LOCUS       sequence-388094-        6909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the protein of wmCardinal in neurons of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pSF11-wmCardinal
FEATURES             Location/Qualifiers
     source          1..6909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4479..4501)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4601..4620
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4702..4720)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4788..4892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4893..4961,4962..5753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5111..5130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5924..6512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6413..6432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6666..6683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6800..6821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6836..6841,6842..6859,6860..6866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6874..6890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6879..6901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(6898..6909,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6898..6909,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa agtgaggggg ctcactgccc ccccccctct tctccttgaa gctcatccag
      241 acgtcgctgt cctcctcctc ctcgtccccg gggcgctgtg tgtgtgtgtg tgtgctccgt
      301 gagcatgtag cagcttcagc ccatttaaag gcgcacactc tctgcatctc tgcgtgtctc
      361 gacactcgtt ttttctcttc ttttctgctg cacacacaca cacacacaca cacacgctca
      421 tgccagagca cttcttgctc cggggggctc cgcgcggagc ccgagaggac ctcctcctcc
      481 tttttcttct tcgcttttta gttcttcttg cctcggtctc gccccttgtt ttgctctcca
      541 tgtagtatat acatctgggt gtgtgtgtgt gtgtgcacct ttggagcatt ttttgaacct
      601 ttccagaact ttggagcaac attttcagct tgttcagtgc actttttagg ctgaaaatcc
      661 tatttttagg ttttcgaaag tttttttgga gcatttttcg tgcaatttag gtgcaaaatt
      721 aaaaaaaagg gttttgtagt gaattaaaat ttttttgaaa ctttcgcctt tttaaaaaaa
      781 tgtttctaat atctttaaaa attggaatat tttggaaaac aatttgtttt caattggcaa
      841 tttattaatt ctgagcttct gagcgtaaat ttttttttat tttggagcaa ttctgagatg
      901 tcttagtgca tttttcgggc atacataaaa aaaaatttat cttaataata aaaaccttaa
      961 atttaggctt caccgtctgg aaattcaatt ttttcagctg aaaaactttc aaaaaaaaaa
     1021 aaattaaatt ttcacattta ttttctctgg aattttggag caatttcaaa gctgtcttag
     1081 tgcattttta gtacacaaat attttttcat ttttcttaaa ttttaataat ctaagcttaa
     1141 ataaaggctt caccgtctga aaatgcaact tttttcagat gaaatattaa aaaaaaaacc
     1201 tcttttatta attttgtgct ccaaactttc ccacggtacg caaatcatgt aatatgtact
     1261 tttaaacatt atttttccaa tcaaaaatat ccaacagctt ttccttacaa tttgaaaatt
     1321 tttattaaaa tttcaaaaaa attttttttt tttttaattt cgaatttttt taaacttgtt
     1381 gtaacttttt tccctgtcgg cgccaattaa caaaatacgt agaattttgc gcttattgtt
     1441 ttgttagtta acaaattttt gacaactttc ttatcagctg agatttaggt gtttcagttc
     1501 gcaatctgcg tctctccttg ctctcctcta ttttaaaacc cccataactt tgttcttgtg
     1561 agagttatca aaaaattgtc aactaacaaa atatagagca caaaaatatg cacattttgt
     1621 atgttgacaa ttttttgata ccttttttag ttttcgagat atcttagttt tagtttagct
     1681 gtaccttttt ctggcaaaaa acttccaaat ttcggcaatt tttcggcaaa tttcccggca
     1741 aatttcgcca agtacatcgg gctctcagac aagtggtctg tgagtggaag tcgataaaag
     1801 tagtttacgt gctctccgtc cccccgcttc ttctccgtct ctgcttctat ttctccattg
     1861 actagtccca ctacttttta cggggcggag ggctctcttg cactccagct tcttctcttt
     1921 ttgtgctctt ttttgcccgt atgtgggtgc tcatttattt ttctttggtt ttaaaataaa
     1981 ttcggtttat taggctactc gtaaaaatga agaaaaaact tcaaattcaa acgacattaa
     2041 aaaaaaattt aataaaaatt ttttgaattg ctcaaaagcc ccacctatat atatatatag
     2101 ctccgcctac ttctcgcaca gaccccgccc actttttgac tccgccccct tattggcaaa
     2161 agttcttgaa aaaaataaaa ataaaaattc aaattgaaac aatttttttt taatgaagta
     2221 gtttttttct aaattgatga aaatttctaa acagtttaca agcaccgccc actttttgac
     2281 tccgcccctt tgttggaaaa attcttgtaa aaaaaaataa aaaataaaag cttctctttc
     2341 aaacgatttt tagttggtct ttgtatgaaa agtttttttc ctaaaataga aatgtctgaa
     2401 taatttgcaa gctccgccct tttcttaccc agactccgcc cctttattga ctaatttttt
