LOCUS       sequence-388282-       15962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 genome editing plasmid in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 15962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL666
FEATURES             Location/Qualifiers
     source          1..15962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2003
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6129..6149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6162..6182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6195..6215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6226..6474
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(6736..7041)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6838..6857)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7524..7548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7578..7602
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8903..9532
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9961..11034
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11100..11294
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11530..11552)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11628..11768
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11683..11702
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11783..11800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11954..12542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12034..12053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12629..13423)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13848..13872
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13950..14124
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15214..15890)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15257..15277)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccctc
     1021 tctaccttct caagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt
     1081 tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tactagcgtt cgtacacgga
     1141 tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa
     1201 tcctgggatg gctctagccg ttccgcagac gggatcgatt tcatgatttt ttttgtttcg
     1261 ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt
     1321 cgggtcatct tttcatgctt ttttttgtct tggttgtgat gatgtggtct ggttgggcgg
     1381 tcgttcaaga tcggagtaga attaattctg tttcaaacta cctggtggat ttattaattt
     1441 tggatctgta tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa
     1501 atatcgatct aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat
     1561 gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcaa
     1621 gatcggagta gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg
     1681 tgtgtgtcat acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat
     1741 aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct
     1801 attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt
     1861 attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta
     1921 gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct
     1981 gttgtttggt gttacttctg cagcctgcag gtagatcgct cgtcgacatg acaaagctga
     2041 accagccata cggcatcggc ctcgatattg gctcaaactc tatcggcttc gcggtggtcg
     2101 acgccaattc ccacctcctg cgcctgaagg gcgagacagc catcggcgcc aggctcttcc
     2161 gcgagggaca gtcagccgcg gatcggaggg gctctaggac cacacggagg cgcctctccc
     2221 ggacaaggtg gcgcctcagc ttcctgcgcg acttcttcgc gccacatatc accaagattg
     2281 accctgattt cttcctccgc cagaagtact ccgagatcag ccctaaggac aaggatcggt
     2341 tcaagtacga gaagaggctg ttcaacgacc gcaccgatgc cgagttctac gaggattacc
     2401 catcaatgta ccacctgagg ctccatctga tgacccacac acataaggcc gacccgaggg
     2461 agattttcct cgccatccac catattctca agtctcgggg ccacttcctc acacctggag
     2521 cggccaagga tttcaatacc gacaaggtgg atctggagga catcttccca gccctcacag
     2581 aggcgtacgc ccaggtctac ccggacctgg agctgacctt cgacctcgcg aaggccgacg
     2641 atttcaaggc gaagctcctg gatgagcagg ccaccccgtc cgacacacag aaggcgctgg
     2701 tgaacctcct gctctccagc gatggcgaga aggagattgt caagaagagg aagcaggtgc
     2761 tgacagagtt cgcgaaggcc atcaccggcc tcaagacaaa gttcaacctc gccctgggca
     2821 ccgaggtgga tgaggccgat gcctcaaatt ggcagttctc tatgggccag ctcgacgata
     2881 agtggtccaa catcgagaca tccatgacag accagggcac cgagattttc gagcagattc
     2941 aggagctgta ccgggcgagg ctgctcaacg gcattgtgcc agccggcatg tccctgagcc
     3001 aggccaaggt cgccgattac ggccagcaca aggaggacct ggagctgttc aagacatacc
     3061 tcaagaagct gaatgatcat gagctggcga agaccatccg gggcctctac gacaggtaca
     3121 ttaacggcga cgatgccaag ccgttcctgc gcgaggactt cgtcaaggcg ctcaccaagg
     3181 aggtgacagc ccatccaaac gaggtgtccg agcagctgct caacaggatg ggccaggcca
     3241 atttcatgct caagcagagg accaaggcca acggcgcaat cccaattcag ctccagcagc
     3301 gcgagctgga tcagatcatt gcgaaccaga gcaagtacta cgactggctc gccgccccaa
     3361 atccagtgga ggcacacagg tggaagatgc cgtaccagct cgatgagctg ctcaatttcc
     3421 atatcccata ctacgtcggc cctctgatta caccgaagca gcaggcggag tcaggcgaga
     3481 acgtgttcgc ctggatggtc aggaaggacc cgtctggcaa catcacccca tacaatttcg
     3541 atgagaaggt ggaccgggag gcgtcagcca atacattcat ccagaggatg aagaccacag
     3601 atacctacct gattggcgag gacgtgctcc ctaagcagtc tctgctctac cagaagtacg
     3661 aggtgctcaa cgagctgaac aatgtccgca tcaacaatga gtgcctgggc acagatcaga
     3721 agcagcggct catcagggag gtgttcgagc ggcactcatc tgtgaccatt aagcaggtcg
     3781 cggataatct cgtggcacat ggcgacttcg cccggaggcc ggagatcagg ggcctggcgg
     3841 acgagaagag gttcctctcc agcctgtcca cataccacca gctcaaggag atcctgcatg
     3901 aggccattga cgatccaacc aagctgctcg atatcgagaa catcattacc tggagcacag
     3961 tgttcgagga ccacaccatc ttcgagacaa agctggccga gattgagtgg ctcgacccga
     4021 agaagatcaa cgagctgtca ggcattcgct accggggatg gggacagttc tctcgcaagc
     4081 tgctcgacgg cctcaagctg ggcaacggcc acacagtcat tcaggagctg atgctgagca
     4141 accataatct catgcagatc ctggccgatg agacactcaa ggagacaatg acagagctga
     4201 accaggacaa gctcaagaca gacgatatcg aggatgtgat taacgacgcg tacacctcac
     4261 catctaataa gaaggccctc cggcaggtcc tgagggtggt cgaggatatt aagcatgccg
     4321 cgaatggcca ggacccttca tggctcttca tcgagacagc cgacggcacc ggcacagccg
     4381 gcaagcgcac acagtctcgg cagaagcaga tccagaccgt gtacgcgaac gccgcgcagg
     4441 agctgattga ttccgccgtg aggggcgagc tggaggataa gatcgcggac aaggcctctt
     4501 tcacagaccg cctcgtgctg tacttcatgc agggcggcag ggatatctac acaggcgccc
