LOCUS       sequence-388343-       11584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for use in generating DCK*-POI fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, 
            Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, 
            Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi 
            SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
  TITLE     Targeting oncoproteins with a positive selection assay for protein 
            degraders.
  JOURNAL   Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 
            10.1126/sciadv.abd6263. Print 2021 Feb.
  PUBMED    33547076
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-5"; volume: "7(6). pii: 7/6/eabd6263. doi"; pages: " 
            10.1126/sciadv.abd6263. Print 2021 Feb"
COMMENT     Sequence Label: pLX304-DCK*-GW-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      583..592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1474..1598
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1623..1628,1629..1646,1647..1653)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1707..2366
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1773..1792)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2708..3013
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2892..2911
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3054..3178)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3180..3221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(3262..3836,3837..3839,3840..3848)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3429..3446)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3656..3675
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3849..3851,3852..3854,3855..4568)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3849..3851,3852..3854,3855..4565)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3894..3915)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4155..4174)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4502..4523
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4591..5179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4644..4664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5062..5073)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5251..5484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5562..5683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5599..5618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5653..5672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5723..5858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5878..5897)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5879..5897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5907..5924)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5907..5923)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5916..5938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6065..6520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6152..6171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6362..6383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6546..6650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6651..6719,6720..7511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6869..6888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7682..8270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8171..8190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8424..8441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8558..8579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8594..8599,8600..8617,8618..8624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8632..8648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8637..8659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8656..8672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8656..8672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8691..8711
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8693..8711
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8739..8965
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8966..9146
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9193..9318
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9811..10044
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10229..10273
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10448..10948
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10867..10885
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10960..11355
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11415..11532
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aagccaccat ggttccgcgt
      601 ggctctcata tggccacccc gcccaagaga agctgcccat ctttctcagc cagctctgag
      661 gggacccgca tcaagaaaat ctccatcgaa gggaacatcg ctgcagggaa gtcaacattt
      721 gtgaatatcc ttaaacaatt gtgtgaagat tgggaagtgg ttcctgaacc tgttgccaga
      781 tggtgcaatg ttcaaagtac tcaagatgaa tttgaggaac ttacaatgga gcagaaaaat
      841 ggtgggaatg ttcttcagat gatgtatgag aaacctgaac gatggtcttt taccttccaa
      901 acctacgcct gtctcagtat gataagagct cagcttgcct ctctgaatgg caagctcaaa
      961 gatgcagaga aacctgtatt attttttgaa cgatctgtgt atagtgcgag gtatattttt
     1021 gcatctaatt tgtatgaatc tgaatgcatg aatgagacag agtggacaat ttatcaagac
     1081 tggcatgact ggatgaataa ccaatttggc caaagccttg aattggatgg aatcatttat
     1141 cttcaagcca ctccagagac atgcttacat agaatatatt tacggggaag aaatgaagag
     1201 caaggcattc ctcttgaata tttagagaag cttcattata aacatgaaag ctggctcctg
     1261 cataggacac tgaaaaccaa cttcgattat cttcaagagg tgcctatctt aacactggat
     1321 gttaatgaag actttaaaga caaatatgaa agtctggttg aaaaggtcaa agagtttttg
     1381 agtactttgg gagggggtag cggcggaggt tcaggaggcg gaagtggtgg tggctccgga
     1441 ggcggtagtg gcggaggttc actgtcggga tcaacaagtt tgtacaaaaa agctgaacga
     1501 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     1561 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     1621 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
     1681 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1741 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1801 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1861 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1921 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1981 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     2041 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     2101 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     2161 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     2221 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     2281 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     2341 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
     2401 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     2461 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     2521 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     2581 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     2641 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     2701 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     2761 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     2821 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     2881 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     2941 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     3001 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     3061 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     3121 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
     3181 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagccgct
     3241 acgtaaattc cgcccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     3301 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3361 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3421 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3481 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3541 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     3601 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     3661 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     3721 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     3781 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     3841 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3901 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3961 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4021 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4081 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4141 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4201 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4261 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     4321 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4381 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4441 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4501 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4561 acaagtaaac cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     4621 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4681 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4741 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4801 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4861 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4921 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4981 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5041 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5101 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5161 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     5221 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5281 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5461 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     5521 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     5581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5701 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5821 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     5881 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     5941 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6001 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6061 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6481 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     6541 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6601 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6661 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6721 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6781 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6841 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6901 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6961 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7021 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7081 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7141 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7201 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7261 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7321 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7381 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7441 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7501 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7561 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8341 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8401 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8461 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8521 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     8581 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     8641 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     8701 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     8761 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     8821 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     8881 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     8941 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     9001 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9061 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9121 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9181 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9241 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     9301 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     9361 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     9421 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     9481 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     9541 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     9601 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     9661 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     9721 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     9781 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     9841 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     9901 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9961 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    10021 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    10081 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    10141 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    10201 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    10261 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    10321 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    10381 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    10441 gggacccttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
    10501 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
    10561 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
    10621 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
    10681 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
    10741 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
    10801 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
    10861 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
    10921 tgaccgaatc accgacctct ctccccaggg ggtaccacca tggccaagcc tttgtctcaa
    10981 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
    11041 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
    11101 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
    11161 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
    11221 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
    11281 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
    11341 tatgtgtggg agggcctgca gctgcagtag taagaattct agatcttgag acaaatggca
    11401 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    11461 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    11521 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
    11581 gggg
//