     2461 tttgtccatt tacctgaagt tcaaaaaaaa aatttacagt ttcatagagt aataattttt
     2521 caaaaatctc tttttccaat gtttaatgtg tcattataaa cccgcccagt tttaagctcc
     2581 gcccatttag gccccgccca tttatgaccc aaatattttt ttctctattc acccgtggat
     2641 ttccctccaa caagttaccc caaaaataaa aataaaaatt cctgcaggcg cccaccggga
     2701 tccggcgcgc ctctagagga tccccgggat tggccaaagg acccaaaggt atgtttcgaa
     2761 tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc gacggtacca
     2821 aaaatggtct ccaagggaga ggagcttatc aaggagaaca tgcacatgaa gctttacatg
     2881 gagggaaccg tcaacaacca ccacttcaag tgcaccaccg agggagaggg aaagccatac
     2941 gagggaaccc aaacccaacg catcaaggtc gtcgagggag gaccacttcc attcgccttc
     3001 gacatccttg ccacctgctt catgtacgga tccaagacct tcatcaacca cacccaggta
     3061 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggg aatcccagac
     3121 ttcttcaagc agtccttccc agagggattc acctgggagc gcgtcaccac ctacgaggac
     3181 ggaggagtcc ttaccgtcac ccaagacacc tcccttcaag acggatgcct tatctacaac
     3241 gtcaagcttc gtggagtcaa cttcccatcc aacggaccag tcatgcagaa gaagaccctt
     3301 ggatgggagg ccaccaccga gaccctttac ccagccgacg gaggacttga gggacgctgc
     3361 gacatggccc ttaagcttgt cggaggagga caccttcact gcaaccttaa ggtaagttta
     3421 aacagttcgg tactaactaa ccatacatat ttaaattttc agaccaccta ccgctccaag
     3481 aagccagcca agaaccttaa gatgccagga gtctacttcg tcgaccgccg ccttgagcgt
     3541 atcaaggagg ccgacaacga gacctacgtc gagcaacacg aggtcgccgt cgcccgttac
     3601 tgcgaccttc catccaagct tggacacaag cttaacggaa tggacgagct ttacaagtaa
     3661 gagctccgca tcggccgctg tcatcagatc gccatctcgc gcccgtgcct ctgacttcta
     3721 agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     3781 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     3841 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     3901 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     3961 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     4021 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt
     4081 cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat
     4141 ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt
     4201 ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca
     4261 agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg
     4321 aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa
     4381 atgacagaat acattcccaa tataccaaac ataactgttt ctactagtcg gccgtacggg
     4441 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4501 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4561 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     4621 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     4681 atcaggcggc cttaattaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4741 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4801 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4861 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4921 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4981 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5041 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5101 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5161 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5221 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5281 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5341 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5401 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5461 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5521 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5581 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5641 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5701 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5761 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5821 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6601 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6661 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6721 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6781 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6841 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6901 gaaacagct
//
