LOCUS       sequence-38814-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Watanabe N, Mitchison TJ
  TITLE     Single-molecule speckle analysis of actin filament turnover in 
            lamellipodia.
  JOURNAL   Science. 2002 Feb 8. 295(5557):1083-6.
  PUBMED    11834838
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Feb 8. 295(5557):1083-6."
COMMENT     Sequence Label: Reduced Expression GFP beta actin
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      323..332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(374..395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(635..654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
      181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      241 cttacggtaa atggcccgcc tgggaggtct atataagcag agctggttta gtgaaccgtc
      301 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      361 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      421 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      481 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      541 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      601 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      661 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      721 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactac
//