     4561 ctctgaatat tgaccagctc tcccactacg acatcgatca tattctcccg cagtcactga
     4621 ttaaggacga ttctctggac aacagggtgc tcgtcaacgc gacaatcaat cgcgagaaga
     4681 acaatgtgtt cgcctccaca ctcttcgcgg gcaagatgaa ggcaacctgg cggaagtggc
     4741 atgaggcagg cctgatttca ggacgcaagc tccggaatct catgctgagg ccggatgaga
     4801 tcgacaagtt cgccaaggga ttcgtggcaa ggcagctggt ggagacaagg cagatcatta
     4861 agctcacaga gcagatcgct gccgcccagt accctaacac caagatcatt gccgtgaagg
     4921 cgggcctcag ccaccagctg agggaggagc tggatttccc gaagaacagg gacgtgaatc
     4981 attaccacca tgcgttcgat gccttcctcg ccgcaaggat tggcacatac ctgctcaagc
     5041 gctacccgaa gctggcgcct ttcttcacct acggcgagtt cgccaaggtg gacgtgaaga
     5101 agttcaggga gttcaacttc atcggcgcgc tcacacacgc caagaagaat atcattgcga
     5161 aggataccgg cgagattgtg tgggataagg agagggacat ccgcgagctg gacaggatct
     5221 acaatttcaa gcgcatgctc attacccatg aggtctactt cgagacagcc gatctcttca
     5281 agcagaccat ctacgccgca aaggactcca aggagagggg cggcagcaag cagctgattc
     5341 ctaagaagca gggctacccg acacaggtgt acggcggata cacccaggag tcaggcagct
     5401 acaatgcgct cgtgcgcgtg gccgaggcag ataccacagc ctaccaggtc atcaagattt
     5461 ccgcgcagaa cgcctccaag atcgcgagcg ccaatctcaa gagcagggag aagggcaagc
     5521 agctgctcaa tgagattgtg gtcaagcagc tggcgaagcg ccggaagaac tggaagcctt
     5581 cagccaattc tttcaagatc gtgattccga ggttcggcat gggcacactg ttccagaacg
     5641 ccaagtacgg cctcttcatg gtcaactcag acacctacta ccgcaattac caggagctgt
     5701 ggctgtcccg ggagaatcag aagctgctca agaagctctt cagcatcaag tacgagaaga
     5761 cacagatgaa ccacgatgcc ctccaggtgt acaaggccat cattgaccag gtcgagaagt
     5821 tcttcaagct gtacgatatc aatcagttca gggcgaagct ctccgacgcc attgagcgct
     5881 tcgagaagct gccgatcaac accgatggca ataagattgg caagaccgag acactccggc
     5941 agatcctcat tggcctccag gcgaacggca caaggtccaa cgtgaagaat ctgggcatca
     6001 agaccgacct cggcctgctc caggtcggca gcggcatcaa gctcgacaag gatacccaga
     6061 ttgtctacca gtcaccgtct ggcctgttca agaggcgcat cccactcgcc gacctgtcaa
     6121 gggctgatcc taagaagaag aggaaggttt ctagggcaga tccaaagaag aagcggaagg
     6181 tgtctagagc tgatccgaag aagaagcgca aggtgtgact cgagatatga agatgaagat
     6241 gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc
     6301 ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga tcacattata
     6361 atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc
     6421 atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga taagaggatc
     6481 cgcggatcat gaaccaacgg cctggctgta tttggtggtt gtgtagggag atggggagaa
     6541 gaaaagcccg attctcttcg ctgtgatggg ctggatgcat gcgggggagc gggaggccca
     6601 agtacgtgca cggtgagcgg cccacagggc gagtgtgagc gcgagaggcg ggaggaacag
     6661 tttagtacca cattgcccag ctaactcgaa cgcgaccaac ttataaaccc gcgcgctgtc
     6721 gcttgtgtgg agaccttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
     6781 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gaaaccggca cactggccat
     6841 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
     6901 ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg
     6961 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
     7021 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca
     7081 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
     7141 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat
     7201 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
     7261 catagcatac ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg
     7321 caaaaatcag cgcgcaaata cgcatactgt tatctggctt ggtaccggtc tcagtctcag
     7381 gtagatgtca gatcaatcag aaatgattga tctgacatct acgagttgag atcaaacaaa
     7441 gcttcagctg agtttcaatt tctgagccca tgttgggcca tacatatgcc acccgagtgc
     7501 aaatcgggtg gctttttttc tagacccagc tttcttgtac aaagttggca ttactgcgag
     7561 ctcaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     7621 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     7681 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     7741 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     7801 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     7861 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     7921 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     7981 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     8041 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     8101 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     8161 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     8221 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     8281 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     8341 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     8401 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     8461 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     8521 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     8581 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     8641 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     8701 acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     8761 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     8821 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     8881 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     8941 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     9001 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     9061 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     9121 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     9181 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     9241 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     9301 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     9361 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     9421 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     9481 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     9541 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     9601 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     9661 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     9721 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     9781 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     9841 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     9901 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     9961 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
    10021 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
