ggggtgctga aaggagaaat ccaccaggct ctgaagctga aagatggggg acattacctg
     1741 gtggagttca agacaatcta catggccaag aaacctgtgc agctgccagg ctactattac
     1801 gtggacacaa aactggatat cacttcacac aacgaggact acactattgt ggagcagtat
     1861 gaacggagcg aggggagaca ccatctgttc ctgggccatg ggactggaag taccggctca
     1921 gggtctagtg gaaccgcctc aagcgaggat aacaatatgg ctgtgatcaa agagttcatg
     1981 aggtttaagg tgcgcatgga gggcagcatg aatgggcacg aatttgagat tgaaggagag
     2041 ggcgaaggga ggccttacga gggcacacag actgccaagc tgaaagtgac caagggagga
     2101 ccactgcctt tcgcttggga tatcctgtct cctcagttta tgtacggaag taaggcctat
     2161 gtcaagcatc ccgctgacat tcctgattac aagaaactgt ctttcccaga gggctttaag
     2221 tgggagagag tgatgaattt tgaagatgga ggcctggtga ccgtgacaca ggactcctct
     2281 ctgcaggatg gcactctgat ctacaaagtc aaaatgcgcg gcaccaattt tccacccgat
     2341 gggcccgtga tgcagaagaa aacaatgggg tgggaggcca gcactgaacg gctgtatcct
     2401 agagacggag tgctgaaggg cgaaatccac caggccctga agctgaaaga cggcggccac
     2461 tacctggtgg agttcaaaac catctacatg gccaagaaac cagtgcagct gcccggctat
     2521 tactatgtgg acaccaagct ggatatcaca tcccacaatg aagactacac cattgtggaa
     2581 cagtatgaga ggtctgaagg acgccaccat ctgtttctgt acggcatgga tgagctgtat
     2641 aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     2701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3061 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3241 ctttgggccg cctccccgca tcgataccgt cgacccgggc ggccgcttcg agcagacatg
     3301 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3361 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3421 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     3481 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca
     3541 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat
     3601 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     3661 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat
     3721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5701 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5761 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5821 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5881 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5941 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     6001 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     6061 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     6121 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     6181 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgcca
//