    10081 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
    10141 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
    10201 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
    10261 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
    10321 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
    10381 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
    10441 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
    10501 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
    10561 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
    10621 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
    10681 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
    10741 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
    10801 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
    10861 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
    10921 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    10981 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    11041 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg cctctttcct
    11101 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    11161 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    11221 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    11281 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    11341 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    11401 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    11461 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    11521 cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga
    11581 tgccgggagc agacaagcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    11641 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
    11701 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    11761 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11821 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11881 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11941 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    12001 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    12061 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    12121 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    12181 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    12241 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    12301 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    12361 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    12421 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    12481 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    12541 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    12601 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
    12661 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    12721 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    12781 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    12841 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    12901 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    12961 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    13021 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    13081 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    13141 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    13201 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    13261 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    13321 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    13381 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    13441 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    13501 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    13561 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    13621 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    13681 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    13741 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    13801 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    13861 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    13921 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
    13981 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
    14041 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
    14101 tactcacaca ttattatgga gaaaatagag cttgtcgatc gttaattaat cacctccaag
    14161 atcccggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
    14221 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
    14281 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
    14341 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
    14401 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
    14461 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
    14521 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
    14581 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
    14641 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
    14701 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
    14761 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
    14821 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
    14881 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
    14941 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
    15001 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
    15061 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggac acgctgtcga
    15121 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatcctag
    15181 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
    15241 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
    15301 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    15361 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    15421 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    15481 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    15541 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    15601 attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg
    15661 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
    15721 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
    15781 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
    15841 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
    15901 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
    15961 tg
//
